Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now <i>S. aureus</i> and PBP2a Tests

Heraud, Sandrine; Freydière, Anne-Marie; Doléans-Jordheim, Anne; Bes, Michèle; Tristan, Anne; Vandenesch, François; Laurent, François; Dauwalder, Olivier

doi:10.3343/alm.2015.35.4.454

Cited by 13 publications

(9 citation statements)

References 15 publications

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“…However antimicrobial susceptibility testing is also essential for selecting the optimal treatment. Rapid resistance testing methods have been introduced in clinical microbiology laboratories for the detection of third generation cephalosporin-resistant Enterobacteriaceae (EB) and Pseudomonas aeruginosa or for the detection of methicillin resistance in Staphylococcus aureus [ 10 , 11 , 12 , 13 ]. These partial susceptibility tests have been validated for their diagnostic performance although their clinical impact has not been thoroughly assessed.…”

Section: Introductionmentioning

confidence: 99%

Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

et al. 2016

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Shortening the turn-around time (TAT) of positive blood culture (BC) identification (ID) and susceptibility results is essential to optimize antimicrobial treatment in patients with sepsis. We aimed to evaluate the impact on antimicrobial prescription of a modified workflow of positive BCs providing ID and partial susceptibility results for Enterobacteriaceae (EB), Pseudomonas aeruginosa and Staphylococcus aureus on the day of BC positivity detection. This study was divided into a pre-intervention period (P0) with a standard BC workflow followed by 2 intervention periods (P1, P2) with an identical modified workflow. ID was performed with MALDI-TOF MS from blood, on early or on overnight subcultures. According to ID results, rapid phenotypic assays were realized to detect third generation cephalosporin resistant EB/P. aeruginosa or methicillin resistant S. aureus. Results were transmitted to the antimicrobial stewardship team for patient’s treatment revision. Times to ID, to susceptibility results and to optimal antimicrobial treatment (OAT) were compared across the three study periods. Overall, 134, 112 and 154 positive BC episodes in P0, P1 and P2 respectively were included in the analysis. Mean time to ID (28.3 hours in P0) was reduced by 65.3% in P1 (10.2 hours) and 61.8% in P2 (10.8 hours). Mean time to complete susceptibility results was reduced by 27.5% in P1 and 27% in P2, with results obtained after 32.4 and 32.6 hours compared to 44.7 hours in P0. Rapid tests allowed partial susceptibility results to be obtained after a mean time of 11.8 hours in P1 and 11.7 hours in P2. Mean time to OAT was decreased to 21.6 hours in P1 and to 17.9 hours in P2 compared to 36.1 hours in P0. Reducing TAT of positive BC with MALDI-TOF MS ID and rapid susceptibility testing accelerated prescription of targeted antimicrobial treatment thereby potentially improving the patients’ clinical outcome.

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Section: Introductionmentioning

confidence: 99%

Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

et al. 2016

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“…Several Staphylococcus species, such as Staphylococcus epidermis (S. epidermis), Staphylococcus haemolyticus (S. haemolyticus), and the coagulase negative species, have been isolated from ewe's milk and were found to produce one or several staphylococcus enterotoxins [2] and consequently there is a need for methods to specifically discriminate S. aureus from other staphylococci and non-staphylococci as quickly as possible. Conventional identification methods and novel assays based on immunofluorescence probe, DNA microarray, surface enhanced laser desorption and ionization time of flight mass spectrometry, and whole-cell SELEX methods are time-consuming and may yield false-positive or false-negative results, and misclassifications with automated susceptibility testing systems or commercially available latex agglutination kits have been reported recently [4][5][6][7][8][9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Staphylococcus aureus isolated from clinical samples by specific PCR assay targeting the signal transduction gene

Liu¹

2017

JPP

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Staphylococcus aureus (S. aureus) is a common, Gram-positive species that is pathogenic in both human and animals. Rapid and direct identification of this bacterium specifically from clinical specimens would be useful in improving the diagnosis of S. aureus infections in the clinical microbiology laboratory. Although, some molecular identification assays are reported, some false-positive cases are described; it is an urgent need to develop a specific diagnostic method. Currently, the sensitivity and specificity of polymerase chain reaction targeting a signal transduction gene (vick) for the diagnosis of S. aureus isolates in clinical samples was developed. The assay is sensitive enough to detect 5500 copies of a cloned fragment of the S. aureus vick gene. This PCR assay can be used for rapid clinical diagnosis of S. aureus infection and detection of the pathogen in food samples.

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“…The Verigene Test uses a microfluidic cassette to identify 37 different microorganisms employing nanoparticles 17–23 and BinaxNOW is an immunochromatographic assay for a variety of infectious agents (methicillin-resistant S. aureus , E. coli , Streptococcus pneumoniae , etc.). 24 Both of these methods are destructive, meaning the final product cannot be used for further diagnostic testing. Accelerate Diagnostics also uses positive blood culture for their diagnostic instrument.…”

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confidence: 99%

Rapid Detection of Bacteria from Blood with Surface-Enhanced Raman Spectroscopy

et al. 2016

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Traditional methods for identifying pathogens in bacteremic patients are slow (24–48+ h). This can lead to physicians making treatment decisions based on an incomplete diagnosis and potentially increasing the patient’s mortality risk. To decrease time to diagnosis, we have developed a novel technology that can recover viable bacteria directly from whole blood and identify them in less than 7 h. Our technology combines a sample preparation process with surface-enhanced Raman spectroscopy (SERS). The sample preparation process enriches viable microorganisms from 10 mL of whole blood into a 200 μL aliquot. After a short incubation period, SERS is used to identify the microorganisms. We further demonstrated that SERS can be used as a broad detection method, as it identified a model set of 17 clinical blood culture isolates and microbial reference strains with 100% identification agreement. By applying the integrated technology of sample preparation and SERS to spiked whole blood samples, we were able to correctly identify both Staphylococcus aureus and Escherichia coli 97% of the time with 97% specificity and 88% sensitivity.

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Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now S. aureus and PBP2a Tests

Cited by 13 publications

References 15 publications

Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

Molecular identification of Staphylococcus aureus isolated from clinical samples by specific PCR assay targeting the signal transduction gene

Rapid Detection of Bacteria from Blood with Surface-Enhanced Raman Spectroscopy

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