Direct laser fabrication of free-standing PEGDA-hydrogel scaffolds for neuronal cell growth

Accardo, Angelo; Blatché, Marie-Charline; Courson, Rémi; Loubinoux, Isabelle; Vieu, Christophe; Malaquin, Laurent

doi:10.1016/j.mattod.2018.02.004

Cited by 29 publications

(24 citation statements)

References 8 publications

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“…Axons use micro-topographical cues to sense and probe their immediate surroundings to trigger adaptive responses such as neuritic extension or retraction [10]. Such observations are clearly visible in 3D scaffolds developed using advanced lithography techniques [11,12], where neurites track along scaffold surfaces and change direction according to topography ( figure 3). In addition, extracellular proteins or lipid molecules found on the surface of adjacent cells embedded within hydrogels are also used as guidance cues [13].…”

Section: Physical Guidance Cues Within Hydrogel Systemsmentioning

confidence: 99%

Hydrogel systems and their role in neural tissue engineering

Madhusudanan

Raju

Shankarappa

2020

J. R. Soc. Interface.

131

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Neural tissue engineering (NTE) is a rapidly progressing field that promises to address several serious neurological conditions that are currently difficult to treat. Selecting the right scaffolding material to promote neural and non-neural cell differentiation as well as axonal growth is essential for the overall design strategy for NTE. Among the varieties of scaffolds, hydrogels have proved to be excellent candidates for culturing and differentiating cells of neural origin. Considering the intrinsic resistance of the nervous system against regeneration, hydrogels have been abundantly used in applications that involve the release of neurotrophic factors, antagonists of neural growth inhibitors and other neural growth-promoting agents. Recent developments in the field include the utilization of encapsulating hydrogels in neural cell therapy for providing localized trophic support and shielding neural cells from immune activity. In this review, we categorize and discuss the various hydrogel-based strategies that have been examined for neural-specific applications and also highlight their strengths and weaknesses. We also discuss future prospects and challenges ahead for the utilization of hydrogels in NTE.

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Section: Physical Guidance Cues Within Hydrogel Systemsmentioning

confidence: 99%

Hydrogel systems and their role in neural tissue engineering

Madhusudanan

Raju

Shankarappa

2020

J. R. Soc. Interface.

131

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“…diacrylate (PEG-DA) are applicable in tissue engineering creating 3D, hydrated, bio-mimetic structures for the encapsulation of living cells 36 . On the other hand, PEG-DA was demonstrated independently to be suitable with both technical implementations: DLP and NLL 37 . There exist photoresins typically used only for the high intensity (TW/cm 2 ) NLL-dedicated manufacturing of objects at nano-scale.…”

Section: Resultsmentioning

confidence: 99%

A Bio-Based Resin for a Multi-Scale Optical 3D Printing

Skliutas

Lebedevaite

Kašėtaitė

et al. 2020

Sci Rep

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Materials obtained from renewable sources are emerging to replace the starting materials of petroleumderived plastics. They offer easy processing, fulfill technological, functional and durability requirements at the same time ensuring increased bio-compatibility, recycling, and eventually lower cost. On the other hand, optical 3D printing (O3DP) is a rapid prototyping tool (and an additive manufacturing technique) being developed as a choice for efficient and low waste production method, yet currently associated with mainly petroleum-derived resins. Here we employ a single bio-based resin derived from soy beans, suitable for O3DP in the scales from nano-to macro-dimensions, which can be processed even without the addition of photoinitiator. The approach is validated using both state-of-the art laser nanolithography setup as well as a widespread table-top 3D printer-sub-micrometer accuracy 3D objects are fabricated reproducibly. Additionally, chess-like figures are made in an industrial line commercially delivering small batch production services. Such concept is believed to make a breakthrough in rapid prototyping by switching the focus of O3DP to bio-based resins instead of being restricted to conventional petroleum-derived photopolymers. Bio-based polymers are emerging as replacement for petroleum-derived polymers. The growth of the production and market is 2.05 Mtons of bio-plastics 1 and 700 bilion Euros in Europe only 2. The main advantages of bio-based plastic products compared to the conventional plastics are the preservation of fossil resources by using bio-mass which is a renewable resource and provision of the unique potential of carbon neutrality, as well as bio-degradability of the certain types of bio-plastics which offers additional means of recovery at the end of a product's life 3. The spectrum of bio-based plastics usage varies from nanocomposites 4-8 and films 9-11 to adsorbents 12-14. Vegetable oils are potential starting materials for the preparation of polymers due to their ready availability, inherent bio-degradability, negligible toxicity, and existence of modifiable functional groups 15. Nowadays there are a lot of scientific research dedicated to the light induced polymerization. As there exist diverse technical implementations of this technology, it is known in many names: lithography (stereolithography, digital light processing (DLP)/projection lithography), direct laser writing (DLW) or alternatively laser direct writing (LDW), two-photon polymerization (2PP), nonlinear lithography (NLL), multi-photon lithography (MPL), etc. However, this additive manufacturing process simply can be called by one common name: optical 3D printing (O3DP) as it is based on photons. This rapid prototyping tool is being developed as a choice for efficient and low waste production tool, yet currently associated with mainly petroleum-derived resins 16-19. On the other hand, O3DP in combination with post-processing (thermal-treatment) allows fabrication of free-form structures which can serve as 3D templates f...

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“…The two absorbed photons act as a single photon but with double the wavelength, and this phenomenon induces polymerization in a small region without disturbing other areas. The high resolution makes the 2PP technique suitable for biomedical applications [ 63 , 64 , 65 , 66 ]. Kufelt et al developed a methacrylate-modified hyaluronic acid (HAGM)-based scaffold for bone tissue engineering applications [ 67 ].…”

Section: Printing Methodsmentioning

confidence: 99%

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

Kim

Kong

Han

et al. 2020

IJMS

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The development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.

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Direct laser fabrication of free-standing PEGDA-hydrogel scaffolds for neuronal cell growth

Cited by 29 publications

References 8 publications

Hydrogel systems and their role in neural tissue engineering

Hydrogel systems and their role in neural tissue engineering

A Bio-Based Resin for a Multi-Scale Optical 3D Printing

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

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