Direct interaction of hydrophilic gold nanoparticles with dexamethasone drug: Loading and release study

Venditti, Iole; Fontana, Laura; Fratoddi, Ilaria; Battocchio, Chiara; Cametti, C.; Sennato, Simona; Mura, Francesco; Sciubba, Fabio; Delfini, Maurizio; Russo, Maria Vittoria

doi:10.1016/j.jcis.2013.11.063

Cited by 58 publications

(41 citation statements)

References 46 publications

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“…A number of facets of these techniques have been presented and discussed over the past years in a series of papers from our group [45][46][47][48][49][50][51][52][53][54][55][56].…”

Section: Gold Nanospheresmentioning

confidence: 99%

How toxic are gold nanoparticles? The state-of-the-art

et al. 2015

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With the growing interest in the applications of gold nanoparticles in biotechnology and their physiological effects, possible toxicity of gold nanoparticles is becoming an increasingly important issue. A large number of studies carried out over the past few years under a variety of experimental conditions and following different protocols have produced conflicting results, leading to divergent views about the actual safety of gold nanoparticles in human applications.This work is intended to provide an overview of the most recent experimental results and thereby summarize current state-of-the-art. Rather than presenting a comprehensive review of the available literature in this field, which would be impractically broad, we have selected representative examples of both in vivo and in vitro studies, which clearly demonstrate the need for urgent and rigorous standardization of experimental protocols. Despite their significant potential, the safety of gold nanoparticles is highly controversial at this time, and important concerns have been raised that need to be properly addressed. Factors such as shape, size, surface charge, coating, and surface functionalization are expected to influence the interactions of particles with biological systems to a different extent, resulting in different outcomes and influencing the potential of gold nanoparticles for biomedical applications.Moreover, despite continuous attempts to establish a correlation between structure of the particles and their interactions with biological systems, we are still far from elucidating the toxicological profile of gold nanoparticles in an indisputable manner. This review is intended to contribute towards this goal, offering a number of suggestions on how to achieve the systematization of data on the most relevant physico-chemical parameters, which govern and control the toxicity of gold nanoparticles at cellular and whole-organism levels.

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“…A number of facets of these techniques have been presented and discussed over the past years in a series of papers from our group [45][46][47][48][49][50][51][52][53][54][55][56].…”

Section: Gold Nanospheresmentioning

confidence: 99%

How toxic are gold nanoparticles? The state-of-the-art

et al. 2015

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“…Bioconjugation was carried out following the non-covalent procedure already assessed in the literature [51], for similar compounds either on the 1/4 and 1/10 Au/S Au-3MPS samples [52,53]. In order to assess the loading, a calibration curve was done for MTX in H 2 O (pH 5.…”

Section: Loading Of Mtx On Au-3mpsmentioning

confidence: 99%

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

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Venditti

Benassi

et al. 2016

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tGold nanoparticles (AuNPs) represent an effective choice for topical drug delivery systems thanks to their small size, general non-toxicity, ease of functionalization and high surface to volume ratio. Even if systemic, methotrexate still plays an important role in psoriasis treatment: its topical use shows insufficient percutaneus penetration owing to limited passive diffusion, high molecular weight and dissociation at physiological pH. The aim of our study was to design a new drug delivery nanocarrier for Methotrexate and to improve its solubility, stability and biodistribution. AuNPs were on purpose prepared with a hydrophilic stabilizing layer, in order to improve the colloidal stability in water. Watersoluble gold nanoparticles functionalized by sodium 3-mercapto-1-propansulfonate (Au-3MPS) were prepared and loaded with methotrexate (MTX). The loading efficiency of MTX on Au-3MPS was assessed in the range 70-80%, with a fast release (80% in one hour). The release was studied up to 24 h reaching the value of 95%. The Au-3MPS@MTX conjugate was fully characterized by spectroscopic techniques (UV-vis, FTIR) and DLS. Preliminary toxicity tests in the presence of keratinocytes monolayers allowed to assess that the used Au-3MPS are not toxic. The conjugate was then topically used on C57BL/6 mouse normal skin in order to trace the absorption behavior. STEM images clearly revealed the distribution of gold nanoparticles inside the cells. In vitro studies showed that Methotrexate conjugated with Au-3MPS is much more efficient than Methotrexate alone. Moreover, DL50, based on MTT analysis, is 20 folds reduced at 48 h, by the presence of nanoparticles conjugation. UV-vis spectra for in vivo tracing of the conjugate on bare mouse skin after 24 h of application, show increased delivery of Methotrexate in the epidermis and dermis using Au-3MPS@MTX conjugate, compared to MTX alone. Moreover we observed absence of the Au-3MPS in the dermis and in the epidermis, suggesting that these layers of the skin do not retain the nanoparticles. Based on our data, we found that the novel Au-3MPS@MTX conjugate is an effective non-toxic carrier for the satisfactory percutaneous absorption of Methotrexate and could help in possible topical treatment of psoriasis.

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“…The functionalisation of the nanoparticles in solution are mainly used for solid-liquid heterogeneous catalysis [60][61][62]. Others studies are reported on the immobilisation of enzyme on the gold nanoparticles by the stabilisation of these NPs with π-conjugated dithiol ligands for biocatalytic applications [63][64][65][66][67].…”

Section: Introductionmentioning

confidence: 99%

Dynamic Processes on Gold-Based Catalysts Followed by Environmental Microscopies

et al. 2017

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Abstract:Since the early discovery of the catalytic activity of gold at low temperature, there has been a growing interest in Au and Au-based catalysis for a new class of applications. The complexity of the catalysts currently used ranges from single crystal to 3D structured materials. To improve the efficiency of such catalysts, a better understanding of the catalytic process is required, from both the kinetic and material viewpoints. The understanding of such processes can be achieved using environmental imaging techniques allowing the observation of catalytic processes under reaction conditions, so as to study the systems in conditions as close as possible to industrial conditions. This review focuses on the description of catalytic processes occurring on Au-based catalysts with selected in situ imaging techniques, i.e., PEEM/LEEM, FIM/FEM and E-TEM, allowing a wide range of pressure and material complexity to be covered. These techniques, among others, are applied to unravel the presence of spatiotemporal behaviours, study mass transport and phase separation, determine activation energies of elementary steps, observe the morphological changes of supported nanoparticles, and finally correlate the surface composition with the catalytic reactivity.

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Direct interaction of hydrophilic gold nanoparticles with dexamethasone drug: Loading and release study

Cited by 58 publications

References 46 publications

How toxic are gold nanoparticles? The state-of-the-art

How toxic are gold nanoparticles? The state-of-the-art

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Dynamic Processes on Gold-Based Catalysts Followed by Environmental Microscopies

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