Direct in Vivo Electrochemical Detection of Resting Dopamine Using Poly(3,4-ethylenedioxythiophene)/Carbon Nanotube Functionalized Microelectrodes

Taylor, Ian Mitchell; Patel, Nikita; Freedman, Noah C; Castagnola, Elisa; Cui, Xiufang

doi:10.1021/acs.analchem.9b02904

Cited by 70 publications

(79 citation statements)

References 87 publications

(173 reference statements)

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“…HPLC confirmed a 99% loss of DA tissue content in the denervated dSTR (in μg/g of tissue: non-lesioned, 6.3 ± 0.8; 6-OHDA, 0.03 ± 0.002). These low nanomolar levels of resting DA are consistent with studies using no net flux microdialysis [6,[47][48][49][50] and the recent square wave voltammetry study by Taylor et al [51].…”

Section: In Vitro Characterization Of Meas For Measuring Dasupporting

confidence: 89%

“…This is similar to self-referenced in vivo glutamate measures where noise that is present on both sites is removed [55,56]. Our estimated LOD for the S2 and DSRP8 MEAs, employed using the self-referencing subtraction approach, ranged from 0.3 to 1 nanomolar, which rivals the recent report of DA detection by Taylor et al using square-wave voltammetry [51]. This is seen from the recordings of d-amphetamine-induced DA transients seen in Figure 5, which required no filtering or signal averaging.…”

Section: Discussionsupporting

confidence: 78%

“…Note the high signal-to-noise of these signals that are not filtered or averaged. Typical detection limits of these signals, based on a signal-to-noise of 3, typically ranged from 0.3 to 1 nanomolar, rivaling the recent report of Taylor et al, 2019[51].…”

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confidence: 69%

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Tonic and Phasic Amperometric Monitoring of Dopamine Using Microelectrode Arrays in Rat Striatum

et al. 2020

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Here we report a novel microelectrode array recording approach to measure tonic (resting) and phasic release of dopamine (DA) in DA-rich areas such as the rat striatum and nucleus accumbens. The resulting method is tested in intact central nervous system (CNS) and in animals with extensive loss of the DA pathway using the neurotoxin, 6-hydroxyDA (6-OHDA). The self-referencing amperometric recording method employs Nafion-coated with and without m-phenylenediamine recording sites that through real-time subtraction allow for simultaneous measures of tonic DA levels and transient changes due to depolarization and amphetamine-induced release. The recording method achieves low-level measures of both tonic and phasic DA with decreased recording drift allowing for enhanced sensitivity normally not achieved with electrochemical sensors in vivo.

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Section: In Vitro Characterization Of Meas For Measuring Dasupporting

confidence: 89%

Section: Discussionsupporting

confidence: 78%

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Tonic and Phasic Amperometric Monitoring of Dopamine Using Microelectrode Arrays in Rat Striatum

et al. 2020

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“…Carbon fiber microelectrode fabrication. CFEs were fabricated as previously described in (Castagnola et al 2020;Taylor et al 2019). Briefly, borosilicate capillaries (0.4 mm ID, 0.6 mm OD; A-M systems Inc., Sequim, WA, USA), each containing a single carbon fiber (7μm diameter, T650; Cytec Carbon Fibers LLC., Piedmont, SC, USA), were pulled to a fine tip using a vertical puller (Narishige, Los Angeles, CA, USA).…”

Section: 2mentioning

confidence: 99%

3D Fuzzy Graphene Microelectrode Array for Neurotransmitter Sensing at Sub-cellular Spatial Resolution

Castagnola¹,

Garg²,

Rastogi³

et al. 2020

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<div>Dopamine (DA) is a monoamine neurotransmitter involved in the modulation of various physiological brain functions, including learning, motivation, reward, and motor functions. The development of a high sensitivity real-time sensor for multi-site detection of DA with high spatial resolution has critical implications for both neuroscience and clinical communities to improve understanding and treatments of neurological and neuropsychiatric disorders. Here, we present high-surface area out-of-plane grown three-dimensional (3D) fuzzy graphene (3DFG) microelectrode arrays (MEAs) for highly selective, sensitive, and stable DA electrochemical sensing. 3DFG microelectrodes present a remarkable sensitivity to DA (2.87 ± 0.25 nA/nM, with</div><div>LOD of 990±15 pM), the highest reported for nanocarbon MEAs using Fast Scan Cyclic Voltammetry (FSCV). The high surface area of 3DFG allows for miniaturization of electrode down to 2 x 2 μm^2, without compromising the electrochemical performance. Moreover, 3DFG MEAs are electrochemically stable under 7.2 million scans of continuous FSCV cycling, present exceptional selectivity over the most common interferents in vitro with minimum fouling by electrochemical byproducts, and can discriminate DA and serotonin (5-HT) in response to the injection of their 50:50 mixture. These results highlight the potential of 3DFG MEAs as a promising platform for FSCV based multi-site detection of DA with high sensitivity, selectivity, and spatial resolution.</div>

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“…Practical applications of such biosensors are limited by their short lifetime and poor reproducibility [ 15 , 16 , 17 ]. Alternatively, chemical sensors modified with a wide range of materials such as carbon-based materials [ 18 , 19 , 20 ], ionic liquid composites [ 21 , 22 ], metal/semiconductors [ 23 , 24 , 25 ], and organometallic polymers [ 26 , 27 ] are able to provide similar detection limits in comparison with biosensors, and enable the detection of DA in the presence of interfering compounds mimicking biological systems.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of MOF525/PEDOT Composites as Microelectrodes for Electrochemical Sensing of Dopamine

et al. 2020

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Dopamine (DA) is an important neurotransmitter responsible for the functions and activities of multiple systems in human. Electrochemical detection of DA has the advantages of fast analysis and cost-effectiveness, while a regular electrode probe is restricted to laboratory use because the probe size is too large to be suitable for an in vivo or in vitro analysis. In this study, we have developed porphyrin-based metal organic framework (MOF525) and poly(3,4-ethylenedioxythiophene) (PEDOT)-based composites to modify microelectrode for DA detection. Two types of PEDOT monomers with different functional groups were investigated in this study. By varying the monomer ratios, electrolyte concentrations, and electropolymerization temperature, it was found that the PEDOT monomer containing carboxylic group facilitated the formation of regular morphology during the electropolymerization process. The uniform morphology of the PEDOT promoted the electron transmission efficiency in the same direction, while the MOF525 provided a large reactive surface area for electrocatalysis of DA. Thus, the MOF525/PEDOT composite improved the sensitivity-to-noise ratio of DA signaling, where the sensitivity reached 11 nA/μM in a good linear range of 4–100 µM. In addition, porphyrin-based MOF could also increase the selectivity to DA against other common clinical interferences, such as ascorbic acid and uric acid. The as-synthesized microelectrode modified with MOF525/PEDOT in this study exhibited great potential in real time analysis.

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Direct in Vivo Electrochemical Detection of Resting Dopamine Using Poly(3,4-ethylenedioxythiophene)/Carbon Nanotube Functionalized Microelectrodes

Cited by 70 publications

References 87 publications

Tonic and Phasic Amperometric Monitoring of Dopamine Using Microelectrode Arrays in Rat Striatum

Tonic and Phasic Amperometric Monitoring of Dopamine Using Microelectrode Arrays in Rat Striatum

3D Fuzzy Graphene Microelectrode Array for Neurotransmitter Sensing at Sub-cellular Spatial Resolution

Synthesis of MOF525/PEDOT Composites as Microelectrodes for Electrochemical Sensing of Dopamine

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