Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient

Pellizzaro, Aline; Welker, Gabriel; Scott, D.K.; Solomon, Rance; Cooper, James P.; Farone, Anthony L.; Farone, Mary B.; Mushi, Robert; Aguinaga, Maria del Pilar; Erenso, Daniel

doi:10.1364/boe.3.002190

Cited by 31 publications

(42 citation statements)

References 17 publications

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“…Laser trapping has long been used to manipulate biological samples at the micron level [4,5]. However, to the best of our knowledge, it has never been used to study the ionization of a live cell in application of measuring the threshold radiation dose.…”

Section: Introductionmentioning

confidence: 99%

“…However, to the best of our knowledge, it has never been used to study the ionization of a live cell in application of measuring the threshold radiation dose. In our previous studies, we have used a high power laser trap to measure therapeutic efficacies in sickle cell anemia (SCA) disorder treatments [5]. During this process it was observed that a high power laser trap could ionize the human blood cells, effectively killing them, and eject them from the trap [6].…”

Section: Introductionmentioning

confidence: 99%

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Single cell ionization by a laser trap: a preliminary study in measuring radiation dose and charge in BT20 breast carcinoma cells

Kelley¹,

Gao²,

Erenso³

2016

Biomed. Opt. Express

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Abstract:In this work, a preliminary study in the application of a laser trap for ionization of living carcinoma cells is presented. The study was conducted using BT20 breast carcinoma cells cultured and harvested in our laboratory. Each cell, for a total of 50 cells, was trapped and ionized by a high intensity infrared laser at 1064 nm. The threshold radiation dose and the resultant charge from the ionization for each cell were determined. With the laser trap serving as a radiation source, the cell underwent dielectric breakdown of the membrane. When this process occurs, the cell becomes highly charged and its dielectric susceptibility changes. The charge creates an increasing electrostatic force while the changing dielectric susceptibility diminishes the strength of the trapping force. Consequently, at some instant of time the cell gets ejected from the trap. The time inside the trap while the cell is being ionized, the intensity of the radiation, and the post ionization trajectory of the cell were used to determine the threshold radiation dose and the charge for each cell. The measurement of the charge vs ionization radiation dose at single cell level could be useful in the accuracy of radiotherapy as the individual charges can collectively create a strong enough electrical interaction to cause dielectric breakdown in other cells in a tumor.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single cell ionization by a laser trap: a preliminary study in measuring radiation dose and charge in BT20 breast carcinoma cells

Kelley¹,

Gao²,

Erenso³

2016

Biomed. Opt. Express

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“…Since the first time a laser tweezer (LT) was introduced by Ashkin [1] over three decades ago, it has been widely used for the micromanipulation of living objects for both biological and biomedical applications [2][3][4][5][6][7], as well as for nonliving objects to construct specific microstructures for optoelectronic applications [8]. An LT is an intensity gradient trap formed by focusing a highly collimated laser beam used to manipulate dielectric objects as small as an atom and as large as 100 micrometers by creating small forces in the order of piconewtons.…”

Section: Introductionmentioning

confidence: 99%

“…For nucleic acids, LTs have been used to mechanically unzip several thousand basepairs of DNA sequences in vitro [4], and measure unfolding/refolding kinetics of RNA [5]. The use of LTs in studying RBCs has also lead to some biomedical applications as reported by studies that use LTs to measure efficacies of therapies used to treat diseases affecting the mechanical properties of RBCs, such as sickle cell anemia [6,7].…”

Section: Introductionmentioning

confidence: 99%

Application of a laser trap as a viscometer

et al. 2013

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A laser tweezer (LT) along with advanced imaging techniques has been widely applied to manipulate and study living as well as nonliving microscopic objects. In this study we present yet another novel application of LTs for a precise measurement of the viscosities of fluids in a micro-volume flow. We have demonstrated this novel application by measuring the viscosity of a fetal bovine serum (FBS) using a LT constructed from a single intensity gradient laser trap. By calibrating the LT using dielectric silica micro-beads in a fluid with a known viscosity, specifically water, and by suspending same size of silica beads in the FBS and trapping with the same trap, we have determined the viscosity of the FBS at different temperatures. We have used the relationship between the trapping and Stoke's drag force for a constant drag speed to determine the viscosity. We have also analyzed the viscosities determined in comparison with corresponding viscosities measured using an Ostwald viscometer.

