Direct measurement of cell detachment force on single cells using a new electromechanical method

Francis, Gregory; Fisher, Len; Gamble, R. A.; Gingell, D.

doi:10.1242/jcs.87.4.519

Cited by 51 publications

(7 citation statements)

References 10 publications

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“…The results were found to be consistent with an electrostatic repulsion between the interfaces. Similar techniques were used earlier to explore the interactions between model deformable biological surfaces. − A novel technique based on alignment of paramagnetic particles in a magnetic field has also been developed to directly measure the forces between emulsion droplets. Repulsive electrostatic forces in solutions containing charged surfactants were again observed …”

Section: Introductionmentioning

confidence: 99%

Surface Forces and Deformation at the Oil−Water Interface Probed Using AFM Force Measurement

et al. 1999

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The immobilization of droplets of a hydrocarbon liquid (n-decane) on a flat solid surface has allowed the forces of interaction between a silica colloidal particle and the hydrocarbon−water interface to be measured using the atomic force microscope. Results are presented which demonstrate that nonpolar surfaces prepared in this way acquire a significantly negative diffuse layer potential in electrolyte solutions, as indicated by force distance relationships which obey DLVO theory at large separations. At smaller separations, deviations from DLVO theory are observed, followed by an attractive force which causes the instantaneous engulfment of the silica particle by the droplet. The presence of trace quantities of the anionic surfactant sodium dodecyl sulfate (SDS) radically alters the forces between the two surfaces, creating a significant barrier to engulfment, and an apparent “softening” of the fluid interface.

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Section: Introductionmentioning

confidence: 99%

Surface Forces and Deformation at the Oil−Water Interface Probed Using AFM Force Measurement

et al. 1999

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“…The force exerted on the oil drop and the radius and thickness of the thin emulsion film formed at “contact” can be determined directly. The instrument, which contains a number of features in common with an apparatus developed to examine the adhesion forces between biological cells and solid surfaces, enables the variation of disjoining pressure with film thickness to be measured for emulsion films of radius of only a few micrometers, close in size to films formed in actual emulsion systems. The novel design of the instrument allows independent control of the movement of the oil drop relative to the oil−water interface and thus may be used to investigate dynamic effects associated with drop movement.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Force−Distance Relationship for a Small Liquid Drop Approaching a Liquid−Liquid Interface

et al. 1996

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We describe a novel apparatus for the investigation of the forces between two liquid surfaces. In the configuration described here, an oil drop is formed at the tip of a thin flexible micropipet. The force exerted on the oil drop as it is pressed up to an oil-water interface is determined from the deflection of the pipet shaft. The disjoining pressure in the thin, oil-water-oil emulsion film formed by contact of the drop with the oil-water interface is determined by the hydrostatic pressure applied to the oil contained in the micropipet. The radius and the thickness of the film is derived from the optical interference pattern observed using a microscope. A simple theory is presented for the variation in force and film radius as the drop is moved up into the interface. Experimental results are given for dodecane-water-dodecane emulsion films stabilized by the anionic surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT). Data for the force and film radius as a function of the pipet position relative to the oil-water monolayer show good agreement with theoretical predictions. The variation of disjoining pressure with film thickness for the emulsion films is in accord with electrostatic theory.

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“…The coated microsphere can be replaced with another coated microbead or a cell depending on the objective of the experiment and gaseous material delivery (Lewis et al, 1999) and protein printing (Taha et al, 2003). Francis et al (1987) measured single cell adhesion by applying suction with a glass micropipette and tracking the movement with an interference reflection microscope (which is similar to an AFM). Other detection schemes (non-AFM based) with glass micropipettes have been used for cell adhesion measurements (Bowers et al, 1989;Chesla et al, 1998;Moussy, Neumann, & Zingg, 1990;Palmer et al, 2008).…”

Section: Fluidic Microcantilever-the Key Component Of Fluidfmmentioning

confidence: 99%

Extending applications of AFM to fluidic AFM in single living cell studies

Qiu

Chien

Maroulis

et al. 2022

Journal Cellular Physiology

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In this article, a review of a series of applications of atomic force microscopy (AFM) and fluidic Atomic Force Microscopy (fluidic AFM, hereafter fluidFM) in single-cell studies is presented. AFM applications involving single-cell and extracellular vesicle (EV) studies, colloidal force spectroscopy, and single-cell adhesion measurements are discussed. FluidFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. In this review, capacities of fluidFM are discussed to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces of endothelial or epithelial cells in a confluent cell monolayer in an appropriate physiological environment, and(3) the ease of measurement of vertical binding forces of intercellular adhesion between heterogeneous cells. Furthermore, key applications of fluidFM are reviewed regarding to EV absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

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Direct measurement of cell detachment force on single cells using a new electromechanical method

Cited by 51 publications

References 10 publications

Surface Forces and Deformation at the Oil−Water Interface Probed Using AFM Force Measurement

Surface Forces and Deformation at the Oil−Water Interface Probed Using AFM Force Measurement

Investigation of the Force−Distance Relationship for a Small Liquid Drop Approaching a Liquid−Liquid Interface

Extending applications of AFM to fluidic AFM in single living cell studies

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