Direct Involvement of Arachidonic Acid in the Development of Ear Edema via TRPV3

Sanaki, Takao; Kasai-Yamamoto, E.; Yoshioka, Takeshi; Sakaï, Shota; Yuyama, Ken‐ichi; Fujiwara, Takuji; Numata, Yoshito; Igarashi, Yasuyuki

doi:10.5650/jos.ess16227

Cited by 5 publications

(9 citation statements)

References 35 publications

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“…Skin levels of arachidonic acid (AA) metabolites, such as hydroxyeicosatetraenoic acids (HETEs), play important roles in the development of dermatitis [ 16 , 17 , 19 ]. Furthermore, niacin deficiency increases the levels of prostaglandin E synthase, which produces prostanoids, in the skin of mice and humans [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies showed that bacterially produced vitamin B6 [13,16], which is important for producing niacin, was insufficient to sustain the metabolism of the gut microbiota [11,12]. These studies reported that vitamin B6 deficiency reduced the relative abundance of Bacteroidaceae and increased that of Lachnospiraceae.…”

Section: Discussionmentioning

confidence: 99%

“…Harvested ears were immediately moved to liquid nitrogen, dried using a freeze dryer (VD-550R, Taitec, Kumagaya, Japan), and stored at −80°C until lipid extraction to investigate the tissue levels of prostaglandin E2, epoxyeicosatrienoic acid (EET), and dihydroxyeicosatrienoic acid in accordance with previous studies [ 16 , 17 ]. In some experiments, harvested ear samples were directly stored at −80°C.…”

Section: Methodsmentioning

confidence: 99%

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Analysis of the gut microbiome to validate a mouse model of pellagra

Susai

Kuroita

Kuronuma

et al. 2022

Bioscience of Microbiota, Food and Health

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Pellagra is caused by an abnormal intake and/or use of niacin, but its phenotypes are diverse. The phenotypes of pellagra can also be atypical, such as nausea. We previously reported a mouse model of pellagra-related nausea. However, the mechanism of this model is unclear. In this study, we found that the gut microbiota, which is thought to be a source of niacin, played an important role in the development of pellagra-related nausea in germ-free mice. We also investigated the gut microbiome. We compared urinary niacin metabolite levels and the dermal response between mice fed a normal diet and those fed a low-niacin diet to investigate the putative trigger of pellagra. Epoxyeicosatrienoic and hydroxyeicosatetraenoic acid levels were higher in mice fed a low-niacin diet compared with those fed a normal diet. Furthermore, histological studies indicated a dermatological response to the low-niacin diet. Interestingly, higher levels of oxidised fatty acids in response to the germ-free state were also observed. These findings indicate successful establishment of our newly established mouse model of pellagra via the gut microbiota.We believe that this model could enable the discovery of the putative cause of pellagra and phenotypes of pellagra that have not been recognised yet. INTRODUCTIONCPellagra is a clinical syndrome characterised by dermatitis, diarrhoea, dementia, and

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of the gut microbiome to validate a mouse model of pellagra

Susai

Kuroita

Kuronuma

et al. 2022

Bioscience of Microbiota, Food and Health

Self Cite

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“…These models can be used to identify compounds that interfere with AA metabolism and thus investigate the mechanisms of lipoxygenase (LOX) inhibition [38]. However, several studies have shown that AA-induced inflammation can be inhibited by mechanisms other than COX/LOX enzyme inhibition, such as the modulation of histamine and serotonin response [32,33,37,[39][40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Screening Anti-Inflammatory Effects of Flavanones Solutions

Bustos-Salgado

Andrade-Carrera

Domínguez-Villegas

et al. 2021

IJMS

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There are a large number of remedies in traditional medicine focused on relieving pain and inflammation. Flavanones have been a potential source in the search for leading compounds and biologically active components, and they have been the focus of much research and development in recent years. Eysenhardtia platycarpa is used in traditional medicine for the treatment of kidney diseases, bladder infections, and diabetes mellitus. Many compounds have been isolated from this plant, such as flavones, flavanones, phenolic compounds, triterpenoid acids, chalcones, sugars, and fatty acids, among others. In this paper, natural flavanone 1 (extracted from Eysenhardtia platycarpa) as lead compound and flavanones 1a–1d as its structural analogues were screened for anti-inflammatory activity using Molinspiration® and PASS Online in a computational study. The hydro alcoholic solutions (FS) of flavanones 1, 1a–1d (FS1, FS1a–FS1d) were also assayed to investigate their in vivo anti-inflammatory cutaneous effect using two experimental models, a rat ear edema induced by arachidonic acid (AA) and a mouse ear edema induced by 12-O-tetradecanoylphorbol acetate (TPA). Histological studies and analysis of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were also assessed in AA-inflamed rat ear tissue. The results showed that the flavanone hydro alcoholic solutions (FS) caused edema inhibition in both evaluated models. This study suggests that the evaluated flavanones will be effective when used in the future in skin pathologies with inflammation, with the results showing 1b and 1d to be the best.

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“…Based on all these results, it can be suggested that FS D has a similar activity profile to PLA2 inhibitors. On the other hand, the ear edema caused by topical application of AA has been widely used to evaluate COX and 5-LOX inhibitors [21]. Considering that BW755C is a dual COX/LOX inhibitor and zileuton is a 5-LOX inhibitor, they showed a higher anti-inflammatory activity against AA-induced than TPA-induced edema in previous studies [20].…”

Section: Discussionmentioning

confidence: 99%

Ex Vivo and In Vivo Anti-inflammatory Evaluations of Modulated Flavanones Solutions

Bustos-Salgado

Rodríguez-Lagunas

Domínguez-Villegas

et al. 2020

The 1st International Electronic Conference on Pharmaceutics

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Interest has developed in natural molecules due to their clinically proven effects on skin diseases. Flavanones display several biological activities, and recently have been the focus of studies due to their anti-inflammatory effect. To improve their pharmacological profile, four flavanones (A, B, C, and D) were synthesized by structural modification of one natural flavanone 1 (semi-systematic name: (2S)-5,7-dihydroxy-6-prenylflavanone) extracted from Eysenhardtia platycarpa. The hydroalcoholic flavanone solutions (FS) were assayed to investigate their anti-inflammatory effect on two in vivo cutaneous inflammation models. Materials and methods: the topical anti-inflammatory effects of FS were evaluated against models of 12-O-tetradecanoylphorbol acetate (TPA)-induced mouse ear edema and arachidonic acid (AA) in rat ear edema. Results: The vinylogous cyclized derivative (flavanone D) caused edema inhibition in the TPA-induced models with an inhibition of 96.27 ± 1.93%; equally effective and potent in inhibiting the mouse ear edema as indomethacin had been. In addition, the AA-induced increase in ear thickness was reduced the most by the topical application of modulated ether (flavanone B). Conclusions: The in vivo and histology results suggest that flavanones B and D are effective as topical anti-inflammatory agents in inflammatory processes. Thus, this new compound represents a promising agent for the management of skin diseases with an inflammatory component.

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Direct Involvement of Arachidonic Acid in the Development of Ear Edema via TRPV3

Cited by 5 publications

References 35 publications

Analysis of the gut microbiome to validate a mouse model of pellagra

Analysis of the gut microbiome to validate a mouse model of pellagra

Screening Anti-Inflammatory Effects of Flavanones Solutions

Ex Vivo and In Vivo Anti-inflammatory Evaluations of Modulated Flavanones Solutions

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