Direct high‐resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Bereman, Michael S.; Nyadong, Leonard; Fernández, Facundo M.; Muddiman, David C.

doi:10.1002/rcm.2759

Cited by 83 publications

(65 citation statements)

References 18 publications

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“…The 2þ charge state of bradykinin, desorbed from Teflon and nitrile polymer surfaces, was also observed in the DESI-orbitrap experiment (Hu et al, 2006b). DESI has also recently been coupled to an FT-ICR instrument for the direct analysis of peptide and proteins (Bereman et al, 2006).…”

Section: Ambient Ionizationmentioning

confidence: 84%

Orbitrap mass spectrometry: Instrumentation, ion motion and applications

Perry

Cooks

Noll

2008

Mass Spectrometry Reviews

402

290

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Since its introduction, the orbitrap has proven to be a robust mass analyzer that can routinely deliver high resolving power and mass accuracy. Unlike conventional ion traps such as the Paul and Penning traps, the orbitrap uses only electrostatic fields to confine and to analyze injected ion populations. In addition, its relatively low cost, simple design and high space‐charge capacity make it suitable for tackling complex scientific problems in which high performance is required. This review begins with a brief account of the set of inventions that led to the orbitrap, followed by a qualitative description of ion capture, ion motion in the trap and modes of detection. Various orbitrap instruments, including the commercially available linear ion trap–orbitrap hybrid mass spectrometers, are also discussed with emphasis on the different methods used to inject ions into the trap. Figures of merit such as resolving power, mass accuracy, dynamic range and sensitivity of each type of instrument are compared. In addition, experimental techniques that allow mass‐selective manipulation of the motion of confined ions and their potential application in tandem mass spectrometry in the orbitrap are described. Finally, some specific applications are reviewed to illustrate the performance and versatility of the orbitrap mass spectrometers. © 2008 Wiley Periodicals, Inc., Mass Spec Rev 27: 661–699, 2008

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Section: Ambient Ionizationmentioning

confidence: 84%

Orbitrap mass spectrometry: Instrumentation, ion motion and applications

Perry

Cooks

Noll

2008

Mass Spectrometry Reviews

402

290

View full text Add to dashboard Cite

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“…Analytes present at the surface are desorbed and ionized by the incoming plume and subsequently transferred to the mass spectrometer inlet by the influence of the applied potential and the pressure differential between atmospheric pressure and the low-pressure region of the mass analyzer. To date, DESI has found many applications including forensic analysis [2][3][4][5], explosives detection [6 -9], chemical warfare agent detection [3, 10 -12], imaging [13][14][15], pharmaceutical analysis [16 -24], natural product characterization [2,25], polymer analysis [26,27], metabolomics [2, 28 -31], proteomics [2,[32][33], and glycomics [34].…”

Section: Esorption Electrospray Ionization (Desi)mentioning

confidence: 99%

The influence of material and mesh characteristics on transmission mode desorption electrospray ionization

Chipuk

Brodbelt

2008

J. Am. Soc. Mass Spectrom.

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Adaptation of desorption electrospray ionization to a transmission mode (TM-DESI) entails passing an electrospray plume through a sample that has been deposited onto a mesh substrate. A combination of mass spectrometry and fluorescence microscopy studies is used to illustrate the critical role material composition, mesh open space, and mesh fiber diameter play on the transmission, desorption, and ionization process. Substrates with open spaces less than 150 m and accompanying minimal strand diameters produce less scattering of the plume and therefore favor transmission. Larger strand diameters typically encompass larger open spaces, but the increase in the surface area of the strand increases plume scattering as well as solvent and analyte spreading on the mesh. Polypropylene (PP), ethylene tetrafluoroethylene (ETFE), and polyetheretherketone (PEEK) materials afford much better desorption than similarly sized polyethylene terephthalate (PETE) or nylon-6,6 (PA66) substrates. Ultimately, the manner in which the electrospray plume interacts with the mesh as it is transmitted through the substrate is shown to be critical to performing and optimizing TM-DESI analyses. In addition, evidence is presented for analyte dependent variations in the desorption mechanisms of dry and solvated samples. . In DESI, ions are produced by directing charged solvent droplets from an electrospray source toward a sample that is either a bulk material in its native state (e.g., pharmaceutical tablet) or one that has been deposited from solution onto a sampling surface. Analytes present at the surface are desorbed and ionized by the incoming plume and subsequently transferred to the mass spectrometer inlet by the influence of the applied potential and the pressure differential between atmospheric pressure and the low-pressure region of the mass analyzer. To date, DESI has found many applications including forensic analysis [2][3][4][5] Recent adaptations of DESI, including geometry independent DESI in gas tight enclosures [35] and transmission mode desorption electrospray ionization (TM-DESI), have been developed to reduce the geometry dependence of DESI experiments [36]. In the transmission DESI mode the sample is not deposited onto a continuous solid surface but rather onto a sampling mesh. In this adaptation, the incident spray angle and collection angle are fixed at 0°and the spray is transmitted through the sample (Figure 1). Along with the simplification of the experimental geometry, the transmission mode also allows convenient analysis of both dry (i.e., following evaporation of the deposition solvent) and wet (i.e., solvated) samples with similar performance characteristics to those achieved using traditional DESI [36].Surface variables including the chemical composition, porosity, texture, and electrical conductivity of the substrate have been reported to affect DESI analyses [2,24,[37][38][39]. Dramatic reductions in response have been noted for high conductivity surfaces due to neutralization of the incoming ion plume at the su...

