Direct ex vivo expansion of hematopoietic stem cells from umbilical cord blood on membranes

Higuchi, Akon; Yang, Siou Ting; Li, Pei Tsz; Tamai, Miho; Tagawa, Yoh‐ichi; Chang, Yung; Yu, Chang; Ling, Qing; Hsu, Shih Tien

doi:10.1016/j.memsci.2010.01.034

Cited by 19 publications

(12 citation statements)

References 28 publications

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“…The human colon carcinoma (HT-29) cell solution or primary colon carcinoma cell solution was filtered through NM filters with r = 11 µm (NM-11) or PGLA-3/SK, PGLA-5/SK, and PGLA-10/SK filters using a batch-type membrane holder (C161, Millipore Corp.) as described previously for blood cell [ 26 , 27 ] or adipose-derived stem cell permeation [ 16 , 28 , 29 , 30 , 31 ]. Figure 1 C shows a schematic representation of a filtration protocol to purify the CICs (CSCs) of colon carcinoma cells from a colon carcinoma cell line (HT-29) and primary colon carcinoma cells.…”

Section: Methodsmentioning

confidence: 99%

Purification of Colon Carcinoma Cells from Primary Colon Tumor Using a Filtration Method via Porous Polymeric Filters

et al. 2021

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Cancer stem cells (CSCs) or cancer-initiating cells (CICs) are key factors for tumor generation and metastasis. We investigated a filtration method to enhance CSCs (CICs) from colon carcinoma HT-29 cells and primary colon carcinoma cells derived from patient colon tumors using poly(lactide-co-glycolic acid)/silk screen (PLGA/SK) filters. The colon carcinoma cell solutions were permeated via porous filters to obtain a permeation solution. Then, the cell cultivation media were permeated via the filters to obtain the recovered solution, where the colon carcinoma cells that adhered to the filters were washed off into the recovered solution. Subsequently, the filters were incubated in the culture media to obtain the migrated cells via the filters. Colon carcinoma HT-29 cells with high tumorigenicity, which might be CSCs (CICs), were enhanced in the cells in the recovered solution and in the migrated cells based on the CSC (CIC) marker expression, colony-forming unit assay, and carcinoembryonic antigen (CEA) production. Although primary colon carcinoma cells isolated from colon tumor tissues contained fibroblast-like cells, the primary colon carcinoma cells were purified from fibroblast-like cells by filtration through PLGA/SK filters, indicating that the filtration method is effective in purifying primary colon carcinoma cells.

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Section: Methodsmentioning

confidence: 99%

Purification of Colon Carcinoma Cells from Primary Colon Tumor Using a Filtration Method via Porous Polymeric Filters

et al. 2021

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“…HSCs are multipotent and have the capability to self-renew and differentiate into all mature blood cells including myeloids such as granulocytes, monocytes, leukocytes, erythrocytes, and megakaryocytes, and lymphoids such as T-lymphocytes and B-lymphocytes ( Figure 2) (Gunsilius, Gastl, & Petzer, 2001;Higuchi et al, 2010). HSCs in UCB are similar to those in BM and peripheral blood in their capacity for differentiation.…”

Section: Hematopoietic Stem Cellsmentioning

confidence: 99%

“…Therefore, a combination of expressed and non-expressed markers are used to identify MSCs. Positive MSC markers include CD29, CD44, CD73 (SH-3, SH-4), CD90 (Thy-1), CD105 (SH-2, Endoglin), and CD166 whereas negative markers include CD11, CD14, CD19, CD31, CD34, CD45, and CD79 (Bieback & Kluter, 2009;Li et al, 2010;Westwood & Clements, 2007).…”

Section: Previous Research Bymentioning

confidence: 99%

Umbilical Cord Blood Hematopoietic Stem Cell Transplantation, an Alternative to Bone Marrow

Najem

Minn

2011

GJHS

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Umbilical cord blood (UCB) is an alternative hematopoietic stem cell (HSC) source. It is widely applicable for ameliorating several diseases through HSC transplants. One of the few disadvantages of this source of stem cells is the limited amount of HSCs that can be extracted from each cord blood unit (CBU).Single CBU transplantation seems to be more ideal in lower weight, younger patients. Adult clinical studies comparing HSCs from a single CBU and HSCs from bone marrow indicate that umbilical cord blood transplantation is a viable method when a matched bone marrow transplant cannot be identified. Further clinical studies using two CBUs suggest better engraftment and lower risk of relapse. However, double cord blood transplantation has been faced with the challenge of single unit dominance in most studies. Ex vivo expansion of UCB HSC is another promising method to overcome limited HSC counts.

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“…Based on current clinical experience, the recommended minimum cell dose numbers for successful CB HSC transplantation are 3.5 × 10 7 total nucleated cells (TNC)/kg and 2 × 10 5 CD34 + HSC/kg [17]. Due to the large number of HSCs needed for transplantation (range 1.5 -14.0 × 10 6 /kg) in clinical applications [18,19], largescale ex vivo expansion systems have captured the interest of many researchers [14,[20][21][22][23]. Bioreactor systems such as perfusion chambers [24], stirred bioreactors [25][26][27], rotating wall bioreactors [28], membrane-based bioreactors [29], and packed or fluidized bed reactors [30,31] have been developed and widely tested for ex vivo expansion of HSCs.…”

Section: Introductionmentioning

confidence: 99%

The hollow fiber bioreactor as a stroma‐supported, serum‐free ex vivo expansion platform for human umbilical cord blood cells

Cao

Kwek

Chan

et al. 2014

Biotechnology Journal

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The bone marrow microenvironment plays an integral role in the regulation of hematopoiesis. Residing stromal cells and the extracellular matrix in the bone marrow microenvironment provide biological signals that control hematopoietic stem cell (HSC) function. In this study, we developed a bio-mimetic co-culture platform using the hollow fiber bioreactor (HFBR) for ex vivo expansion of HSCs. We evaluated the efficacy of such a platform in comparison to standard cultures performed on tissue culture polystyrene (TCP), using a human stromal cell line (HS-5) as stromal support, co-cultured with lineage-depleted human cord blood cells in serum-free medium supplemented with a cytokine cocktail. Our results showed that the performance of the HFBR in supporting total cell and CD34(+) progenitor cell expansion was comparable to that of cultures on TCP. Cells harvested from the HFBR had a higher clonogenic ability. The performance of ex vivo-expanded cells from the HFBR in hematopoietic reconstitution in humanized mice was comparable to that of the TCP control. Scanning electron microscopy revealed that stroma cell growth inside the HFBR created a three-dimensional cell matrix architecture. These findings demonstrate the feasibility of utilizing the HFBR for creating a complex cell matrix architecture, which may provide good in vitro mimicry of the bone marrow, supporting large-scale expansion of HSCs.

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Direct ex vivo expansion of hematopoietic stem cells from umbilical cord blood on membranes

Cited by 19 publications

References 28 publications

Purification of Colon Carcinoma Cells from Primary Colon Tumor Using a Filtration Method via Porous Polymeric Filters

Purification of Colon Carcinoma Cells from Primary Colon Tumor Using a Filtration Method via Porous Polymeric Filters

Umbilical Cord Blood Hematopoietic Stem Cell Transplantation, an Alternative to Bone Marrow

The hollow fiber bioreactor as a stroma‐supported, serum‐free ex vivo expansion platform for human umbilical cord blood cells

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