Direct measurement of multi-elements in high matrix samples with a flow injection ICP-MS: application to the extended<i>Emiliania huxleyi</i>Redfield ratio

Zhang, Qiong; Snow, Joseph T.; Holdship, Phil; Price, David; Watson, Paul; Rickaby, Rosalind E. M.

doi:10.1039/c8ja00031j

Cited by 20 publications

(30 citation statements)

References 53 publications

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“…We did not observe difference in growth rate in cultures with equivalent [Fe′] (7500 pmol L −1 ) but with varying [EDTA]; cultures with 12 µmol L −1 EDTA, relative to regular SCM media with 7.5 µmol L −1 [EDTA], did not produce an inhibitory effect (data not shown). [Fe′] in cultures was calculated using Visual Minteq software [33], including background Fe contamination of 50 nmol L −1 from basal SCM medium with included macronutrients and vitamins but without added trace metals, determined using inductively coupled plasma mass spectrometry [34]. Cultures were acclimated to metal concentrations stated by transferring cultures consecutively into new media until growth rates did not vary with statistical significance (ANOVA, p < 0.05).…”

Section: Methodsmentioning

confidence: 99%

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Iron requirements and uptake strategies of the globally abundant marine ammonia-oxidising archaeon, Nitrosopumilus maritimus SCM1

et al. 2019

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Ammonia-oxidising archaea (AOA) mediate the rate-limiting step of nitrification, the central component of the marine nitrogen cycle that converts ammonia to nitrite then nitrate. Competition with phytoplankton for ammonium and light inhibition are considered to restrict AOA activity to below the photic zone, but observations of surface nitrification now demand a further understanding of the factors driving AOA distribution and activity. Pico- to nanomolar concentrations of iron (Fe) limit the growth of microorganisms in a significant portion of the world’s surface oceans, yet there is no examination of the role of Fe in AOA growth despite the process of ammonia oxidation being considered to rely on the micronutrient. Here we investigate the Fe requirements and Fe uptake strategies of the Nitrosopumilus maritimus strain SCM1, a strain representative of globally abundant marine AOA. Using trace metal clean culturing techniques, we found that N. maritimus growth is determined by Fe availability, displaying a free inorganic Fe (Fe′) half saturation constant 1–2 orders of magnitude greater for cell growth than numerous marine phytoplankton and heterotrophic bacterial species driven by a reduced affinity for Fe′. In addition, we discovered that whilst unable to produce siderophores to enhance access to Fe, N. maritimus is able to use the exogenous siderophore desferrioxamine B (DFB), likely through a reductive uptake pathway analogous to that demonstrated in phytoplankton. Our work suggests AOA growth in surface waters may be Fe limited and advances our understanding of AOA physiology on the cellular and mechanistic levels with implications for ecosystem dynamics and the biogeochemical N-cycle.

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Section: Methodsmentioning

confidence: 99%

“…HNO 3 was added to reflux for an hour to resuspend the dried samples. 56 Fe and 31 P concentrations were determined using a Quadrupole ICP-MS Perkin Elmer NexION 350 with Elemental Scientific Flow Injection Auto-sampler (FIAS) in a method optimised for high salt matrixes [34].…”

Section: Methodsmentioning

confidence: 99%

Iron requirements and uptake strategies of the globally abundant marine ammonia-oxidising archaeon, Nitrosopumilus maritimus SCM1

et al. 2019

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“…The cells were then washed two times with chelexed synthetic ocean water (made according to the methods of Morel et al ) and one time with Tris buffer (pH = 8.2) in order to remove the loosely bound metals on cell surfaces. The pellets were then digested in HNO 3 and H 2 O 2 and metal contents were measured using the ICP‐MS, according to the methods of Zhang et al ().…”

Section: Methodsmentioning

confidence: 99%

“…Last, the pellet containing the MRG was digested with in‐house distilled HNO 3 and ultrapure H 2 O 2 . The respective solutions containing the protein, CD, and MRG fractions were then measured on the ICP‐MS, again, according to the methods of Zhang et al ().…”

