Direct Laser Writing for Deterministic Lateral Displacement of Submicron Particles

Alsharhan, Abdullah T.; Stair, Anthony J.; Acevedo, Rubén; Razaulla, Talha; Warren, Richard L.; Sochol, Ryan D.

doi:10.1109/jmems.2020.2998958

Cited by 10 publications

(11 citation statements)

References 35 publications

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“…DE drop-making devices have used either a 3D nested channel geometry and/or surface wetting control to achieve water-in-oil-in-water emulsification. ,− 3D geometries position a stream of water-in-oil droplets within a surrounding aqueous flow ensuring the correct formation of DEs, independent of the surface wetting of the channels. This concentric channel architecture is most commonly achieved by glass capillaries, − 3D printing, − or by multi-layer soft lithography. , These fabrication methods require complex equipment, expensive facilities, and/or technical expertise to perform. − Out of these, glass capillary devices feature greater compatibility with organic solvents and robust surface modification options, making them well suited to DE production for drug delivery and microparticle applications . However, glass capillaries are limited due to greater device-to-device variability and difficulty producing small DE drops due to the larger channel dimensions. − Smaller DE drops (∼50 um or less) are required for flow cytometry sorting and improve throughput during sorting, single-cell analysis, and ddPCR …”

Section: Resultsmentioning

confidence: 99%

Simplified, Shear Induced Generation of Double Emulsions for Robust Compartmentalization during Single Genome Analysis

Cowell

Dobria

Han

2022

ACS Appl. Mater. Interfaces

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Drop microfluidics has driven innovations for high throughput, low input analysis techniques such as single-cell RNA-seq. However, the instability of single emulsion (SE) drops occasionally causes significant merging during drop processing, limiting most applications to single-step reactions in drops. Here, we show that double emulsion (DE) drops address this critical limitation and completely prevent drop contents from mixing. DEs show excellent stability during thermal cycling. More importantly, DEs undergo rupture into the continuous phase instead of merging, preventing content mixing and eliminating unstable drops from the downstream analysis. Due to the lack of drop merging, the monodispersity of drops is maintained throughout a workflow, enabling the deterministic manipulation of drops downstream. We also developed a simple, one-layer DE drop maker compatible with simple surface treatment using a plasma cleaner. The device allows for the robust production of single-core DEs at a wide range of flow rates and better control over the shell thickness, both of which have been significant limitations of conventional two-layer devices. This approach makes the fabrication of DE devices much more accessible, facilitating its broader adoption. Finally, we show that DE droplets eliminate content mixing and maintain compartmentalization of single virus genomes during PCR-based amplification and barcoding, while SEs mixed contents due to merging. With their resistance to content mixing, DE drops have key advantages for multistep reactions in drops, which is limited in SEs due to merging and content mixing.

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Section: Resultsmentioning

confidence: 99%

Simplified, Shear Induced Generation of Double Emulsions for Robust Compartmentalization during Single Genome Analysis

Cowell

Dobria

Han

2022

ACS Appl. Mater. Interfaces

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“…The lateral displacement of the study noted 15.3 ± 8.6 µm over a 500 µm channel length. Results indicate the promising application of 3D-printed DLD systems for the manipulation of submicron particles [ 156 ] that can be used for efficient bioprinting of EVs.…”

Section: Evs In Bioengineering Applicationsmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Extracellular Vesicles for Therapeutic Use and in Bioengineering Applications

McLaughlin

Datta

Singh

et al. 2022

Cells

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Extracellular vesicles (EVs) are small lipid bilayer-delimited particles that are naturally released from cells into body fluids, and therefore can travel and convey regulatory functions in the distal parts of the body. EVs can transmit paracrine signaling by carrying over cytokines, chemokines, growth factors, interleukins (ILs), transcription factors, and nucleic acids such as DNA, mRNAs, microRNAs, piRNAs, lncRNAs, sn/snoRNAs, mtRNAs and circRNAs; these EVs travel to predecided destinations to perform their functions. While mesenchymal stem cells (MSCs) have been shown to improve healing and facilitate treatments of various diseases, the allogenic use of these cells is often accompanied by serious adverse effects after transplantation. MSC-produced EVs are less immunogenic and can serve as an alternative to cellular therapies by transmitting signaling or delivering biomaterials to diseased areas of the body. This review article is focused on understanding the properties of EVs derived from different types of MSCs and MSC–EV-based therapeutic options. The potential of modern technologies such as 3D bioprinting to advance EV-based therapies is also discussed.

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“…36,37 These fabrication methods, however, require complex equipment, expensive facilities, and/or technical expertise to perform. [38][39][40] Further, glass capillary fabrication is limited to larger channel dimensions and faces greater device-to-device variation, 41 which can make the production of the small DE drops that are suitable for flow cytometry sorting, 42 more challenging. To avoid full 3-D fabrication, semi-planar DE drop makers have been designed to use surface wetting to control emulsification.…”

Section: Introductionmentioning

confidence: 99%

Simplified, Shear Induced Generation of Double Emulsions for Robust Compartmentalization during Single Genome Analysis

Cowell

Dobria

Han

2021

Preprint

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Drop microfluidics has driven innovations for high throughput, low input analysis techniques such as single-cell RNA-seq. However, the instability of single emulsion (SE) drops occasionally causes significant merging during drop processing, limiting most applications to single-step reactions in drops. Here, we show that double emulsion (DE) drops address this critical limitation and completely prevent content mixing, which is essential for single entity analysis. DEs show excellent stability during thermal cycling. More importantly, DEs undergo rupture into the continuous phase instead of merging, preventing content mixing and eliminating unstable drops from the downstream analysis. Due to the lack of drop merging, the monodispersity of drops is maintained throughout a workflow, enabling the deterministic manipulation of drops downstream. We also developed a simple, one-layer fabrication method for DE drop makers. This design is powerful as it allows robust production of single-core DEs at a wide range of flow rates and better control over the shell thickness, both of which have been significant limitations of conventional two-layer devices. This approach makes the fabrication of DE devices much more accessible, facilitating its broader adoption. Finally, we show that DE droplets effectively maintain the compartmentalization of single virus genomes during PCR-based amplification and barcoding, while SEs mixed contents due to merging. With their resistance to content mixing, DE drops have key advantages for multistep reactions in drops, which is limited in SEs due to merging and content mixing.

show abstract

Direct Laser Writing for Deterministic Lateral Displacement of Submicron Particles

Cited by 10 publications

References 35 publications

Simplified, Shear Induced Generation of Double Emulsions for Robust Compartmentalization during Single Genome Analysis

Simplified, Shear Induced Generation of Double Emulsions for Robust Compartmentalization during Single Genome Analysis

Mesenchymal Stem Cell-Derived Extracellular Vesicles for Therapeutic Use and in Bioengineering Applications

Simplified, Shear Induced Generation of Double Emulsions for Robust Compartmentalization during Single Genome Analysis

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