Direct Investigation of the Vectorization Properties of Amphiphilic Cyclodextrins in Phospholipid Films

Javierre, Isabelle; Nedyalkov, M.; Petkova, Valentina; Benattar, J. J.; Weisse, Sandrine; Auzély‐Velty, Rachel; Djedaïni‐Pilard, Florence; Perly, Bruno

doi:10.1006/jcis.2002.8545

Cited by 15 publications

(7 citation statements)

References 30 publications

(39 reference statements)

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“…By adding 30 mM ␤-cyclodextrin to the pull down during incubation, the amount of interacting proteins recovered was increased dramatically. Cyclodextrins have been shown to aid in protein folding and lipid sequestration (35,44). The reason that ␤-cyclodextrin enhances the protein-protein interactions could be that the cyclodextrin sequesters lipids from the solubilized membranes and removes any barrier for the proteins to interact or allows the proteins to maintain an appropriate conformation for interaction.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Phosphatidylinositol Phosphate Kinase 1 Binds F-actin and Recruits Phosphatidylinositol 4-Kinase β1 to the Actin Cytoskeleton

Davis

Im²,

Dubin³

et al. 2007

Journal of Biological Chemistry

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The actin cytoskeleton can be influenced by phospholipids and lipid-modifying enzymes. In animals the phosphatidylinositol phosphate kinases (PIPKs) are associated with the cytoskeleton through a scaffold of proteins; however, in plants such an interaction was not clear. Our approach was to determine which of the plant PIPKs interact with actin and determine whether the PIPK-actin interaction is direct. Our results indicate that AtPIPK1 interacts directly with actin and that the binding is mediated through a predicted linker region in the lipid kinase. AtPIPK1 also recruits AtPI4K␤1 to the cytoskeleton. Recruitment of AtPI4K␤1 to F-actin was dependent on the C-terminal catalytic domain of phosphatidylinositol-4-phosphate 5-kinase but did not require the presence of the N-terminal 251 amino acids, which includes 7 putative membrane occupation and recognition nexus motifs. In vivo studies confirm the interaction of plant lipid kinases with the cytoskeleton and suggest a role for actin in targeting PIPKs to the membrane. Phosphatidylinositol phosphate kinases (PIPKs)2 are a family of enzymes that phosphorylate phosphatidylinositol phosphates (PtdInsP) to phosphatidylinositol bisphosphates (PtdInsP 2 ). Arabidopsis has 11 predicted isoforms of PtdInsP kinases (1). AtPIPK1 and AtPIPK10 have been characterized biochemically, and PtdIns4P is the primary substrate making the predominant product phosphatidylinositol (4, 5) bisphosphate (PtdIns(4,5)P 2 ), suggesting that they are similar to the type I PIPKs found in humans (2-5). However, unlike the mammalian, yeast or Caenorhabditis elegans PIPKs, AtPIPK1-9 contain N-terminal putative membrane occupation and recognition nexus (MORN) motifs similar to those first reported in junctophilins (1, 6). MORN motifs are unique to this family of enzymes and have not been reported in any other eukaryotic lipid kinases.In eukaryotic models PtdInsP kinases supply PtdIns(4,5)P 2 for many cellular functions, and it is well known that phospholipids play an integral role in regulating the structure and dynamics of the cytoskeleton through the many actin-binding proteins that interact with PtdIns(4,5)P 2 (7-10). For example, the absence of PtdInsP kinases in yeast has an adverse affect on the yeast cell morphology (11) and causes them to be unable to properly form actin cables (12). In some animal cell lines overexpression of PtdInsP kinases results in the formation of actin comet tails and stress fibers (13, 14) and disruption of membrane trafficking (14). In these animal cell lines, a functional PtdInsP kinase producing PtdIns(4,5)P 2 was necessary to cause the changes in cytoskeletal structure (13,15). In mammalian systems, PIPK activity has been recovered with an F-actin fraction, and the ␣, ␤, and ␥ isoforms have been identified as copurifying with F-actin. The data support a model where these PIPKs do not directly interact with F-actin but that this interaction is mediated by the presence of Racs, small GTP-binding proteins (16). In plants, PtdIns kinase activity (17, 18) and PtdIns...

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Section: Discussionmentioning

confidence: 99%

Arabidopsis Phosphatidylinositol Phosphate Kinase 1 Binds F-actin and Recruits Phosphatidylinositol 4-Kinase β1 to the Actin Cytoskeleton

Davis

Im²,

Dubin³

et al. 2007

Journal of Biological Chemistry

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“…The fact that modified cyclodextrins can be mixed in various proportions with phospholipids, cholesterol, and other amphiphilic molecules has been exploited for preparation of functionalized lipid membranes and organized biomimetic systems ( Table 2 ). Hydrated mixtures of lipids and amphiphilic cyclodextrins may spontaneously assemble into unilamellar spherical vesicles ( Figure 7 a), giant unilamellar vesicles ( Figure 7 b) or multilamellar liposomes [ 28 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ]. More sophisticated hybrid structures, involving cyclodextrin derivatives, have been prepared by controlled deposition techniques employing nanoarchitectonics means [ 7 , 14 , 15 ].…”

