Direct laser fabrication of meso-scale 2D and 3D architectures with micrometric feature resolution

Accardo, Angelo; Courson, Rémi; Riesco, Roberto; Raimbault, Vincent; Malaquin, Laurent

doi:10.1016/j.addma.2018.04.027

Cited by 20 publications

(27 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, the main factors in choosing the construction process are accuracy, printing speed, material range, and dimensions of the desired object. According to the above considerations, the appropriate methods are microstereolithography (µSL) 5,6 , projection microstereolithography (PµSL) 7,8 , and two-photon polymerization (TPP) 9 .…”

Section: Introductionmentioning

confidence: 99%

A compact LED-based projection microstereolithography for producing 3D microstructures

Behroodi

Latifi

Najafi

2019

Sci Rep

View full text Add to dashboard Cite

Projection microstereolithography (PµSL) is a promising additive manufacturing technique due to its low cost, accuracy, speed, and also the diversity of the materials that it can use. Recently it has shown great potentials in various applications such as microfluidics, tissue engineering, micro-optics, biomedical microdevices, and so on. However, studies on PµSL are still ongoing in terms of the quality and accuracy of the construction process, which particularly affect the fabrication of complex 3D microstructures and make it attractive enough to be considered for commercial applications. In this paper, a compact LED-based PµSL 3D printer for the fabrication of 3D microstructures was developed, and the effective parameters that influence the quality of construction were thoroughly investigated and optimized. Accordingly, a customized optical system, including illumination optics and projection optics, was designed using optical engineering principles. This custom 3D printer was proposed for the PµSL process, which besides improving the quality of construction, led to the reduction of the size of the device, its cost-effectiveness, and the repeatability of its performance. To demonstrate the performance of the fabricated device, a variety of complex 3D microstructures such as porous, hollow, helical, and self-support microstructures were constructed. In addition, the repeatability of the device was assessed by fabricating microstructure arrays. The device performance showed that the lateral accuracy of printing was better than 5 μm, and the smallest thickness of the printed layer was 1 μm. Moreover, the maximum printable size of the device was 6.4 mm × 4 mm × 40 mm.

show abstract

Section: Introductionmentioning

confidence: 99%

A compact LED-based projection microstereolithography for producing 3D microstructures

Behroodi

Latifi

Najafi

2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Interestingly, we also demonstrated that sliding walls can be prepared by 3D printing, allowing for future versatile and low-cost implementation of walls with complex structures and functions libraries. The development of high-resolution 3D printing 34 will accompany the development of sliding wall technology and expand the range of its applications.…”

Section: Discussionmentioning

confidence: 99%

“…For this experiment, the sliding wall had a design similar to that used for valving with an integrated window (see Fig. 5a) and was produced with a high-resolution stereolithographic 3D printer 34 . A solution of polyethyleneglycol-diacrylate 700 (PEGDA) in Tris-borate-EDTA buffer (concentration 45% v/v) was photopolymerized inside the window, producing a hydrogel with a pore size of approximately 5 nm 35 , and was slid to the intersection with the fluidic channel linking reservoirs 1 and 2 (Fig.…”

Section: Membrane-based Electrokinetic Dna Preconcentration and Purifmentioning

confidence: 99%

Sliding walls: a new paradigm for fluidic actuation and protocol implementation in microfluidics

