Direct measurement of intercellular CO2 concentration in a gas-exchange system resolves overestimation using the standard method

Tominaga, Jun; Shimada, Hiroshi; Kawamitsu, Yoshinobu

doi:10.1093/jxb/ery044

Cited by 46 publications

(24 citation statements)

References 39 publications

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“…The controlled closure or opening of the stomata is a strategy of plants to regulate the flow of water and O 2 /CO 2 into and out of the leaf [22,23,36]; so that if the stomata remain closed, the leaf will lose less water, but will also absorb less CO 2 , which will reduce the rate of photosynthesis [8,18,37]. The opening of stomata during the appropriate periods of vapor pressure is a mechanism that genotypes use to avoid water loss due to a gradient between air in the stomata versus atmosphere thus regulating photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Purple Passion Fruit, Passiflora edulis Sims f. edulis, Variability for Photosynthetic and Physiological Adaptation in Contrasting Environments

2019

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Purple passion fruit (Passiflora edulis Sims f. edulis) is a tropical juice source. The goal of this project was to evaluate photosynthetic and physiological variability for the crop with the hypotheses that landraces contain the diversity to adapt to higher elevation nontraditional growing environments and this is dependent on specific parameters of ecological adaptation. A total of 50 genotypes of this crop were chosen from divergent sources for evaluations of their eco-physiological responses in two equatorial locations at different altitudes in the Andes Mountains, a center of diversity for the species. The germplasm included 34 landraces, 8 commercial cultivars, and 8 genebank accessions. The two locations were contrasting in climates, representing mid and high elevations in Colombia. Mid-elevation valleys are typical regions of production for passion fruit while high elevation sites are not traditional. The location effects and variables that differentiated genotypes were determined. Results showed statistically significant differences between locations and importance of physiological parameters related to photosynthesis and water use efficiency. Some landraces exhibited better water status and gas exchange than commercial types. Parameters like maximum photosynthesis, points of light saturation and compensation, darkness respiratory rate, and apparent quantum yield varied between genotype groups. The landraces, commercial types, and genebank entries also differed in content of carotenoids and chlorophylls a and b. Meanwhile, photosynthesis measurements showed that altitudinal difference had an effect on genotype-specific plant growth and adaptation. An important conclusion was that landraces contained the diversity to adapt to the new growing environment at higher altitudes.

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Section: Discussionmentioning

confidence: 99%

Purple Passion Fruit, Passiflora edulis Sims f. edulis, Variability for Photosynthetic and Physiological Adaptation in Contrasting Environments

2019

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“…It is important to consider the implications of g s for RACiR and A/C i measurements. Several studies (Boyer, 2015a(Boyer, , 2015bTominaga & Kawamitsu, 2015;Tominaga, Shimada, & Kawamitsu, 2018) show that C i is overestimated due to cuticular conductance to water. Due to the rate of RACiR, the bias in C i due to cuticle conductance should be less variable relative to standard A/C i measurements, because g s is less variable.…”

Section: Caveats For Racir In the Li-6400mentioning

confidence: 99%

Using the rapid A‐C_i response (RACiR) in the Li‐Cor 6400 to measure developmental gradients of photosynthetic capacity in poplar

Lawrence

Stinziano

Hanson

2018

Plant Cell & Environment

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The rapid A‐Ci response (RACiR) technique alleviates limitations of measuring photosynthetic capacity by reducing the time needed to determine the maximum carboxylation rate (Vcmax) and electron transport rate (Jmax) in leaves. Photosynthetic capacity and its relationships with leaf development are important for understanding ecological and agricultural productivity; however, our current understanding is incomplete. Here, we show that RACiR can be used in previous generation gas exchange systems (i.e., the LI‐6400) and apply this method to rapidly investigate developmental gradients of photosynthetic capacity in poplar. We compared RACiR‐determined Vcmax and Jmax as well as respiration and stomatal conductance (gs) across four stages of leaf expansion in Populus deltoides and the poplar hybrid 717‐1B4 (Populus tremula × Populus alba). These physiological data were paired with leaf traits including nitrogen concentration, chlorophyll concentrations, and specific leaf area. Several traits displayed developmental trends that differed between the poplar species, demonstrating the utility of RACiR approaches to rapidly generate accurate measures of photosynthetic capacity. By using both new and old machines, we have shown how more investigators will be able to incorporate measurements of important photosynthetic traits in future studies and further our understanding of relationships between development and leaf‐level physiology.