Section: Methodsmentioning

confidence: 99%

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Susceptibility of algae to Cr toxicity reveals contrasting metal management strategies

Wilson

Zhang

Rickaby

2019

Limnology & Oceanography

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At the Paleozoic–Mesozoic boundary, the dominance of marine eukaryotic algae shifted from the green (chlorophyll b) to the red (chlorophyll c) superfamily. Selection pressures caused by the bioavailability of trace metals associated with increasing oxygenation of the ocean may have played a key role in this algal revolution. From a scan of elemental compositions, a significant difference in the cellular Cr/P quota was found between the two superfamilies. Here, the different responses to high levels of Cr exposure reveal contrasting strategies for metal uptake and homeostasis in these algal lineages. At high Cr(VI) concentrations, red lineages experience growth inhibition through reduced photosynthetic capability, while green lineages are completely unaffected. Moreover, Cr(VI) has a more significant impact on the metallomes of red lineage algae, in which metal/P ratios increased with increasing Cr(VI) concentration for many trace elements. Green algae have higher specificity transporters to prevent Cr(VI) from entering the cell, and more specific intracellular stores of Cr within the membrane fraction than the red algae, which accumulate more Cr mistakenly in the cytosol fraction via lower affinity transport mechanisms. Green algal approaches require greater nutrient investments in the more numerous transport proteins required and management of specific metals, a strategy better adapted to the resource‐rich coastal waters. By contrast, the red algae are nutrient‐efficient with fewer and less discriminate metal transporters, which can be fast and better adapted in the oligotrophic, oxygenated open ocean, which has prevailed since the deepening of the oxygen minimum zones at the start of the Mesozoic era.

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Strategies to overcome interferences in elemental and isotopic geochemical analysis by quadrupole inductively coupled plasma mass spectrometry: A critical evaluation of the recent developments

Balaram

2021

Rapid Comm Mass Spectrometry

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Quadrupole Inductively Coupled Plasma Mass Spectrometry (ICP‐MS) instruments were introduced into geochemical and mineral exploration laboratories nearly four decades ago, providing a technique that could meet their longstanding requirement for the precise and accurate determination of several groups of trace elements and isotopes in geological materials such as rocks, minerals, ores, soils, sediments, and natural water samples. Despite its popularity among geochemists, the technique suffered from spectral and non‐spectral interferences some of which seriously affected the quality of the data generated. These interferences have also had a significant impact on the ability of ICP‐MS systems to achieve low detection limits. Over the last three decades, technical advances such as the development of high‐resolution (HR)‐ICP‐MS, cool plasma, collision/reaction cell technology (CCT), dynamic reaction cell (DRC) technology, collision reaction interface (CRI), kinetic energy discrimination (KED), tandem mass spectrometry (ICP‐MS/MS)/triple quadrupole ICP‐MS, and multi‐quadrupole ICP‐MS have been introduced to eliminate/minimize many of these interferences, with each technique having its strengths and limitations. These technologies have extended the range of elements that can be measured accurately not only in geological materials, but also in several other matrices, with lower detection limits than before. In addition, other methods such as internal standardization, isotope‐dilution, standard addition and matrix‐matching calibrations have contributed to improving the quality of the data. This paper provides a review of these new developments from the geochemical analysis point of view.

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Direct measurement of multi-elements in high matrix samples with a flow injection ICP-MS: application to the extendedEmiliania huxleyiRedfield ratio

Abstract: Based on our novel ICP-MS method, we find that the metalloproteins are a better indicator of metal requirements of phytoplankton compared with whole cell metal quotas.

Cited by 20 publications

References 53 publications

Iron requirements and uptake strategies of the globally abundant marine ammonia-oxidising archaeon, Nitrosopumilus maritimus SCM1

Iron requirements and uptake strategies of the globally abundant marine ammonia-oxidising archaeon, Nitrosopumilus maritimus SCM1

Susceptibility of algae to Cr toxicity reveals contrasting metal management strategies

Strategies to overcome interferences in elemental and isotopic geochemical analysis by quadrupole inductively coupled plasma mass spectrometry: A critical evaluation of the recent developments

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