Direct interface of chemistry to microbiological systems: membrane inlet mass spectrometry

Lloyd, David; Thomas, Kerrie L.; Cowie, G. A.; Tammam, Jonathan; Williams, Alan G.

doi:10.1016/s0167-7012(01)00331-1

Cited by 29 publications

(15 citation statements)

References 47 publications

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“…Whereas in environmental research MIMS is very successfully applied in soil science or in studies on microbial turnover processes (e.g. [8][9][10][11][12][13]), it is a rather new application in marine and limnic research. Studies by Tortell [14] of 02' DMS or N 2 concentrations in surface waters or by Hartnett and Seitzinger [15], investigating nitrogen concentrations in sediment cores, underlined the suitability of MIMS for geochemical investigations of marine and limnic environments.…”

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Application of membrane inlet mass spectrometry for online and in situ analysis of methane in aquatic environments

Schlüter

Gentz

2008

J. Am. Soc. Mass Spectrom.

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A method is presented for the online measurement of methane in aquatic environments by application of membrane inlet mass spectrometry (MIMS). For this purpose, the underwater mass spectrometer Inspectr200-200 was applied. A simple and reliable volumetric calibration technique, based on the mixing of two end member concentrations, was used for the analysis of CH 4 by MIMS. To minimize interferences caused by the high water vapor content, permeating through the membrane inlet system into the vacuum section of the mass spectrometer, a cool-trap was designed. With the application of the cool-trap, the detection limit was lowered from 100 to 16 nmol/L CH 4 . This allows for measurements of methane concentrations in surface and bottom waters of coastal areas and lakes. Furthermore, in case of membrane rupture, the cool-trap acts as a security system, avoiding total damage of the mass spectrometer by flushing it with water. The Inspectr200-200 was applied for studies of methane and carbon dioxide concentrations in coastal areas of the Baltic Sea and Lake Constance. The low detection limit and fast response time of the MIMS allowed a detailed investigation of methane concentrations in the vicinity of gas seepages. aAm Soc Mass T he analysis of methane as well as other trace gases like carbon dioxide, nitrous oxide, or dimethyl sulphide in aquatic environments is a major objective of basic and applied research. This includes investigations of the air-sea exchange of these greenhouse gases as well as of the release of methane from gassy sediments, hydrocarbon reservoirs, and pipelines, or through dissociation of gas hydrates. Although the spatial extent of such discharge sites is often rather small, ranging from a few square meters to several square kilometers, they are considered major drivers for the marine methane cycle in the present and past [1][2][3][4].The localization of such discharge sites as well as the detection and quantification of trace gases in lakes or the ocean relies essentially on water sampling (e.g., by Rosette Water Samplers, Hydro-Bios, Kiel, Germany) and subsequent chemical analysis by gas chromatography (GC) or infrared-spectrometry (IR) onboard the research vessel. Application of GC as well as IR requires the phase transfer of gases from the dissolved to the gaseous phase. For this purpose, head-space techniques, vacuum-degassing, or spray chambers are applied [5].Head-space techniques and vacuum-degassing are very suitable for analysis of discrete water samples but or N 2 0 in surface waters sampled along transects by research vessels. Nevertheless, the time required for equilibration and subsequent GC or IR analysis is about several tens of minutes to hours [7]. Hence, highresolution investigations, in time and space, of trace gases at natural gas seepages like pockmarks (morphological depressions at the seafloor) or hydrocarbon leakages during surveys by research vessels are rather difficult and time consuming.Compared with such rather long measuring times, application of membrane inlet mass...

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confidence: 99%

Application of membrane inlet mass spectrometry for online and in situ analysis of methane in aquatic environments

Schlüter

Gentz

2008

J. Am. Soc. Mass Spectrom.

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“…This strategy can be expanded to provide for greater membrane selectivity based upon specific enzyme-substrate affinities. There are a number of potential applications for enzyme modified MIMS interfaces that may arise from the enzymological and biological work reviewed by Lloyd et al [12]. We have not yet explored potential complications arising from the analysis of mixtures of esters, such as competitive inhibition and multiple esters producing the same hydrolysis product.…”

Section: Discussionmentioning

confidence: 99%

“…The inherent sensitivity and selectivity of mass spectrometry (especially tandem MS techniques) combined with the use of semipermeable membranes to select for compounds of interest in raw, complex sample matrices makes MIMS ideal for monitoring dynamic chemical systems. The simplicity and speed of this system makes it well suited for gathering online data from reacting chemical streams, bioreactors, and other biological systems as well as rapid screening of environmental samples and pharmaceutical products [1,2,4,12].…”

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confidence: 99%

“…A variety of MIMS interface geometries and materials have been successfully employed including functionalized [13], ultra-thin [14], flat sheet [15], a n d hollow fiber [2,12,16,17] membranes. In particular, capillary hollow fiber membranes (CHFMs) made of polydimethylsiloxane (PDMS) and arranged in a flow over configuration with pneumatically assisted carrier gas flowing through the lumen of the capillary (as presented by this work) have gained popularity [1,2].…”

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An enzyme derivatized polydimethylsiloxane (PDMS) membrane for use in membrane introduction mass spectrometry (MIMS)

Creba

Weissfloch

Krogh

et al. 2007

J. Am. Soc. Mass Spectrom.

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Membrane introduction mass spectrometry (MIMS) provides direct measurement of volatile and semivolatile analytes in condensed and gas-phase samples without sample preparation steps. Although MIMS has numerous advantages that include direct, on-line, real-time analysis with low detection limits, current applications of MIMS are predominantly limited to volatile and semivolatile analytes that permeate hydrophobic membranes (e.g., polydimethylsiloxane; PDMS). We report the first enzyme modified PDMS membrane for use with MIMS. This was achieved by immobilizing Candida rugosa lipase directly onto the surface of oxidized PDMS. These surface immobilized enzymes catalyze ester hydrolysis, releasing an alcohol product at the membrane interface that is readily detected. We have successfully used an enzyme modified membrane for the analysis and quantification of low-volatility and hydrophilic esters. We report the quantification of several carboxylic acid esters in dilute aqueous solutions, including a phthalate monoester carboxylate that is not readily detected by conventional MIMS. This new interface demonstrates the potential for extending the range and versatility of MIMS.

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“…For instance, subsurface methane profiles and gas emissions from wetlands and soils are monitored directly by mass spectrometry probes (Lloyd et al 2002;Beckmann et al 2004), and photosynthetic activities measured with high spatial and temporal resolution. Performed at various levels in the tropical canopy, studies for over several months using arrays of small light sensors enabled the conclusion that actual photosynthesis of tropical epiphytes was determined by the specific and fluctuating light conditions of their microhabitat and cannot be ascribed to light-adapted ancestors (Rascher et al 2012).…”

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confidence: 99%

Alfred Russel Wallace: Self-Educated Genius and Polymath

Lloyd

2014

Progress in Botany

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Direct interface of chemistry to microbiological systems: membrane inlet mass spectrometry

Cited by 29 publications

References 47 publications

Application of membrane inlet mass spectrometry for online and in situ analysis of methane in aquatic environments

Application of membrane inlet mass spectrometry for online and in situ analysis of methane in aquatic environments

An enzyme derivatized polydimethylsiloxane (PDMS) membrane for use in membrane introduction mass spectrometry (MIMS)

Alfred Russel Wallace: Self-Educated Genius and Polymath

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