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“…significantly disturbed when gs was suppressed at pF 3.8 or more (Figures 3(d, h and 5j)). The fluctuation in C i was probably an error problem with the calculation formula (Tominaga & Kawamitsu, 2015a, 2015bTominaga, Shimada & Kawamitsu, 2018).…”

Section: Discussionmentioning

confidence: 99%

Responses of leaf gas exchange rate to acute soil drying in Jatropha curcas L

Nakabaru

Hoang

Watanabe

et al. 2020

Plant Production Science

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Development of a water-efficient irrigation system is essential in order to cultivate Jatropha curcas, a bioenergy crop, in semiarid regions. In the present study, to determine the useful parameter for the efficient irrigation system, jatropha plants were grown in pots and root boxes under greenhouse conditions in Okinawa, Japan. At 6 to 9 months after transplanting, we measured the gas exchange rate during ongoing soil water deficits and attempted to identify the threshold of soil matric potential (pF) with changes of both intrinsic water use efficiency (A/gs) as a stomatal-based index and carboxylation efficiency (A/C i) as an index of non-stomatal limitation. When irrigation was stopped, A and gs began to decline, with each parameter reaching zero at pF 4.0. However, gs was more sensitive to soil water deficits than A. After re-watering, stomatal openings and carbon uptake recovered to the value observed before water stress treatment. At pF 3.5 and higher values (representing more severe water stress), A/gs intended to increase and A/C i sharply decreased. These results suggested that approximately pF 3.5 in the soil was the threshold of non-stomatal limitations to photosynthetic rate and should be useful to the re-watering point for the water-efficient irrigation system in jatropha.

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Direct measurement of intercellular CO2 concentration in a gas-exchange system resolves overestimation using the standard method

Abstract: Obtaining leaf intercellular CO2 concentration by direct measurement, which is affected by neither cuticle properties nor stomatal behavior, is better than by calculation, which largely neglects cuticular gas transport when stomatal gas transport is restricted

Cited by 46 publications

References 39 publications

Purple Passion Fruit, Passiflora edulis Sims f. edulis, Variability for Photosynthetic and Physiological Adaptation in Contrasting Environments

Purple Passion Fruit, Passiflora edulis Sims f. edulis, Variability for Photosynthetic and Physiological Adaptation in Contrasting Environments

Using the rapid A‐C_i response (RACiR) in the Li‐Cor 6400 to measure developmental gradients of photosynthetic capacity in poplar

Responses of leaf gas exchange rate to acute soil drying in Jatropha curcas L

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Direct measurement of intercellular CO2 concentration in a gas-exchange system resolves overestimation using the standard method

Abstract: Obtaining leaf intercellular CO2 concentration by direct measurement, which is affected by neither cuticle properties nor stomatal behavior, is better than by calculation, which largely neglects cuticular gas transport when stomatal gas transport is restricted

Cited by 46 publications

References 39 publications

Purple Passion Fruit, Passiflora edulis Sims f. edulis, Variability for Photosynthetic and Physiological Adaptation in Contrasting Environments

Purple Passion Fruit, Passiflora edulis Sims f. edulis, Variability for Photosynthetic and Physiological Adaptation in Contrasting Environments

Using the rapid A‐Ci response (RACiR) in the Li‐Cor 6400 to measure developmental gradients of photosynthetic capacity in poplar

Responses of leaf gas exchange rate to acute soil drying in Jatropha curcas L

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Using the rapid A‐C_i response (RACiR) in the Li‐Cor 6400 to measure developmental gradients of photosynthetic capacity in poplar