Direct genome sequencing of respiratory viruses from low viral load clinical specimens using target capture sequencing technology
Nobuhiro Takemae,
Yumani Kuba,
Kunihiko Oba
et al.
Abstract:The use of metagenomic next-generation sequencing technology to obtain complete viral genome sequences directly from clinical samples with low viral load remains challenging—especially in the case of respiratory viruses—due to the low copy number of viral versus host genomes. To overcome this limitation, target capture sequencing for the enrichment of specific genomes has been developed and applied for direct genome sequencing of viruses. However, as the efficiency of enrichment varies depending on the probes,… Show more
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