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“…Although GC-MS instruments currently dominate the portable mass spectrometry industry, estimates indicate that the confidence for molecule identification by MS/MS is about that provided with GC-MS (when combining MS information with retention time) [15]. The MS/MS-capable CITbased instrument enables chromatography-free analysis for high confidence in situ molecule identification with 100-fold faster analysis times than standard GC-MS analyses.Atmospheric pressure desorption ionization techniques, including DESI and DART, are capable of generating ions of diverse chemical nature directly from solid surfaces for MS/MS analysis [10,[16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. In forensic [26,31], security [19,32], and quality control applications [17,27], desorption techniques provide actionable chemical information through rapid, definitive analyses.…”

mentioning

confidence: 99%

Implementation of DART and DESI ionization on a fieldable mass spectrometer

Wells¹,

Roth²,

Keil³

et al. 2008

J. Am. Soc. Mass Spectrom.

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A recently developed prototype mobile laboratory mass spectrometer, incorporating an atmospheric pressure ionization (API) interface, is described. This system takes advantage of the small size, lower voltage requirements, and tandem MS abilities of the cylindrical ion trap mass analyzer. The prototype API MS uses small, low-power pumps to fit into a 0.1-m 3 self-contained package weighing <45 kg. This instrument has been adapted to allow rapid interfacing to electrospray ionization, desorption electrospray ionization, and direct analysis in real-time sources. Initial data indicate that these techniques provide rapid detection and identification of compounds for quality control, homeland security, and forensic applications. In addition, this instrument is self-contained and compact, making it ideally extensible to mobile laboratory and field analyses. Initial MS and MS/MS data for analyses of drugs, food, and explosives are presented herein. O ver the past 15 years, efforts toward implementing mass spectrometry (MS) in the field have steadily grown with particular focus on environmental, forensic, defense, and security applications [I, 2]. Many of the previously developed fieldable MS instruments use gas chromatography (GC-MS) because of the high degree of confidence obtained using GC retention times and electron ionization (EI) mass spectral matching [3][4][5][6]. The specificity of GC-MS comes at the expense of time, with individual analyses often taking in excess of 10 min (not including sample preparation). Reducing the amount of time required for chemical identification requires separation-free MS. In the absence of chromatography, tandem MS (MS/MS) improves confidence for the identification of individual components in mixtures [7]. With spectral acquisition rates > 1 Hz, atmospheric pressure ionization (API) techniques such as electrospray ionization (ESI), desorption electro spray (DESI), and direct analysis in real time (DART), a fieldable API MS system capable of MS/MS is of high value to environmental, forensic, defense, and force protection agencies. There have been few attempts to construct fieldable MS instruments with an API interface to date [8][9][10][11] because of the difficulty of displacing the gas load of the atmospheric inlet.The cylindrical ion trap (CIT)-a simplified geometry of the hyperbolic quadrupole ion trap capable of Address reprint requests to Dr. Mitch Wells, Griffin Analytical Technologies, LLC, R&D, 3000 Kent Avenue, West Lafayette, IN 47906, USA. E-mail: mitch.wells@icxt.com performing MS n analyses-has been shown to be amenable to miniaturization [12][13][14] while maintaining benchtop-quality performance characteristics. The miniaturized CIT enables a smaller vacuum system with smaller pumps that consume less power, providing a fieldable instrument. Although GC-MS instruments currently dominate the portable mass spectrometry industry, estimates indicate that the confidence for molecule identification by MS/MS is about that provided with GC-MS (when combining MS information w...

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Direct high‐resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Cited by 83 publications

References 18 publications

Orbitrap mass spectrometry: Instrumentation, ion motion and applications

Orbitrap mass spectrometry: Instrumentation, ion motion and applications

The influence of material and mesh characteristics on transmission mode desorption electrospray ionization

Implementation of DART and DESI ionization on a fieldable mass spectrometer

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