Direct injection HPLC method for the determination of phenylbutazone and oxyphenylbutazone in serum using a semipermeable surface column

Haque, Ahsanul; Stewart, James T.

doi:10.1016/s0731-7085(97)00041-1

Cited by 11 publications

(3 citation statements)

References 16 publications

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“…Indeed, up to 50 ml of plasma can be loaded on this extraction sorbent [92]. SPS material has been successfully applied to the analysis of small molecules in biological fluids [38,43,[92][93][94][95][96][97][98][99][100][101][102][103][104].…”

Section: Porous Silica Covered By a Combined Ligandmentioning

confidence: 99%

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

Souverain

Rudaz

Veuthey

2004

Journal of Chromatography B

247

124

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Section: Porous Silica Covered By a Combined Ligandmentioning

confidence: 99%

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

Souverain

Rudaz

Veuthey

2004

Journal of Chromatography B

247

124

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“…A high performance liquid chromatography (HPLC) method was developed by Taylor and Westwood (1995) to measure PBZ and OPBZ levels in the plasma of race horses; the method had a limit of quantitation (LOQ) of less than 1 μg mL −1 . Another HPLC screening method with confirmation by gas chromatography-mass spectrometry (Neto, Andraus, & Salvadori, 1996) had a similar LOQ (1 μg ml −1 ) for plasma and urine and HPLC was also used to detect PBZ and OPBZ in serum (Haque & Stewart, 1997) with a LOQ of 0.5 μg mL −1 . Liquid chromatography with UV detection was employed by De Veau (1999) to quantify PBZ in bovine plasma at concentrations within the range of 20 ng to 2 μg mL −1 and PBZ was included in a qualitative multi-residue method to detect 6 NSAIDs in bovine muscle by liquid chromatography-tandem mass spectrometry (Van Hoof, De Wasch, Poelmans, Noppe, & De Brabander, 2004); the method could detect the analyte at 100 μg kg −1 .…”

Section: Introductionmentioning

confidence: 99%

Production of antisera to phenylbutazone and oxyphenylbutazone for use in immunochemical detection assays

Fodey

Barnes

Crooks

2015

Food and Agricultural Immunology

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Phenylbutazone, a non-steroidal anti-inflammatory drug commonly used to treat horses for musculoskeletal disorders, can cause adverse effects in humans and is therefore not permitted for use in food-producing animals in the EU. In recent years some equine and bovine samples have been found to be non-compliant. While physicochemical methods of analysis exist there is a need for lessexpensive, rapid screening methods such as immunoassays. This study describes the production of antisera to detect phenylbutazone and its main metabolite, oxyphenylbutazone. Four haptens were chosen for immunogen preparation and two of these, suxibuzone and γ-hydroxyphenylbutazone, produced antisera with 50% of maximal inhibitory concentration (IC 50 s) of <5 ng mL −1 for phenylbutazone. Antisera with IC 50 s of 5.5 ng mL −1 for phenylbutazone and 5.6 ng mL −1 for oxyphenylbutazone were produced by their respective haptens. The figures suggest that the antisera could deliver detection capabilities below the concentration that is recommended by the Community Reference Laboratory (5 ng mL −1 ).

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“…The most common side effects of PBZ involves the gastrointestinal system. For the determination of PBZ, several analytical methods were reported, including high-performance liquid chromatography, [14][15][16][17] Direct injection HPLC method, 18,19 Proton magnetic resonance spectroscopic, 20,21 and gas chromatography-mass spectrometry. [22][23][24] However, the main issues with using such approaches, are the building up of detectors, time-consuming extraction, usage of costly instruments and separation practices.…”

mentioning

confidence: 99%

Electrochemical Sensing of Phenylbutazone using Multi-Walled Carbon Nanotube Paste Electrode in Pharmaceutical and Biological Fluids

Patil,

Megalamani,

Abbar

et al. 2024

ECS Adv.

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The electrochemical performance of phenylbutazone (PBZ) was studied using a multi-walled carbon-nanotube-modified paste electrode (MWCNT/CPE) using a variety of voltammetric tools like cyclic voltammetry (CV), linear sweep voltammetry (LSV), and square wave voltammetry (SWV). The results showed that the MWCNT/CPE exhibited remarkable electro-catalytic action towards the electrochemical oxidation of PBZ in a phosphate buffer solution of physiological pH 7 compared to a bare carbon paste electrode. The electro-kinetic parameters like heterogeneous rate constant, transfer coefficient, scan rate, pH, and involvement of electrons in electro-oxidation of PBZ was investigated. For bare CPE, the peak current was noted to be 19.53 µA with peak potential of 0.6871 V. For MWCNT/CPE, the peak current was 30.53 µA with peak potential of 0.6792 V. The anodic peak was analyzed, and the process was diffusion controlled. For the estimation of PBZ, a SWV technique was developed with great precision and accuracy, with a detection limit of 5.2 nM and a limit of quantification of 17nM, in the concentration range 1 x 10-7 to 10 x 10-6 M. The MWCNT/CPE has been used successfully for PBZ detection in injection, blood, and urine samples, with recovery rates of 98.9% to 101.5%, 96.3% to101.7% and 98.3% to 102.8%, respectively

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Direct injection HPLC method for the determination of phenylbutazone and oxyphenylbutazone in serum using a semipermeable surface column

Cited by 11 publications

References 16 publications

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

Production of antisera to phenylbutazone and oxyphenylbutazone for use in immunochemical detection assays

Electrochemical Sensing of Phenylbutazone using Multi-Walled Carbon Nanotube Paste Electrode in Pharmaceutical and Biological Fluids

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