Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun

Kuo, Chia-Feng; Wang, Jeng Hwan; Yang, Ning‐Sun

doi:10.1385/1-59259-141-8:137

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…We also reported that such ballistic bombardment can systematically generate evenly distributed, microscopic tissue tracks, creating mild, defined tissue and microvascular wounding in target skin areas [59,60]. We propose that anti-cancer vaccines may be optimized by making use of the particle bombardment technology as a systematic, highly effective and multiple site-delivery mode for the pretreatment of tumor-bearing mice areas (especially for the high number, metastasized, microscopic melanoma nodules), before administration of DC-based vaccines.…”

Section: Discussionmentioning

confidence: 99%

Specific microtubule-depolymerizing agents augment efficacy of dendritic cell-based cancer vaccines

Wen

Chen

et al. 2011

J Biomed Sci

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BackgroundDamage-associated molecular patterns (DAMPs) are associated with immunogenic cell death and have the ability to enhance maturation and antigen presentation of dendritic cells (DCs). Specific microtubule-depolymerizing agents (MDAs) such as colchicine have been shown to confer anti-cancer activity and also trigger activation of DCs.MethodsIn this study, we evaluated the ability of three MDAs (colchicine and two 2-phenyl-4-quinolone analogues) to induce immunogenic cell death in test tumor cells, activate DCs, and augment T-cell proliferation activity. These MDAs were further evaluated for use as an adjuvant in a tumor cell lysate-pulsed DC vaccine.ResultsThe three test phytochemicals considerably increased the expression of DAMPs including HSP70, HSP90 and HMGB1, but had no effect on expression of calreticulin (CRT). DC vaccines pulsed with MDA-treated tumor cell lysates had a significant effect on tumor growth, showed cytotoxic T-lymphocyte activity against tumors, and increased the survival rate of test mice. In vivo antibody depletion experiments suggested that CD8+ and NK cells, but not CD4+ cells, were the main effector cells responsible for the observed anti-tumor activity. In addition, culture of DCs with GM-CSF and IL-4 during the pulsing and stimulation period significantly increased the production of IL-12 and decreased production of IL-10. MDAs also induced phenotypic maturation of DCs and augmented CD4+ and CD8+ T-cell proliferation when co-cultured with DCs.ConclusionsSpecific MDAs including the clinical drug, colchicine, can induce immunogenic cell death in tumor cells, and DCs pulsed with MDA-treated tumor cell lysates (TCLs) can generate potent anti-tumor immunity in mice. This approach may warrant future clinical evaluation as a cancer vaccine.

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Section: Discussionmentioning

confidence: 99%

Specific microtubule-depolymerizing agents augment efficacy of dendritic cell-based cancer vaccines

Wen

Chen

et al. 2011

J Biomed Sci

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“…A helium gas-driven gene gun was used for most in vivo and all in vitro DNA transfections as previously described [30]. Plasmid DNA was precipitated onto 1 or 2 µm gold particles using the spermidine/CaCl 2 coating method.…”

Section: In Vivo or In Vitro Gene-gun-mediated Transgene Expressionmentioning

confidence: 99%

Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot‐and‐mouth disease virus

Yang

Wang

Lin

et al. 2005

The Journal of Gene Medicine

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This strategy of using the whole capsid precursor protein P1 cDNA for vaccination, intentionally without the use of virus-specific protease or other encoding genes for safety reasons, may thus be employed as a relevant experimental system for induction or upgrading of effective neutralizing antibody response, and as a convenient surrogate test system for DNA vaccination studies of FMDV and presumably other viral diseases.

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Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun

Cited by 2 publications

References 18 publications

Specific microtubule-depolymerizing agents augment efficacy of dendritic cell-based cancer vaccines

Specific microtubule-depolymerizing agents augment efficacy of dendritic cell-based cancer vaccines

Comparative studies of the capsid precursor polypeptide P1 and the capsid protein VP1 cDNA vectors for DNA vaccination against foot‐and‐mouth disease virus

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