Direct hydrogen production from lignocellulose by the newly isolated Thermoanaerobacterium thermosaccharolyticum strain DD32

Sheng, Tao; Gao, Lingfang; Zhao, Lei; Liu, Wenzong; Wang, Aijie

doi:10.1039/c5ra20000h

Cited by 36 publications

(16 citation statements)

References 48 publications

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“…Thermoanaerobacterium spp. has been demonstrated to have the ability to consume complex substrates to produce hydrogen, such as cellulose [10, 11], starch [5, 19], xylan [5], glucose [8, 19, 24], xylose [8, 19, 27] and sucrose [8, 13, 28]. The corresponding hydrogen yields from different isolated T. thermosaccharolyticum stains are listed in Table 1.…”

Section: Resultsmentioning

confidence: 99%

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Direct hydrogen production from dilute-acid pretreated sugarcane bagasse hydrolysate using the newly isolated Thermoanaerobacterium thermosaccharolyticum MJ1

Zhu

2017

Microb Cell Fact

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BackgroundEnergy shortage and environmental pollution are two severe global problems, and biological hydrogen production from lignocellulose shows great potential as a promising alternative biofuel to replace the fossil fuels. Currently, most studies on hydrogen production from lignocellulose concentrate on cellulolytic microbe, pretreatment method, process optimization and development of new raw materials. Due to no effective approaches to relieve the inhibiting effect of inhibitors, the acid pretreated lignocellulose hydrolysate was directly discarded and caused environmental problems, suggesting that isolation of inhibitor-tolerant strains may facilitate the utilization of acid pretreated lignocellulose hydrolysate.ResultsThermophilic bacteria for producing hydrogen from various kinds of sugars were screened, and the new strain named MJ1 was isolated from paper sludge, with 99% identity to Thermoanaerobacterium thermosaccharolyticum by 16S rRNA gene analysis. The hydrogen yields of 11.18, 4.25 and 2.15 mol-H2/mol sugar can be reached at an initial concentration of 5 g/L cellobiose, glucose and xylose, respectively. The main metabolites were acetate and butyrate. More important, MJ1 had an excellent tolerance to inhibitors of dilute-acid (1%, g/v) pretreated sugarcane bagasse hydrolysate (DAPSBH) and could efficiently utilize DAPSBH for hydrogen production without detoxication, with a production higher than that of pure sugars. The hydrogen could be quickly produced with the maximum hydrogen production reached at 24 h. The hydrogen production reached 39.64, 105.42, 111.75 and 110.44 mM at 20, 40, 60 and 80% of DAPSBH, respectively. Supplementation of CaCO3 enhanced the hydrogen production by 21.32% versus the control.ConclusionsThese results demonstrate that MJ1 could directly utilize DAPSBH for biohydrogen production without detoxication and can serve as an excellent candidate for industrialization of hydrogen production from DAPSBH. The results also suggest that isolating unique strains from a particular environment offers an ideal way to conquer the related problems.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0692-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…Generally, substrate concentration largely affects the hydrogen production [10, 27]. The initial xylose concentration affected the production of hydrogen and metabolites (Fig.…”

Section: Resultsmentioning

confidence: 99%

Direct hydrogen production from dilute-acid pretreated sugarcane bagasse hydrolysate using the newly isolated Thermoanaerobacterium thermosaccharolyticum MJ1

Zhu

2017

Microb Cell Fact

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“…Hence, several pre-treatments have been streamlined to ensure that lignocellulosic biomass can be broken down into oligosaccharides that can be more readily utilized by hydrogenproducing microbes. 15 However, current pre-treatment methods based on acid, alkali or cellulase have conspicuous disadvantages, such as high consumption, strict conditions, timeconsuming processes and formative fermentation inhibitors. 16 A highly integrated one-step process named consolidated bioprocess (CBP) has been investigated for several years.…”

Section: Introductionmentioning

confidence: 99%

“…To date, a few microorganisms capable of producing hydrogen, which prefer oligosaccharides to cellulose, have been identied. 15,[19][20][21] Therefore, to make lignocellulosic hydrogen production competitive, more microorganisms that can produce lignocellulosic hydrogen without pre-treatment should be developed.…”

Section: Introductionmentioning

confidence: 99%

Lignocellulosic hydrogen production using dark fermentation byClostridium lentocellumstrain Cel10 newly isolated fromAiluropoda melanoleucaexcrement

Lu-yan

Liu

et al. 2019

RSC Adv.

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“…To verify the pretreatment efficiency, rice straw was saccharied by R. thermocellum M3 isolated by Sheng et al 22 The saccharication process was carried out at 60 C for 72 h using MA medium with 5 g L À1 pretreated/untreated rice straw as carbon source, as described by Sheng et al 23 Samples were taken every 6 h to determine oligosaccharide concentration, cell biomass, substrate degradation, and liquid-metabolites.…”

Section: Saccharication Of Pretreated and Untreated Rice Strawmentioning

confidence: 99%

Fenton pre-treatment of rice straw with citric acid as an iron chelate reagent for enhancing saccharification

et al. 2017

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The Fenton reagent has recently been verified as being effective for lignocellulose pretreatment. However, the Fenton reaction can only work under acidic conditions, leading to severe environmental pollution and increasing the cost for sewage treatment. This has made identification of iron chelates that could enhance Fenton degradation under neutral conditions important. In this study, citric acid, a non-toxic polyhydroxy carboxylic acid, was introduced as an iron chelate in Fenton pre-treatment of rice straw. Results demonstrated that citric acid has the ability to (i) maintain the oxidation capacity of the Fenton reagent under near-neutral conditions, (ii) enhance the degradation of lignin and reduce the crystallinity of rice straw from 0.41 to 0.21, (iii) promote the saccharification of rice straw (the maximum saccharification ratio of 35.7% was two-fold higher than that for the untreated rice straw), and (iv) elevate the maximum activity of b-glucosidase from 0.33 IU mL À1 to 0.61 IU mL À1 . Moreover, the effect of operational parameters such as pH and processing time on the saccharification of rice straw by Fenton pretreatment with and without citric acid has been demonstrated. The findings strongly support Fenton plus citric acid as an efficient and environmentally friendly lignocellulose pretreatment method.

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Direct hydrogen production from lignocellulose by the newly isolated Thermoanaerobacterium thermosaccharolyticum strain DD32

Abstract: The comparison of hydrogen production by conventional process and consolidated bioprocessing.

Cited by 36 publications

References 48 publications

Direct hydrogen production from dilute-acid pretreated sugarcane bagasse hydrolysate using the newly isolated Thermoanaerobacterium thermosaccharolyticum MJ1

Direct hydrogen production from dilute-acid pretreated sugarcane bagasse hydrolysate using the newly isolated Thermoanaerobacterium thermosaccharolyticum MJ1

Lignocellulosic hydrogen production using dark fermentation byClostridium lentocellumstrain Cel10 newly isolated fromAiluropoda melanoleucaexcrement

Fenton pre-treatment of rice straw with citric acid as an iron chelate reagent for enhancing saccharification

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