Direct Imaging of Carboxymethyl Cellulose-mediated Aggregation of Lactic Acid Bacteria Using Dark-field Microscopy

Nakao, Hidenobu; Saito, Katsuichi; Tomita, Satoru; Magariyama, Yukio; Kaizuka, Yoshihisa; Takeda, Yoshihiko

doi:10.2116/analsci.32.1047

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Dark-field microscopy is an interesting optical technique that has been successfully used to image bacteria [ 40 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ] and protozoa [ 102 , 115 ] due to its very low background, simple construction, portability, and low cost. Since plasma nanoparticles exhibit strong scattering to visible light, dark-field microscopy is a powerful tool for imaging and localization of noble metal nanoparticles in single cell analysis [ 101 , 109 , 116 , 117 ].…”

Section: Label-free Detection Of Bacteria By Dark-field Microscopymentioning

confidence: 99%

Optical Methods for Label-Free Detection of Bacteria

et al. 2022

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Pathogenic bacteria are the leading causes of food-borne and water-borne infections, and one of the most serious public threats. Traditional bacterial detection techniques, including plate culture, polymerase chain reaction, and enzyme-linked immunosorbent assay are time-consuming, while hindering precise therapy initiation. Thus, rapid detection of bacteria is of vital clinical importance in reducing the misuse of antibiotics. Among the most recently developed methods, the label-free optical approach is one of the most promising methods that is able to address this challenge due to its rapidity, simplicity, and relatively low-cost. This paper reviews optical methods such as surface-enhanced Raman scattering spectroscopy, surface plasmon resonance, and dark-field microscopic imaging techniques for the rapid detection of pathogenic bacteria in a label-free manner. The advantages and disadvantages of these label-free technologies for bacterial detection are summarized in order to promote their application for rapid bacterial detection in source-limited environments and for drug resistance assessments.

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Section: Label-free Detection Of Bacteria By Dark-field Microscopymentioning

confidence: 99%

Optical Methods for Label-Free Detection of Bacteria

et al. 2022

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“…Thus, LAB recovered with this method could be clearly observed by optical microscopy and SEM, meaning that the total cell counts, morphological changes and cell viability could be directly detected at the single-cell level. [9][10][11]…”

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confidence: 99%

Simple and Rapid Method for Separating Lactic Acid Bacteria from Commercially Prepared Yogurt

Nakao

Kim

2019

ANAL. SCI.

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We have reported a simple method for separating lactic acid bacteria (LAB) from yogurt. This method is based on the process of destructions and denaturation of casein micelle aggregates by vortexing, and can supply samples containing only LAB. Recovered LAB were clearly observable by microscopy, meaning that morphological changes could be directly detected at the single-cell level. This method will be a helpful tool for the analyzing various LAB, including their enzyme activity and protein expression.

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Aggregation of Lactobacillus brevis associated with decrease in pH by glucose fermentation

Saito

Tomita

Nakamura

2019

Bioscience, Biotechnology, and Biochemistry

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Some Lactobacillus brevis strains were found to aggregate upon the addition of glucose, which resulted in glucose fermentation and pH decrease. Surface layer proteins (Slp) that represented the outermost layer of the bacteria decreased under these low pH conditions, probably because of the partial detachment of Slp from the cell surface triggered by the acidic environment. Similar observations of decreased Slp and aggregation were observed under the culture conditions, confirming that L. brevis aggregation was due to the partial Slp detachment under the acidic conditions of glucose fermentation. Such Slp detachment might affect the electrostatic nature of L. brevis cells by initiating the formation of irregular charge across the L. brevis cell surface, thereby leading to aggregation. These observations would be useful for elucidating the aggregation mechanism of lactic acid bacteria, which was considered to be involved in the probiotic effect of the bacteria.

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Direct Imaging of Carboxymethyl Cellulose-mediated Aggregation of Lactic Acid Bacteria Using Dark-field Microscopy

Cited by 3 publications

References 26 publications

Optical Methods for Label-Free Detection of Bacteria

Optical Methods for Label-Free Detection of Bacteria

Simple and Rapid Method for Separating Lactic Acid Bacteria from Commercially Prepared Yogurt

Aggregation of Lactobacillus brevis associated with decrease in pH by glucose fermentation

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