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“…This technique is based on high intensity laser trapping of a single RBC. Laser trapping (LT) [2] techniques have been widely used to study the mechanical properties of RBCs [3,4]. In this study, we report our new procedure that demonstrates how individual RBCs can be charged, and the magnitude of the charge can be measured.…”

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confidence: 99%

Laser Trapping for Single Red Blood Cell (RBC) Ionization and Measurement of Charge

Cooper

Devito

Solomon

et al. 2014

Biomedical Optics 2014

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Application of high intensity gradient laser trap for charging a single cell is demonstrated. We used RBCs from normal person (AA hemoglobin) and an individual with sickle cell trait (AC hemoglobin). OCIS codes: (170.0170) Medical optics and biotechnology; (350.4855) Optical tweezers or optical manipulation IntroductionHigh performance liquid chromatography HPLC [1] is commonly used to determine the hemoglobin types present in a blood sample. Hemoglobin (Hb) quantitation in a blood sample is essential in screening sickle cell disease (SCD) and also in monitoring patients receiving various types of treatments. HPLC techniques employ principles of ion exchange chromatography and spectrophotometric detection. In this technique a few microliters of blood is hemolyzed and injected onto a positively charged column of HPLC. At a moderately alkaline pH, all hemoglobin cells carry a variable net negative charge and bind with the positive charge. However, the magnitude of the negative charge varies from one type of hemoglobin to another. Although there are many types of hemoglobin, the most common hemoglobin types found in blood are HbF, HbA, HbS, and HbC. In this order HbF has the weakest and HbC has the strongest negative charge. When these differentially negatively charged hemoglobin types are injected into the positively charged HPLC column, the HbF type will bind weakly and be eluted quickly from the column whereas the HbC type will bind more strongly and be retained longer on the column. Here we present a new technique that can be used to identify the different types of hemoglobin and the magnitude of the charge on the molecules. This technique is based on high intensity laser trapping of a single RBC. Laser trapping (LT) [2] techniques have been widely used to study the mechanical properties of RBCs [3,4]. In this study, we report our new procedure that demonstrates how individual RBCs can be charged, and the magnitude of the charge can be measured. We used a blood sample from an individual with sickle cell trait (SCT) and the AC hemoglobin type and a healthy subject with the AA hemoglobin type. Experimental MethodThe design and detailed discussion of the LT we used can be referenced in our recently reported studies on LT biomedical application [3]. Here we only discuss the basic elements of the experimental set-up that are needed to explain the procedure. The basic elements of the LT we used for this study are the laser, the microscope equipped with a high numerical aperture; a computer controlled digital camera, and a piezo-driven mechanical stage. The laser we used to form the trap is a linearly polarized infrared diode laser (5 watts at 1064nm) for which the power was controlled by a λ/2-wave plate and polarizer combination. After the laser beam was expanded and aligned, it was coupled to an inverted microscope (Olympus IX 71) and redirected for a normal incidence angle at the center of the back of an objective lens (OL) of the microscope using a dichroic mirror (DM) positioned at 45° inside the microscope to fo...

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Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient

Cited by 31 publications

References 17 publications

Single cell ionization by a laser trap: a preliminary study in measuring radiation dose and charge in BT20 breast carcinoma cells

Single cell ionization by a laser trap: a preliminary study in measuring radiation dose and charge in BT20 breast carcinoma cells

Application of a laser trap as a viscometer

Laser Trapping for Single Red Blood Cell (RBC) Ionization and Measurement of Charge

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