Direct liquid chromatographic resolution of racemic drugs using α1-acid glycoprotein as the chiral stationary phase

Hermansson, Jörgen

doi:10.1016/s0021-9673(01)90787-3

Cited by 329 publications

(82 citation statements)

References 30 publications

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“…4 Other types of ISRP columns, several alkyl/diol silica (ADS), [5][6][7][8] dual zone 9 and mixed functional phase (MFP) types 10,11 had been developed. As a protein-immobilized stationary phase capable of plasma direct injection, octadecylsilane (ODS) treated with human plasma was developed and plasma direct injection capability was shown by Yoshida et al 12,13 α1-Acidglycoprotein (α1-AGP)-, ovomucoid-and avidin-coated columns had been developed, [14][15][16] and are capable of plasma direct injection. [17][18][19][20] Furthermore, direct analysis of drugs in serum using zwitterionic bile acid derivative-coated ODS was reported by Umemura et al 21 Several polymer-modified reversed-phase column materials such as the shielded hydrophobic phase (SHP) and the semipermeable surface (SPS) had also been reported.…”

Section: Introductionmentioning

confidence: 99%

Methylcellulose-immobilized Reversed-phase Precolumn for Direct Analysis of Drugs in Plasma by HPLC

et al. 2001

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We evaluated a new restricted access media (RAM) precolumn for direct analysis of drugs in plasma using a column switching HPLC system. The new RAM material was prepared by the modification of the external surface of porous silica with hydrophilic methylcellulose (MC), followed by modification of the internal surface with octadecylsilane (ODS). The external surface of the MC-immobilized ODS silica material (MC-ODS) suppressed the adsorption of proteins, while the internal surface of MC-ODS retained various types of drugs, such as ketoprofen, propranolol, caffeine and atenolol in plasma samples. In addition, MC-ODS allowed direct analysis of drugs in a 1000-µL plasma sample to monitor trace amounts of analytes contained. Reduced efficiency and clogging of the MC-ODS precolumn and/or the analytical column were not observed even after the repetitive injection of plasma sample up to 40 mL. Our results indicated that the MC-ODS precolumn could be used in pharmacodynamic and clinical studies.

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Section: Introductionmentioning

confidence: 99%

Methylcellulose-immobilized Reversed-phase Precolumn for Direct Analysis of Drugs in Plasma by HPLC

et al. 2001

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“…The above results indicate that the BSA-CSPs aminopropylsilica bonded BSA s-triazine is stable prepared with a linker of 2,4,6-tricholoro-1,3,5-triunder experimental conditions since the amino azine give more reliable and reproducible results. Table 3 9 Retention factors of the first eluted enantiomers (k ), separation factors (a), resolution factor (R ) and the eluents used for chromatography Furthermore, the synthesis method developed is very CSPs based on proteins have been used to resolve useful for the immobilization of biopolymers on the a wide number of chiral compounds of pharmacosolid media in situ, because the activated silica is logical interest [28,29] and to probe the binding of a relatively stable under normal conditions and the drug to proteins [30,31]. The effects of the various support can be prepared in 8 h.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers

Zhang

Zou

Wang

et al. 2000

Journal of Chromatography A

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“…These CSPs are prepared by using either a polymeric structure or a small ligand as chiral selector. The polymeric CSPs were classified in two categories: synthetic and naturally chiral polymers [2][3][4][5]. Cellulose and amylose are the most abundant natural polymers used in chiral separations.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric Separation and Quantitation of Tenofovir Disoproxil Fumarate Using Amylose-Based Chiral Stationary Phases by High-Performance Liquid Chromatography

Heydari¹,

Shamsipur²

2015

Acta Chromatographica

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Summary.A rapid high-performance liquid chromatography (HPLC) method for chiral purity determination of tenofovir disoproxil fumarate in raw material and pharmaceutical formulations was developed. The (S)-enantiomer appears to be as an impurity and pharmacologically inactive. The effects of various stationary phases, mobile phase composition, and column temperature on enantiomeric separation of tenofovir disoproxil were investigated and optimized. Chromatography resolution of tenofovir disoproxil enantiomers was performed on NUCLEOCEL ALPHA-RP S column (250 × 4.6 mm i.d., 5 µm). The elution was achieved by using 95:5% (v/v) methanol-acetonitrile, containing 0.1% triethylamine at a flow rate of 0.8 mL min −1 . The ultraviolet (UV) detector was set at 260 nm. Calibration curves were linear in the range of 1-100 µg mL −1 and 0.2-20 µg mL −1 for (R)-tenofovir disoproxil and (S)-enantiomer, respectively. Limits of detection and quantitation for (S)-enantiomer were 0.06 and 0.2 µg mL −1 . The run time of analysis was less than 7.0 min. The proposed method was used successfully for separation and quantification of tenofovir disoproxil enantiomers in raw material and pharmaceutical formulations.

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Direct liquid chromatographic resolution of racemic drugs using α1-acid glycoprotein as the chiral stationary phase

Cited by 329 publications

References 30 publications

Methylcellulose-immobilized Reversed-phase Precolumn for Direct Analysis of Drugs in Plasma by HPLC

Methylcellulose-immobilized Reversed-phase Precolumn for Direct Analysis of Drugs in Plasma by HPLC

Synthesis of a silica-bonded bovine serum albumin s-triazine chiral stationary phase for high-performance liquid chromatographic resolution of enantiomers

Enantiomeric Separation and Quantitation of Tenofovir Disoproxil Fumarate Using Amylose-Based Chiral Stationary Phases by High-Performance Liquid Chromatography

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