Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

Sakamoto, Takatoshi; Hasunuma, Tomohisa; Hori, Yutaka; Yamada, R.; Kondo, Akihiko

doi:10.1016/j.jbiotec.2011.06.025

Cited by 124 publications

(70 citation statements)

References 39 publications

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“…Similarly, the problem of post-harvest crop straws can be well solved in this approach. Although a number of practices and studies on how to make use of those crop straws more commercially, for instance, to produce ethanol and the like bio-hydrogen gas [17][ 40], have been attempted, those approaches could not be widely implemented due to the issues of cost effectiveness and how to dispose the process residuals. Up to date, in many places those straws are simply burned in the field after the harvest, which induces serious air pollution and smog problem.…”

Section: The Proposed New Solutionmentioning

confidence: 99%

The W Model, a Systematic Engineering for Water Source Clearance, Renewable Energy Production, and Ecological Agricuture Development – A Proposal for Sustainable Development

Tong-yuan¹

2017

JSD

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This article proposes a systematic engineering for sustainable economic and ecologic development. This system is deemed to be applicable in any country of the world. The system aims to realize five important objectives: water source clearance, energy saving and emission reduction, renewable energy and organic fertilizer production, and ecological agriculture development, all in large scale and at low cost. The main conception of the new system to reach these goals is the replacement of the conventional sewage treatment approach with more efficient and more ecological process -the natural fermentation of the mixture of the urban sewage and agrarian wastes, such that water body clearance, including water de-eutrophication, green algae prevention and siltation dredging will all be accomplished at virtually a zero cost. Along with this process, the system can produce a vast amount of renewable energy and organic fertilizers, consequently ecological agriculture development in large scale can be realized. As a result, this system will greatly reduce the use of chemical fertilizers thus largely reduce the consumption of fossil energy and the related polluting emissions. This system is thus fully a circular economy model through full west-reuse processes, which ultimately will enhance our life quality with healthier food and living environment. The system is flexible and adaptable to be implemented in either small towns or megacities. The implementation and operation of this system will also benefits employment growth. Lastly, in terms of economic feasibility and profitability, millions to billions of dollars of annual revenue can be generated from the running of this system in a country.

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Section: The Proposed New Solutionmentioning

confidence: 99%

The W Model, a Systematic Engineering for Water Source Clearance, Renewable Energy Production, and Ecological Agricuture Development – A Proposal for Sustainable Development

Tong-yuan¹

2017

JSD

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“…Aeling et al (2012) reported that the recombinant S. cerevisiae strain expressing the R. flavefaciens genes encoding xylose isomerase and cellobiose phosphorylase could uptake and assimilate glucose, xylose, and cellobiose under anaerobic conditions (de Haan et al, 2013). Sakamoto et al (2012) developed a recombinant S. cerevisiae strain codisplaying three types of hemicellulolytic enzymes, including endoxylanase from T. reesei, β-xylosidase from A. oryzae, and β-glucosidase from A. aculeatus on the cell-surface. This strain could also assimilate xylose through the expression of xylose reductase and xylitol dehydrogenase from P. stipitis and xylulokinase from S. cerevisiae.…”

Section: -Engineering For Pentose Fermentation By S Cerevisiaementioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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“…BGL gene was integrated to hydrolyze glucooligosaccharide in the hemicellulosic fraction used in this study. Endoxylanase, xylosidase and BGL were displayed on the cell surface of the recombinant yeast strain by fusing each enzyme with the carboxy-terminal domain of α-agglutinin as an anchor region, as previously described [3]. Tolerance to fermentation inhibitors such as acetate, formate and furfural was conferred by integrating the genes for transaldolase (TAL), formate dehydrogenase (FDH), and an alcohol dehydrogenase (ADH) variant (m6ADH1 from S. cerevisiae), into the genome of Sun49-1, yielding Sun49-24.…”

Section: Construction Of Recombinant Yeast Strainsmentioning

confidence: 99%

“…Several bacterial and fungal species can utilize xylan as a carbon source [2], but S. cerevisiae cannot. Thus, many researchers have attempted to produce xylanolytic enzymes in S. cerevisiae strains [3,4]. Furthermore, there has been considerable effort to engineer xylose assimilation pathways in S. cerevisiae [5,6].…”

Section: Introductionmentioning

confidence: 99%

Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses

et al. 2014

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Background: Bioethanol produced by the yeast Saccharomyces cerevisiae is currently one of the most promising alternatives to conventional transport fuels. Lignocellulosic hemicelluloses obtained after hydrothermal pretreatment are important feedstock for bioethanol production. However, hemicellulosic materials cannot be directly fermented by yeast: xylan backbone of hemicelluloses must first be hydrolyzed by heterologous hemicellulases to release xylose, and the yeast must then ferment xylose in the presence of fermentation inhibitors generated during the pretreatment. Results: A GIN11/FRT-based multiple-gene integration system was developed for introducing multiple functions into the recombinant S. cerevisiae strains engineered with the xylose metabolic pathway. Antibiotic markers were efficiently recycled by a novel counter selection strategy using galactose-induced expression of both FLP recombinase gene and GIN11 flanked by FLP recombinase recognition target (FRT) sequences. Nine genes were functionally expressed in an industrial diploid strain of S. cerevisiae: endoxylanase gene from Trichoderma reesei, xylosidase gene from Aspergillus oryzae, β-glucosidase gene from Aspergillus aculeatus, xylose reductase and xylitol dehydrogenase genes from Scheffersomyces stipitis, and XKS1, TAL1, FDH1 and ADH1 variant from S. cerevisiae. The genes were introduced using the homozygous integration system and afforded hemicellulolytic, xylose-assimilating and inhibitor-tolerant abilities to the strain. The engineered yeast strain demonstrated 2.7-fold higher ethanol titer from hemicellulosic material than a xylose-assimilating yeast strain. Furthermore, hemicellulolytic enzymes displayed on the yeast cell surface hydrolyzed hemicelluloses that were not hydrolyzed by a commercial enzyme, leading to increased sugar utilization for improved ethanol production.

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Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

Cited by 124 publications

References 39 publications

The W Model, a Systematic Engineering for Water Source Clearance, Renewable Energy Production, and Ecological Agricuture Development – A Proposal for Sustainable Development

The W Model, a Systematic Engineering for Water Source Clearance, Renewable Energy Production, and Ecological Agricuture Development – A Proposal for Sustainable Development

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses

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