Direct Mass Spectrometry of Polymers

Montaudo, Giorgio

doi:10.1002/pi.4980180406

Cited by 65 publications

(48 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…M atrix-assisted laser desorption/ionization (MALDI) mass spectrometry [1][2][3][4] has become an important technique to characterize the chemical structure of many different analytes, including industrial polymer materials [5][6][7][8][9][10]. MALDI can generate important data on the telomer repeat units, end groups, and average molecular weights of these materials.…”

mentioning

confidence: 99%

Extending the solvent-free MALDI sample preparation method

Hanton

Parees

2005

J. Am. Soc. Mass Spectrom.

112

View full text Add to dashboard Cite

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize many different materials, including synthetic polymers. MALDI mass spectral data can be used to determine the polymer average molecular weights, repeat units, and end groups. One of the key issues in traditional MALDI sample preparation is making good solutions of the analyte and the matrix. Solvent-free sample preparation methods have been developed to address these issues. Previous results of solvent-free or dry prepared samples show some advantages over traditional wet sample preparation methods. Although the results of the published solvent-free sample preparation methods produced excellent mass spectra, we found the method to be very time-consuming, with significant tool cleaning, which presents a significant possibility of cross contamination. To address these issues, we developed an extension of the solvent-free method that replaces the mortar and pestle grinding with ball milling the sample in a glass vial with two small steel balls. This new method generates mass spectra with equal quality of the previous methods, but has significant advantages in productivity, eliminates cross contamination, and is applicable to liquid and soft or waxy analytes. (J Am Soc Mass Spectrom 2005, 16, 90 -93)

show abstract

mentioning

confidence: 99%

Extending the solvent-free MALDI sample preparation method

Hanton

Parees

2005

J. Am. Soc. Mass Spectrom.

112

View full text Add to dashboard Cite

show abstract

“…In these experiments we show the CID fragmentation mass spectra on some ethoxylated surfactants, and demonstrate the existence of analyte matrix clusters. [5][6][7]. MALDI can generate important data on the telomer repeat units, end groups, and average molecular weights of these materials.…”

mentioning

confidence: 99%

“…M atrix-assisted laser desorption/ionization (MALDI) mass spectrometry [1][2][3][4] has become an important technique to characterize the chemical structure of industrial polymer materials [5][6][7]. MALDI can generate important data on the telomer repeat units, end groups, and average molecular weights of these materials.…”

mentioning

confidence: 99%

The fragmentation of ethoxylated surfactants by AP-MALDI-QIT

Hanton

Parees

Zweigenbaum

2006

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has become an important technique to characterize the chemical structure of industrial polymer materials. MALDI methods have been developed to address a broad variety of different polymer materials containing different chemistries. One of the key aspects of the typical MALDI experiment is the generation of intact ions. The development of Atmospheric Pressure (AP) MALDI quadrupole ion trap (QIT) instruments has opened another channel to obtaining MS/MS experiments for polymer samples. These experiments provide a new method to obtain chemical structure information from MALDI experiments. Collision-Induced Dissociation (CID) provides an improved MALDI MS/MS experiment that can be done on readily available mass spectrometers. AP MALDI QIT techniques have been successfully applied to a variety of synthetic polymers. This work explores the applicability of AP MALDI QIT methods to relatively low molecular weight ethoxylated surfactants. In these experiments we show the CID fragmentation mass spectra on some ethoxylated surfactants, and demonstrate the existence of analyte matrix clusters. [5][6][7]. MALDI can generate important data on the telomer repeat units, end groups, and average molecular weights of these materials. MALDI methods have been developed to address a broad variety of different polymer materials containing different chemistries. One of the key aspects of the typical MALDI experiment is the generation of intact ions. MALDI mass spectra generally show little fragmentation. The production of intact ions is vital for the quantitation of average molecular weights, but limits the amount of chemical structural information that can be obtained from the data.The development of MALDI post-source decay (PSD) techniques has opened a new method to obtain chemical structure information from MALDI experiments [8 -10]. Unfortunately, PSD experiments are relatively crude MS/MS experiments. The range of masses submitted for the fragmentation is broad, the mass resolution and mass accuracy of the fragment spectra is not as good as the MALDI data, and there is very limited control of the energy deposition into the fragmentation process.The development of atmospheric pressure (AP) MALDI instruments has enabled the utilization of different mass analyzers for MALDI [11,12]., One of the key benefits to changing the mass analyzer in a MALDI experiment is to significantly improve the MS/MS capability. A quadrupole ion trap (QIT) mass spectrometer is an excellent choice in this regard. Some early work showed examples of high quality MS/MS experiments on MALDI generated peptides [13][14][15][16]. The ability to analyze synthetic polymers using AP MALDI methods on a QIT have also been demonstrated [17].With a QIT mass spectrometer we can accomplish high quality collision-induced dissociation (CID) MS/ MS. The instrument is capable of selecting an individual ion from the mass spectrum for fragmentation. The fragmentation gas and relative energy can be controlled...

show abstract

“…[18,21,19]). Such technique can provide the absolute molecular weight distribution of a number of molecules in the form of a spectrum: for each molecular weight, the amount of material having that molecular weight produces a peak having a certain intensity.…”

Section: Introductionmentioning

confidence: 99%

“…Non ionized fragments, on the contrary, do not appear in the spectrum. Such experiments may be performed by using several instrumental configurations, mainly triple quadrupole (QQQ), quadrupole time-of-flight (Q-TOF) and ion trap devices [19].…”

Section: Introductionmentioning

confidence: 99%

A Logic-Based Approach to Polymer Sequence Analysis

Bruni

2010

J Math Model Algor

View full text Add to dashboard Cite

Polymers are compounds formed by the joining of smaller, often repeating, units linked by covalent bonds. The analysis of their sequence is a fundamental issue in many areas of chemistry, medicine and biology. Nowadays, the prevalent approach to this problem consists in using a mass spectrometry analysis that gives information about the molecular weights of the polymer and of its fragments. This information should be used in order to obtain the sequence. This is however a difficult mathematical problem, and several approaches have been proposed for it. In particular, a promising one is based on a propositional logic modeling of the problem. This paper presents conceptual improvements in this approach, principally the off-line computation of a database that substantially speeds-up the sequencing operations. This is obtained by finding a correspondence between sequences and natural numbers, so that all sequences up to a certain molecular weight can be implicitly considered in the above database, and explicitly computed only when needed. Results on real-world problems show the effectiveness of this approach.

show abstract

Direct Mass Spectrometry of Polymers

Cited by 65 publications

References 35 publications

Extending the solvent-free MALDI sample preparation method

Extending the solvent-free MALDI sample preparation method

The fragmentation of ethoxylated surfactants by AP-MALDI-QIT

A Logic-Based Approach to Polymer Sequence Analysis

Contact Info

Product

Resources

About