Section: Nanosystems Of Amphiphilic Cyclodextrins Mixed With Membrmentioning

confidence: 99%

“…In general, high hydrophobicity of the cyclodextrin derivatives might constitute an obstacle for their inclusion in lipid bilayer membranes owing to the preference for phase separation in two-dimensional domains. A variety of physical and physico-chemical methods (small-angle X-ray scattering (SAXS), X-ray diffraction, small-angle neutron scattering (SANS), X-ray-reflectivity, neutron reflectivity, differential scanning calorimetry (DSC), deuterium nuclear magnetic resonance ( 2 H NMR), surface pressure/area isotherms, Brewster angle microscopy (BAM), atomic force microscopy (AFM), cryogenic transmission electron microscopy (cryo-TEM), and confocal laser scanning microscopy (CLSM)) have been employed to investigate these effects [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 …”

Section: Nanosystems Of Amphiphilic Cyclodextrins Mixed With Membrmentioning

confidence: 99%

“…An important feature for the drug delivery field comes from the fact that the majority of amphiphilic cyclodextrins are considered to be non-hemolytic and non-cytotoxic [ 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 ]. Mixed amphiphilic nanostructures of phospholipids and modified cyclodextrin have been of special interest because these systems may display high affinity for interaction with biological membranes [ 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ].…”

Section: Introductionmentioning

confidence: 99%

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Nano-Assemblies of Modified Cyclodextrins and Their Complexes with Guest Molecules: Incorporation in Nanostructured Membranes and Amphiphile Nanoarchitectonics Design

2014

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A variety of cyclodextrin-based molecular structures, with substitutions of either primary or secondary faces of the natural oligosaccharide macrocycles of α-, β-, or γ-cyclodextrins, have been designed towards innovative applications of self-assembled cyclodextrin nanomaterials. Amphiphilic cyclodextrins have been obtained by chemical or enzymatic modifications of their macrocycles using phospholipidyl, peptidolipidyl, cholesteryl, and oligo(ethylene oxide) anchors as well as variable numbers of grafted hydrophobic hydrocarbon or fluorinated chains. These novel compounds may self-assemble in an aqueous medium into different types of supramolecular nanoassemblies (vesicles, micelles, nanorods, nanospheres, and other kinds of nanoparticles and liquid crystalline structures). This review discusses the supramolecular nanoarchitectures, which can be formed by amphiphilic cyclodextrin derivatives in mixtures with other molecules (phospholipids, surfactants, and olygonucleotides). Biomedical applications are foreseen for nanoencapsulation of drug molecules in the hydrophobic interchain volumes and nanocavities of the amphiphilic cyclodextrins (serving as drug carriers or pharmaceutical excipients), anticancer phototherapy, gene delivery, as well as for protection of instable active ingredients through inclusion complexation in nanostructured media.

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“…Phospholipid NBFs are a good model for studying some aspects of biological membranes since they involve the same basic interactions. In addition, l -α-phosphatidylcholine dimyristoyl (DMPC) films were already used as targets to investigate the insertion of modified cyclodextrins, covalently bound to a cholesterol tail, within a membrane …”

Section: Introductionmentioning

confidence: 99%

Hydration of Black Foam Films Made of Amphiphilic Cyclodextrins

et al. 2004

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We investigate the interfacial behavior of a new type of amphiphilic cyclic oligosaccharide obtained by grafting a phospholipid onto a methylated cyclodextrin. These compounds are able to form stable black foam films, the structure of which can be determined using X-ray reflectivity. These films consist of a highly hydrated bilayer of modified cyclodextrins which are remarkably thick due to their abundant hydration core. In the present paper, we explore the influence of cyclodextrin hydration on the film structure. The hydration rate of the films is tuned by changing the cyclodextrin methylation, by binding the modified cyclodextrins to various molecules (complex formation) and by exposing the films to IR radiation. In addition, we show that the gas permeability of these phospholipidyl−cyclodextrin films is governed by their central layer of bound water, which is an efficient barrier against gas permeation.

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Direct Investigation of the Vectorization Properties of Amphiphilic Cyclodextrins in Phospholipid Films

Cited by 15 publications

References 30 publications

Arabidopsis Phosphatidylinositol Phosphate Kinase 1 Binds F-actin and Recruits Phosphatidylinositol 4-Kinase β1 to the Actin Cytoskeleton

Arabidopsis Phosphatidylinositol Phosphate Kinase 1 Binds F-actin and Recruits Phosphatidylinositol 4-Kinase β1 to the Actin Cytoskeleton

Nano-Assemblies of Modified Cyclodextrins and Their Complexes with Guest Molecules: Incorporation in Nanostructured Membranes and Amphiphile Nanoarchitectonics Design

Hydration of Black Foam Films Made of Amphiphilic Cyclodextrins

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