Direct in vitro effect of LH and steroids on leptin gene expression and leptin secretion by porcine luteal cells during the mid-luteal phase of the estrous cycle

Siawrys, Gabriela; Smolińska, Nina

doi:10.1016/j.repbio.2012.10.003

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Approximately 1 μg of RNA was reverse-transcribed into cDNA in a total volume of 20 μl with 0.5 μg oligo (dt) 15 primer using the Omniscript RT Kit (Qiagen, USA) at 37 o C for one hour. A qRT-PCR analysis was performed according to method described previously (Siawrys and Smolinska, 2012) with the use of ABI Prism 7000 Sequence Detection System (Applied Biosystems, Foster City, CA). cDNA samples were amplified in a final reaction volume of 25 μl containing 1x SYBR Green PCR-Master Mix (Applied Biosystems, Foster City, CA, USA) and the respective primers (400 nmol/l) using the following cycling program: 2 min at 50 o C, 10 min at 95 o C, followed by 15 sec at 95 o C and 1 min at 59 o C for a total of 46 cycles.…”

Section: Total Rna Isolation Cdna Synthesis and Real-time Pcr Analysismentioning

confidence: 99%

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

Siawrys¹,

Gajewska²

2017

Polish Journal of Veterinary Sciences

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Leptin modulates reproductive activity but its potential influence on LH secretion from anterior pituitary (AP) cells during implantation period in pigs (days 14-16 of pregnancy) remained unexplored. This study focused on determination whether leptin affects basal and GnRH-induced LH secretion and intracellular accumulation and whether leptin receptor (OB-Rb) mRNA is expressed in the AP gland during implantation in pigs. Four individual AP glands were developed into separate primary cultures. 2x10 5 cells/ml were preincubated (72 h) and next, for 3.5 h, experimentally treated with GnRH (100 ng/ml), leptin (10 -11 , 10 -9, 10 -7, 10 -6 M) alone, or given in respective combinations with GnRH. In the AP gland, OB-Rb mRNA expression was determined by real-time PCR method. Leptin activated LH secretion and its concentration-dependent effect was observed as stimulation shown in a full range tested (culture 1) and exhibited only at 10 -6 M (culture 2). A pooled data analysis revealed that basal LH secretion increased at 10 -9 , 10 -7 and 10 -6 M, but GnRH-induced LH release decreased at 10 -6 M. Leptin down-regulated GnRH-induced LH secretion in all cultures, but only culture 3 exhibited sensitivity for all concentrations tested. Basal LH accumulation was activated in culture 1 (at 10 -11 M) and inhibited in culture 4 (at 10 -9 M). In the presence of GnRH leptin up-regulated LH accumulation with individual culture leptin-sensitivity (culture 1-3), while down-regulated LH accumulation in culture 4. Obtained data indicate that OB-Rb mRNA is expressed in the AP gland and leptin alone and in combination with GnRH specifically modulates LH activity during early pregnancy in pigs.

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Section: Total Rna Isolation Cdna Synthesis and Real-time Pcr Analysismentioning

confidence: 99%

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

Siawrys¹,

Gajewska²

2017

Polish Journal of Veterinary Sciences

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“…These results suggest a lack of leptin effect on ovarian functions at this stage of the cycle in this species, and that the effect of leptin on steroidogenesis may be species-specific. Furthermore, E 2 and P 4 increased leptin gene expression and leptin secretion by the porcine LCs, indicating that steroid hormones affect leptin mRNA levels and leptin secretion during the mid-luteal phase of the estrous cycle and early pregnancy [313,314]. Thus, it seems that not only does leptin affect steroid hormones secretion, but steroids can also affect leptin expression.…”

Section: Leptinmentioning

confidence: 95%

“…The differentiated expression of the leptin system components dependent on the stage of CL functioning suggests that it may be affected by steroid hormones [292,293,296,297,299,300,306,308]. The in vitro studies of isolated porcine LCs revealed an up-regulatory role of E 2 , P 4 , and LH on leptin transcript content and the protein secretion [313,314]. Moreover, the study of Ryan et al showed that human chorionic gonadotropin (hCG) may also increase the mRNA level of the adipokine and both variants of the leptin receptor, LEPRa, and LEPRb, in the murine CL [309].…”

Section: Leptinmentioning

confidence: 98%

New Aspects of Corpus Luteum Regulation in Physiological and Pathological Conditions: Involvement of Adipokines and Neuropeptides

Mlyczyńska

Kieżun

Kurowska

et al. 2022

Cells

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The corpus luteum is a small gland of great importance because its proper functioning determines not only the appropriate course of the estrous/menstrual cycle and embryo implantation, but also the subsequent maintenance of pregnancy. Among the well-known regulators of luteal tissue functions, increasing attention is focused on the role of neuropeptides and adipose tissue hormones—adipokines. Growing evidence points to the expression of these factors in the corpus luteum of women and different animal species, and their involvement in corpus luteum formation, endocrine function, angiogenesis, cells proliferation, apoptosis, and finally, regression. In the present review, we summarize the current knowledge about the expression and role of adipokines, such as adiponectin, leptin, apelin, vaspin, visfatin, chemerin, and neuropeptides like ghrelin, orexins, kisspeptin, and phoenixin in the physiological regulation of the corpus luteum function, as well as their potential involvement in pathologies affecting the luteal cells that disrupt the estrous cycle.

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“…The reproductive physiology of sows is affected by various factors including the environment [3,7], hormones [10,15,16], diet [4,9], and genetics [5,13]. These factors are important not only for increasing litter sizes on commercial farms but also improving embryo production by somatic cell nuclear transfer (SCNT) in the laboratory.…”

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confidence: 99%

Relationship between pregnancy rate and serum progesterone concentration in cases of porcine embryo transfer

et al. 2014

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The level of P4 at the time of embryo transfer (ET) is important. P4 concentrations and numbers of corpora lutea for 126 recipients were evaluated. Nuclear transfer embryos were transferred into 126 surrogates. 11 maintained their pregnancy until full-term delivery, 17 miscarried, and implantation failed in 98 animals. P4 levels in the full-term group were significantly different from those of the pigs that aborted or in which implantation failed (p < 0.05). However, the numbers of corpora lutea were not significantly different. These findings indicate that the concentration of progesterone can be an important factor for successful ET in pigs.

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Direct in vitro effect of LH and steroids on leptin gene expression and leptin secretion by porcine luteal cells during the mid-luteal phase of the estrous cycle

Cited by 5 publications

References 38 publications

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig

New Aspects of Corpus Luteum Regulation in Physiological and Pathological Conditions: Involvement of Adipokines and Neuropeptides

Relationship between pregnancy rate and serum progesterone concentration in cases of porcine embryo transfer

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