et al. 2020

Self Cite

View full text Add to dashboard Cite

Currently, fluidic control in microdevices is mainly achieved either by external pumps and valves, which are expensive and bulky, or by valves integrated in the chip. Numerous types of internal valves or actuation methods have been proposed, but they generally impose difficult compromises between performance and fabrication complexity. We propose here a new paradigm for actuation in microfluidic devices based on rigid or semi-rigid walls with transversal dimensions of hundreds of micrometres that are able to slide within a microfluidic chip and to intersect microchannels with hand-driven or translation stage-based actuation. With this new concept for reconfigurable microfluidics, the implementation of a wide range of functionalities was facilitated and allowed for no or limited dead volume, low cost and low footprint. We demonstrate here several fluidic operations, including on/off or switch valving, where channels are blocked or reconfigured depending on the sliding wall geometry. The valves sustain pressures up to 30 kPa. Pumping and reversible compartmentalisation of large microfluidic chambers were also demonstrated. This last possibility was applied to a "4D" migration assay of dendritic cells in a collagen gel. Finally, sliding walls containing a hydrogel-based membrane were developed and used to concentrate, purify and transport biomolecules from one channel to another, such functionality involving complex fluidic transport patterns not possible in earlier microfluidic devices. Overall, this toolbox is compatible with "soft lithography" technology, allowing easy implementation within usual fabrication workflows for polydimethylsiloxane chips. This new technology opens the route to a variety of microfluidic applications, with a focus on simple, hand-driven devices for point-of-care or biological laboratories with low or limited equipment and resources.

show abstract

“…A soft microcantilever (Bioplume V6, LAAS, Toulouse, France), a polymeric replication of a previously developed silicon microcantilever [39,40] fabricated by 3D-printing of a DS-3000 photoresist (DWS, Thiene, Italy) with a Dilase 3D printer (Kloe SA, St Mathieu de Tréviers, France) [41,42], was used to deposit microdrops of solutions on the microstructured face of the bundle. Details on the polymeric microcantilever conception are reported in the electronic Supplementary Materials ( Figure S4).…”

Section: Surface Biofunctionalizationmentioning

confidence: 99%

“…Previously used silicon cantilevers for optical fiber functionalization [35] did not allow a proper deposition of liquid drops onto the etched fibers used in this work as they damaged the higher aspect ratio and more fragile micropillar structures (See electronic Supplementary Materials Figure S5). Newly developed 3D-printed polymeric microcantilevers [41,42] (Figure 1 and electronic Supplementary Materials Figure S4) were soft enough to touch the apex without breaking the structures nor damaging the gold layer, and to deposit microdrops of solution with an appropriate size (see Figure 2A for the droplets' deposition plan (i), the schematic deposition principle (ii) and the images of the droplets deposition with the soft polymeric cantilever (iii, iv)). The spotting solutions were prepared with 5% glycerol in order to make them more viscous for liquid deposition and to avoid a complete spot drying.…”

Section: Surface Biofunctionalizationmentioning

confidence: 99%

Multiplexed Remote SPR Detection of Biological Interactions through Optical Fiber Bundles

Desmet

Vindas

Meza

et al. 2020

Sensors

Self Cite

View full text Add to dashboard Cite

The development of sensitive methods for in situ detection of biomarkers is a real challenge to bring medical diagnosis a step forward. The proof-of-concept of a remote multiplexed biomolecular interaction detection through a plasmonic optical fiber bundle is demonstrated here. The strategy relies on a fiber optic biosensor designed from a 300 µm diameter bundle composed of 6000 individual optical fibers. When appropriately etched and metallized, each optical fiber exhibits specific plasmonic properties. The surface plasmon resonance phenomenon occurring at the surface of each fiber enables to measure biomolecular interactions, through the changes of the retro-reflected light intensity due to light/plasmon coupling variations. The functionalization of the microstructured bundle by multiple protein probes was performed using new polymeric 3D-printed microcantilevers. Such soft cantilevers allow for immobilizing the probes in micro spots, without damaging the optical microstructures nor the gold layer. We show here the potential of this device to perform the multiplexed detection of two different antibodies with limits of detection down to a few tenths of nanomoles per liter. This tool, adapted for multiparametric, real-time, and label free monitoring is minimally invasive and could then provide a useful platform for in vivo targeted molecular analysis.

show abstract

Direct laser fabrication of meso-scale 2D and 3D architectures with micrometric feature resolution

Cited by 20 publications

References 31 publications

A compact LED-based projection microstereolithography for producing 3D microstructures

A compact LED-based projection microstereolithography for producing 3D microstructures

Sliding walls: a new paradigm for fluidic actuation and protocol implementation in microfluidics

Multiplexed Remote SPR Detection of Biological Interactions through Optical Fiber Bundles

Contact Info

Product

Resources

About