Direct measurement of interstitial pulmonary pressure in in situ lung with intact pleural space

Miserocchi, G.; Negrini, Daniela; Gonano, C.

doi:10.1152/jappl.1990.69.6.2168

Cited by 70 publications

(59 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The method has been evaluated by Wiig et al (1981). The system used here was as described by Miserocchi et al (1990). Briefly, glass micropipettes of outer diameter 1 mm with ground, beveled tips of diameter of 2-5 m were filled with 1 M NaCl, which was colored with lissamine green for tip visualization.…”

Section: Interstitial Pressure Measurements By the Servonull Micropipmentioning

confidence: 99%

Influence of Actin Cytoskeleton on Intra-articular and Interstitial Fluid Pressures in Synovial Joints

Poli

Scott

Bertin

et al. 2001

Microvascular Research

Self Cite

View full text Add to dashboard Cite

Fibroblast microfilamentous actin (F-actin) influences interstitial fluid pressure via linkages to collagen in rat skin (Berg et al., 2001). The present aims were to determine whether the actin cytoskeleton of synovial endothelium, fibroblasts, and synoviocytes influences in vivo (i) fluid exchange between a joint cavity and synovial microcirculation and (ii) extracellular fluid pressures in joints. Rabbit knee joints were treated intra-articularly with the Factin disrupting drugs cytochalasin D and latrunculin B while joint fluid pressure P j was recorded. In joints injected with small volumes of control solution, P j fell with time (؊0.05 ؎ 0.01 cm H 2 O min ؊1 , mean ؎ SEM, n ‫؍‬ 9, equivalent drainage rate 3.9 l min ؊1 ). Cytochalasin or latrunculin reversed this in ϳ4 min in vivo; P j increased with time, e.g., ؉0.12 ؎ 0.04 cm H 2 O min ؊1 at 200 M cytochalasin (equivalent filtration rate into joint 6.6 -12.5 l min ؊1 , n ‫؍‬ 4), with a cytochalasin EC 50 of 45 M. Plasma ␥-globulin clearance into the joint cavity was also increased. Post mortem, cytochalasin did not reverse dP j /dt and had no more effect on P j than did control solution. Also, when synovial interstitial fluid pressures were measured by servonull micropipette post mortem (control ؊0.95 ؎ 0.37 cmH 2 O, n ‫؍‬ 18) cytochalasin had no significant effect on interstitial pressure over 60 min, even at 1 mM. It was concluded that synovial endothelial F-actin has an important role in the normal synovial microvascular resistance to fluid filtration and plasma ␥-globulin permeation and is thus a potential link between pro-in-

show abstract

Section: Interstitial Pressure Measurements By the Servonull Micropipmentioning

confidence: 99%

Influence of Actin Cytoskeleton on Intra-articular and Interstitial Fluid Pressures in Synovial Joints

Poli

Scott

Bertin

et al. 2001

Microvascular Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, in humans, there is the suggestion that some filtration may occur from the visceral pleura, as its blood supply stems from the systemic circulation where functional hydrostatic pressure is higher compared to pulmonary circulation; this fact however, may be of no relevance as the key variable in this respect is pulmonary interstitial pressure. The latter was directly measured in rabbits, by using the micropuncture technique through the pleural window approach, and was found to be rather subatmospheric (≈-10 cmH 2 O [26]). The same technique made it possible to describe the pulmonary microvascular pressure profile [27].…”

Section: Pleural Fluid Filtrationmentioning

confidence: 99%

“…The figure also shows that liquid filters from the pulmonary capillaries into the lung interstitium, from where it is removed by the lymphatics. The latter point also represents a new piece of knowledge [26], based strongly on interstitial pressure measurements performed with lungs physiologically expanded in the pleural space. The important concept to grasp here is that the lymphatics represent a drainage mechanism, able to generate a subatmospheric pressure (like a vacuum cleaner).…”

Section: Pleural Fluid Drainagementioning

confidence: 99%

“…In the lung parenchyma, for example, a very tight control exists to guarantee a minimum amount of interstitial fluid [26,29] which is crucial to assure gas diffusion. The comparison between pleural space and lung interstitium offers, in fact, an important clue to the understanding of how a condition of minimum interstitial volume is achieved.…”

