Direct infusion electrospray ionization–ion mobility–mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling

Leng, Jiapeng; Guan, Qing; Wang, Haoyang; Cui, Jianlan; Liu, Qinghao; Zhang, Zhi‐Xu; Zhang, Manyu; Guo, Yinlong

doi:10.1016/j.aca.2015.06.029

Cited by 22 publications

(11 citation statements)

References 35 publications

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“… 22 , 30 Besides LC, ion mobility spectrometry can be coupled with MS, and fatty acid isomers may be isolated in real time during imaging. 55 , 56 …”

Section: Results and Discussionmentioning

confidence: 99%

On-Tissue Derivatization via Electrospray Deposition for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Endogenous Fatty Acids in Rat Brain Tissues

Comi

et al. 2016

Anal. Chem.

154

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Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is used for the multiplex detection and characterization of diverse analytes over a wide mass range directly from tissues. However, analyte coverage with MALDI MSI is typically limited to the more abundant compounds, which have m/z values that are distinct from MALDI matrix-related ions. On-tissue analyte derivatization addresses these issues by selectively tagging functional groups specific to a class of analytes, while simultaneously changing their molecular masses and improving their desorption and ionization efficiency. We evaluated electrospray deposition of liquid-phase derivatization agents as a means of on-tissue analyte derivatization using 2-picolylamine; we were able to detect a range of endogenous fatty acids with MALDI MSI. When compared with airbrush application, electrospray led to a 3-fold improvement in detection limits and decreased analyte delocalization. Six fatty acids were detected and visualized from rat cerebrum tissue using a MALDI MSI instrument operating in positive mode. MALDI MSI of the hippocampal area allowed targeted fatty acid analysis of the dentate gyrus granule cell layer and the CA1 pyramidal layer with a 20-μm pixel width, without degrading the localization of other lipids during liquid-phase analyte derivatization.

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“… 22 , 30 Besides LC, ion mobility spectrometry can be coupled with MS, and fatty acid isomers may be isolated in real time during imaging. 55 , 56 …”

Section: Results and Discussionmentioning

confidence: 99%

On-Tissue Derivatization via Electrospray Deposition for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Endogenous Fatty Acids in Rat Brain Tissues

Comi

et al. 2016

Anal. Chem.

154

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“…23,24 When using IM-MS, isomer species of intermediates with the same m/z can be resolved quickly and efficiently due to different drift time ( Figure 1). [25][26][27][28][29][30][31][32][33][34] Furthermore, the collision cross-section (CCS) value closely related to the size of ions could be measured based on drift time. By comparing with the theoretical CCS value, the structure identification would be more accurate.…”

Section: Introductionmentioning

confidence: 99%

Study of short-lived and early reaction intermediates in organocatalytic asymmetric amination reactions by ion-mobility mass spectrometry

Zhang

Wang

Zhang

et al. 2016

Catal. Sci. Technol.

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Reactive solvent-assisted eletrosrpay ionization coupled to ion-mobility mass spectrometry (reactive SAESI-IM-MS) was used to study the reactive intermediates in the organocatalytic asymmetric amination reaction. The transient intermediates including two isomers of carbinolamine and iminium species in the early stage of the enamine formation and previously unrecognized triazane species appearing before iminium intermediates 6 formation were successfully intercepted and characterized by reactive-SAESI-IM-MS. Moreover, the ratio of Z-and E-enamine intermediates in situ were obtained rapidly and accurately by IM-MS. Thus, a more clear and detailed landscape for understanding the mechanisms of organocatalytic asymmetric amination reactions and the origin of enantioselectivity was given, which demonstrates the remarkablely increased ability of reactive-SAESI-IM-MS in detecting and characterizating the reaction intermediates.Study of the reactive intermediates in organocatalytic asymmetric amination reactions by reactive-SAESI coupled to ion-mobility mass spectrometry

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“…Thus, without any chromatographic separation, direct fatty acids profiling in complex samples could be performed due to the great signal enhancement induced by DMPP in addition to the extra resolution that ESI–IM–MS offered. With this approach, increased levels of 16 fatty acids were detected in carcinoma tissue compared with healthy one (Leng et al, ). 2 H 0 / 2 H 6 ‐DMPP pair derivatization, combined with frozen pre‐treatment, was also utilized for the analysis of fatty acids in infant milk powders.…”

Section: Isotope‐coded Derivatization Reagents For Lc–ms Analysismentioning

confidence: 99%

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

El-Maghrabey

Kishikawa

Kuroda

2020

Biomedical Chromatography

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Currently, LC–MS has various applications in different areas such as metabolomics, pharmacokinetics, and pathological studies. Yet, matrix effects resulting from co‐existing constituents remain a major problem for LC–MS [or LC–tandem mass spectrometry (LC–MS/MS)]. Moreover, technical problems and instrumental drifts may lead to ion abundance variance. Thus, an internal standard (IS) is required to guarantee the accuracy and precision of the method. Because of their limited number, isotope‐coded derivatization (ICD) has been recently introduced to overcome this problem. For ICD, a stable heavy isotope‐coded moiety is used for labeling the standard or the control sample and the formed products can act as ISs. A light form of the reagent is used for labeling the sample. Then, both are mixed and analyzed by LC–MS(/MS). This strategy permits the identification of different unknown analytes including potential metabolites and disease biomarkers. All these attributes lead to persistent growth in the applications of ICD LC–MS(/MS) in various biomedical branches. In this article we review the ICD methods published in the last eight years for biomedical applications as well as briefly summarize other applications for environmental and food analyses as some of their used ICD reagents were further applied for analyzing biological specimens or have the potential for that.

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Direct infusion electrospray ionization–ion mobility–mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling

Cited by 22 publications

References 35 publications

On-Tissue Derivatization via Electrospray Deposition for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Endogenous Fatty Acids in Rat Brain Tissues

On-Tissue Derivatization via Electrospray Deposition for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Endogenous Fatty Acids in Rat Brain Tissues

Study of short-lived and early reaction intermediates in organocatalytic asymmetric amination reactions by ion-mobility mass spectrometry

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

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