Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)

Otsuka, Yoichi

doi:10.5702/massspectrometry.a0095

Cited by 4 publications

(6 citation statements)

References 211 publications

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“…LESA has been used to characterise proteins in a wide array of samples, including dried blood spots, tissue sections, even living bacterial colonies. [10][11] 6,7,[12][13][14][15] To expand the analytical capabilities of the LESA technique, further improvements in spatial resolution are required. Another problem associated with the LESA technique is that the target molecules should be soluble to the solvents being used, and, as a result, the sampling efficiency for the insoluble compounds can become low.…”

Section: In Situ Samplingmentioning

confidence: 99%

Direct Analysis of Bio-Molecules in Solid Materials Using Electrospray Ionisation Mass Spectrometry Coupled with Laser Ablation and a Liquid Sampling Technique

2023

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In situ and rapid analysis of organic compounds using a combination of a newly-developed laser ablation in liquid (LAL) sampling technique combined with electrospray ionisation mass spectrometry (ESI-MS) is reported. The LAL is a technique that allows laser ablation to be conducted in a liquid medium containing organic compounds that were effectively extracted from solid materials into the liquid medium. Three organic compounds (valine, caffeine, and benzyl butyl phthalate (BBP)) were subjected to analysis. The LAL sampling was conducted in the fast-laser scanning mode using Galvanometric optics, and the total ablation time required for the sampling from a 1mm 2 area was about 3 sec, thus providing rapid sampling.The resulting sample solution was directly introduced into the ESI mass spectrometry system, without the need for any chromatographic separation. To evaluate the analytical capability of the LAL technique coupled with ESI-MS, both the overall transmission efficiencies of analytes from solid materials to the ion detector, and the repeatabilities of the measurements were rigorously tested. This involved the use of synthetic, in-house prepared standard materials containing the analytes. The overall ion yields were about 1.1x10 -3 % for valine, 8.7x10 -3 % for caffeine, and 6.7x10 -4 % for BBP. By comparing the ion yields obtained by the injection of an analyte solution and a standard solution through the mass spectrometer, the recoveries through the LAL sampling were approximately 31% for valine, 45% for caffeine and 37% for BBP. In addition, the analytical repeatabilities for all analytes were better than 6%. The analytical repeatabilities were mainly affected by either the heterogeneity of the in-house standard materials or changes in the plasma temperature by coexisting, laser-induced sample particles. It should be noted that not only water-soluble compounds (caffeine and valine), but also non-soluble compound (BBP) could be measured by the LAL-ESI-MS, which is one of the great advantages over the conventional liquid extraction surface analysis technique. The data obtained here clearly demonstrate that the LAL-ESI-MS has the potential for being a fast and user-friendly analytical technique for the in-situ detection for both the water-soluble and water-insoluble molecules.

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Section: In Situ Samplingmentioning

confidence: 99%

Direct Analysis of Bio-Molecules in Solid Materials Using Electrospray Ionisation Mass Spectrometry Coupled with Laser Ablation and a Liquid Sampling Technique

2023

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“…Unlike SIMS and MALDI, some atmospheric pressure sampling and ionization methods 9,10 have employed microextraction using a small volume of solvent in conjunction with electrospray ionization (ESI). 11 These methods are rapid and require minimal sample preparation.…”

Section: Introductionmentioning

confidence: 99%

“…7 Meanwhile, matrix assisted laser desorption/ionization (MALDI) can desorb and ionize components of a tissue by irradiating pulsed laser beam after applying a matrix coating. 8 Unlike SIMS and MALDI, some atmospheric pressure sampling and ionization methods 9,10 have employed microex-traction using a small volume of solvent in conjunction with electrospray ionization (ESI). 11 These methods are rapid and require minimal sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Solvent effects of N,N-dimethylformamide and methanol on mass spectrometry imaging by tapping-mode scanning probe electrospray ionization

Otsuka

One

Sun

et al. 2023

Analyst

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“…A variety of sampling/ ionization methods have been employed for MSI, such as secondary ion mass spectrometry, laser ablation electrospray ionization (LAESI), direct liquid extraction and ionization (DLEI), and matrix-assisted laser desorption/ionization (MALDI). 6,8 MALDI is a commercially available and commonly used soft ionization method. Unlike LC-MS-based analysis, which requires time-consuming and labor-intensive purification process, MALDI-MS-based analysis requires relatively simple sample preparation procedures due to its tolerance to relatively high level of salt and other contaminants.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Mass spectrometry imaging (MSI), with high molecular specificity, sensitivity, and throughput, has provided new dimensionality in the visualization of the metabolite distributions in tissues or cells. A variety of sampling/ionization methods have been employed for MSI, such as secondary ion mass spectrometry, laser ablation electrospray ionization (LAESI), direct liquid extraction and ionization (DLEI), and matrix-assisted laser desorption/ionization (MALDI). , …”

Section: Introductionmentioning

confidence: 99%

Nanosecond Photochemical Reaction for Enhanced Identification, Quantification, and Visualization of Primary Amine-Containing Metabolites by MALDI-Mass Spectrometry

Liu

et al. 2022

Anal. Chem.

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Many metabolites, including amino acids, neurotransmitters, and pharmaceuticals, contain primary amine functional groups. The analysis of these molecules by mass spectrometry (MS) plays an important role in the study of cancers and psychogenic diseases. However, the MS-based detection and visualization of these bioactive metabolites directly from real biological systems still suffer from challenges such as low ionization efficiency and/or matrix interference effects. Here, we introduce a simple and efficient strategy, the nanosecond photochemical reaction (nsPCR)-enabled fast chemical derivatization, enabling direct MS analysis of primary amine-containing metabolites, with enhanced detection sensitivity for numerous metabolites from cell culture medium and rat brain sections. Furthermore, this nsPCR-based chemical derivatization strategy was demonstrated to be a useful visualizing tool that could provide improved spatial information for these metabolites, potentially offering alternative tools for gaining novel insights into metabolic events.

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Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)

Cited by 4 publications

References 211 publications

Direct Analysis of Bio-Molecules in Solid Materials Using Electrospray Ionisation Mass Spectrometry Coupled with Laser Ablation and a Liquid Sampling Technique

Direct Analysis of Bio-Molecules in Solid Materials Using Electrospray Ionisation Mass Spectrometry Coupled with Laser Ablation and a Liquid Sampling Technique

Solvent effects of N,N-dimethylformamide and methanol on mass spectrometry imaging by tapping-mode scanning probe electrospray ionization

Nanosecond Photochemical Reaction for Enhanced Identification, Quantification, and Visualization of Primary Amine-Containing Metabolites by MALDI-Mass Spectrometry

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