Section: Control and Pathophysiologymentioning

confidence: 99%

See 1 more Smart Citation

Physiology and pathophysiology of pleural fluid turnover

Miserocchi¹

1997

Eur Respir J

Self Cite

241

128

View full text Add to dashboard Cite

Number 1 in this SeriesA review on pleural space is certainly most welcome, for the simple reason that on opening a textbook of physiology the concepts concerning pleural fluid turnover date back to 1927 [1]. The same comment, in fact, applies in general to microvascular water and solute exchange. This appears particularly misleading, considering the major advances achieved in the field over the last 70 yrs. Around the turn of the 19th century, STARLING and TUBBY [2] interpreted microvascular fluid and solute exchange as resulting from the balance between hydraulic and colloidosmotic pressures. This concept is still valid today, but the complete formulation of transmicrovascular exchange has become much more complex because water crosses biological membranes more easily than large solutes (namely, plasma proteins) do [3]. In fact, different equations describe water and solute fluxes [4].As a result, the general model of transcapillary fluid exchange still taught to students, based on fluid filtration at the arteriolar end of the capillary bed and reabsorption at the venular end, appears rather simplistic. Due to the different permeability to water and solutes, one could predict on a mathematical basis that such a model would lead to a progressive increase in interstitial protein concentration over time [3,4], a condition that cannot be confirmed experimentally.Similarly, the old hypothesis claiming that pleural fluid filters at parietal level and is reabsorbed through the visceral pleura [1] would imply that pleural liquid protein concentration would keep increasing with age, but again there are no indications that this occurs. The existence of partial restriction to the movement of large solutes, compared to that of water molecules, posed scientists the major problem of explaining how interstitial volume and protein concentration are kept fairly steady. Ideologically, this led to re-evaluation of lymphatics as the major route for interstitial fluid drainage. In fact, since lymphatics do not sieve proteins, they leave the interstitial protein concentration unaltered the latter depending only upon the sieving properties of the filtering membrane. Accordingly, the description of interstitial fluid homeostasis under steady state conditions is now being explained as a balance between capillary filtration and lymphatic absorption. Major validation of this model has come from the accumulating experimental data over the past 30 yrs concerning interstitial tissues [4] and serous spaces [5]. Interestingly, since the pioneering, work of STARLING and TUBBY [2] the pleural space has been used as a useful experimental model to study the interaction between microvascular filtration and lymphatic drainage.This article presents an integrated view of pleural fluid turnover, based on data gathered from experimental studies on animals over the last 15 yrs. The situation in man is, unfortunately, still poorly defined; yet, where possible, Under physiological conditions, the lung interstitium and the pleural space behave as function...

show abstract

“…The method used here was described fully by Miserocchi et al (1990). Briefly, the micropipettes were filled with 1.0 M NaCl solution coloured with 0.2 mg ml-' lissamine green (Sigma) for visualisation during insertion.…”

Section: Measurement Of Interstitial Fluid Pressurementioning

confidence: 99%

Interstitial pressure gradients around joints; location of chief resistance to fluid drainage from the rabbit knee

Scott

Bertin

Poli

et al. 2001

Experimental Physiology

View full text Add to dashboard Cite

The hypothesis has been advanced that synovium offers the main resistance to fluid escape from joints, even though it is under 20 pm thick. To test this, fluid was infused into the knee joint cavity of anaesthetised rabbits to set up a pressure gradient, then the profde of periarticular interstitial fluid pressure (Pif) was measured by advancing a micropipette, connected to a servo-null pressure recorder, in steps through a periarticular tissue 'window' until the joint cavity was entered. With intra-articular pressure (Pi) raised to 15 cmHzO (the pressure of an acute joint effusion) the pressure gradient d P & x (where x is distance) across the synovial lining was 0.47 f 0.04 cmH20 pm-' (n = 10 joints). This was 23.5-fold greater than the gradient in the subsynovinm (0.02 & 0.01 cmHzO ,urn-'; P < 0.0001, Student's t test), indicating that the hydraulic resistivity of the subsynovium is 14% of that of the synovium. The pressure profde was not altered by circulatory arrest. To test the hypothesis further, the effect of a stab perforation of the synovial lining on fluid drainage rate a) was studied. Perforation raised both & and the conductance term d&dPj more than 10-fold (n = 6 joints; P < O.OOO1, ANOVA). The results thus support the view that, despite its thinness, the synovial lining offers the main hydraulic resistance to fluid drainage from a synovial joint. Experimental Physiology (2001) 86.6,739-747.

show abstract

Direct measurement of interstitial pulmonary pressure in in situ lung with intact pleural space

Cited by 70 publications

References 0 publications

Influence of Actin Cytoskeleton on Intra-articular and Interstitial Fluid Pressures in Synovial Joints

Influence of Actin Cytoskeleton on Intra-articular and Interstitial Fluid Pressures in Synovial Joints

Physiology and pathophysiology of pleural fluid turnover

Interstitial pressure gradients around joints; location of chief resistance to fluid drainage from the rabbit knee

Contact Info

Product

Resources

About