Direct mass spectrometry approaches to characterize polyphenol composition of complex samples

Deconvolution of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry isotope patterns to determine ratios of A-type to B-type interflavan bonds in cranberry proanthocyanidins

“…However, the application of MALDI-TOF MS is usually qualitative because variability in analyte-matrix interactions on the MALDI target, difficulty in controlling desorption and ionization, and the non-linear detector responses of polydispersed oligomers create difficulties in developing quantitative methods (Fulcrand et al, 2008;Monagas et al, 2010;Stringano et al, 2011). However, the application described in this manuscript is not affected by these limitations because the distribution of A-to B-type interflavan bonds within each DP creates an isotope pattern that is a function of the natural abundance of C, H and O isotopes and ai.…”

Characterization of Proanthocyanidins from Parkia biglobosa (Jacq.) G. Don. (Fabaceae) by Flow Injection Analysis — Electrospray Ionization Ion Trap Tandem Mass Spectrometry and Liquid Chromatography/Electrospray Ionization Mass Spectrometry

“…Direct flow injection -electrospray ionization -mass spectrometry analysis can be used to establish the polyphenol profile of complex extracts [35], so technique was employed to obtain a preliminary qualitative metabolic fingerprint of the bark and root extracts of P. biglobosa, after a clean-up using LLE and SPE. The collected eluates were analyzed by ESI-IT-MS n both in positive and negative ionization mode.…”

“…Compounds isomeric with catechin dimers were detected for precursor ions at m/z 577 (8-11), 593 (12)(13)(14) and 609 (15)(16). Another series, with isomeric forms, were identified as esterified catechingallate and glucuronide dimers for ions at m/z 729 (17-18), 745 (19)(20)(21)(22), 761 (23)(24)(25)(26), 785 (27)(28), 881 (29)(30)(31), 897 (32)(33), 913 (34)(35) and 937 (36)(37). The retention time of these isomeric compounds were listed in Table 1.…”

Wine Metabolite Profiling: Possible Application in Winemaking and Grapevine Breading in Bulgaria

“…The GC-MS technology is less effective for analysis of polyphenols due to their low volatility, relatively high molecular weight and difficulties with the interpretation of the molecule fragmentation pattern from the experimental data. Thus, the metabolite profiling of wine polyphenols employs mainly liquid chromatography -mass spectrometry (LC-MS) as well as MALDI-MS and tandem MS/MS technologies, (12,13,16). Among them LC-MS is the most widely used, allowing characterization of complex structures of various grape and wine polyphenols, such as tannins, proanthocyanides, procyanides etc.…”