Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study

Matchynski, James I.; Manwar, Rayyan; Kratkiewicz, Karl; Madangopal, Rajtarun; Lennon, Veronica A; Makki, Kassem M.; Reppen, Abbey L.; Woznicki, Alexander R.; Hope, Bruce T.; Perrine, Shane A.; Conti, Alana C.; Avanaki, Kamran

doi:10.1016/j.pacs.2021.100297

Cited by 21 publications

(11 citation statements)

References 64 publications

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“…The next step is to test our spectroscopic thermoacoustic system on organic phantoms to distinguish between tissue layers based on their absorption coefficients and dielectric properties, ultimately leading to our goal of detecting tumors. It is expected that TA will widen the applications of existing similar modalities such as PA imaging [9,24,[46][47][48][49][50][51][52][53][54][55][56][57][58].…”

Section: Resultsmentioning

confidence: 99%

Development and characterization of thermoacoustic imaging system using rotational single element transducer

Islam¹,

Zafar²,

Prakash³

et al. 2023

Photons Plus Ultrasound: Imaging and Sensing 2023

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Microwave-induced thermoacoustic tomography has the advantage of a high spatial resolution and a deep imaging depth. This method has been extensively explored over the past decade to find an alternative of existing imaging techniques. In this study, we have developed a compact microwave-induced thermoacoustic tomography (MI-TAT) with a waveguide antenna and a rotating ultrasound transducer unit. We performed a characterization study of the system in terms of pulse width, selection of microwave frequency and resolution. Then the optimized parameters were used to image in-vitro complex structure phantoms. Later, we expanded our system capability for spectroscopic study by imaging different concentrations of methanol and water to mimic the tissue properties and analyze them based on the absorption characteristics of these materials. We hope, this spectroscopic capability broadens the capability of thermoacoustic system to separate the diseased tissue from the healthy one (e.g., malignant from benign) with a high sensitivity and specificity.

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Section: Resultsmentioning

confidence: 99%

Development and characterization of thermoacoustic imaging system using rotational single element transducer

Islam¹,

Zafar²,

Prakash³

et al. 2023

Photons Plus Ultrasound: Imaging and Sensing 2023

Self Cite

View full text Add to dashboard Cite

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“…In recent decades, numerous imaging techniques have been developed to provide fast and accurate diagnosis of brain disorders [1][2][3][4][5][6][7][8][9][10][11][12]. One such technique is ultrasound imaging, which has gained the attention of the medical community for its ability to detect brain injuries and anomalies [13,14] using low-cost, user-friendly setups [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…This reduces our capability to correct aberrations and efficiently use non-invasive imaging method like TCUS. Some other imaging modalities such photoacoustic imaging (both in tomography and microscopy) [12,[42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57] also suffers from such limitations.…”

Section: Introductionmentioning

confidence: 99%

Relationship between skull phantoms physical characteristics and acoustic wave signal features

Saint-Martin¹,

Aggrawal²,

Manwar³

et al. 2023

Photons Plus Ultrasound: Imaging and Sensing 2023

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During medical investigations of the head, ultrasound measurements can offer information with simple, non-invasive, and real-time procedure. However, for human adult applications, the clinical treatment of transcranial acoustic imaging remains a challenge by the presence of the skull, results in acoustic aberrations caused by two main phenomena, i.e., attenuation and distortion. These aberrations may affect the signal understanding because of the induced artifacts and the inaccuracy of the imaging target structural information. Variations of the physical properties of the skull, its thickness and porosity, will strongly affect the mechanical properties of the medium and thus the acoustic response. We propose a method to understand the influence of these characteristics on the signal degradation. In order to mimic the human adult skull, a large quantity of epoxy resin-based phantoms is created to explore all the possible physical characteristic variation in the bone. Additional components, titanium dioxide and seeds, will be added to the samples to recreate the acoustic scattering effects of a skull bone. Signal features from pulse-echo mode ultrasound, such as signal attenuation or broadening, will be extracted and studied in the time and frequency domain. In this paper, we are looking for relationship between these physical parameters and the signal features, with the objective to determine bone characteristics without any direct access in later experiments; and going a step further into aberration correction during transcranial imaging procedure.

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“…The absorption distribution across the tissue is then used to reconstruct either a two-dimensional (2D) or three-dimensional (3D) imaging, depending on configuration of PAI system [2][3][4][5][6]. Many configurations of PAI have been implemented for fine or coarse resolution imaging [3,5,[7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Photoacoustic computed tomography (PACT), one of several PAI frameworks, provides features that aid in clinical applicability.…”

Section: Introductionmentioning

confidence: 99%

Transducer misplacement compensation for in-lab-made 3D photoacoustic tomography systems using nature-inspired algorithms

Rahaman¹,

Prakash

Ranjbaran

et al. 2023

Photons Plus Ultrasound: Imaging and Sensing 2023

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Three-dimensional (3D) photoacoustic tomography is a method of choice for imaging round organs such as brain and breast. Many research groups have used a fully populated hemispherical transducer array with 256, 512, 1028, or 2048 elements and used that for 3D imaging. These transducer arrays are expensive and require a sophisticated data acquisition unit. Other groups have used much smaller number of transducers with a rotating mechanism which eventually filled out the entire hemisphere. We have built a 3D hemispherical array with 28 transducers which are placed on a 3D printed dome-like unit. The location of transducers however may be off-placed by a few millimeters (due to human error and errors in 3D printing). This may be to defocus the reconstructed image if the acceptable positions of transducers are not selected. In this work, we developed a compensation algorithm for misplacement of these transducers using Cuckoo search (CS) algorithm. The CS algorithm finds the optimum location for the transducers using levy flight which relies on levy distribution. The optimum location of each of these transducers is found within -4 mm to 4 mm of their locations. Universal back projection algorithm was used for image reconstruction and the sharpness of 3D image was used as the cost function; additionally, two more objective functions, the Brenner gradient, and the Tenenbaum gradient was investigated.

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Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study

Cited by 21 publications

References 64 publications

Development and characterization of thermoacoustic imaging system using rotational single element transducer

Development and characterization of thermoacoustic imaging system using rotational single element transducer

Relationship between skull phantoms physical characteristics and acoustic wave signal features

Transducer misplacement compensation for in-lab-made 3D photoacoustic tomography systems using nature-inspired algorithms

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