Direct ethanol conversion of pretreated straw by Fusarium oxysporum

A novel ionic liquid-tolerant Fusarium oxysporum BN secreting ionic liquid-stable cellulase: Consolidated bioprocessing of pretreated lignocellulose containing residual ionic liquid

“…A high ethanol yield of 9.3 g/L was achieved using 6% (w/ v) rice straw. The value was comparable to those reported for the similar fungi grown on cellulose as substrate (Christakopoulos et al, 1991). No increase of ethanol production was detected when the rice straw concentration was more than 8% (w/v), which might be due to the higher transfer resistance of substrate in the medium.…”

“…This indicated that cellulases are sufficiently active and hydrolyze cellulose to reducing sugars at this temperature, while the fungus no longer ferments them, resulting in the accumulation of glucose in the culture media. It should mention that the ethanol production by the mesophilic fungus F. oxysporum F3 has been extensively investigated previously (Christakopoulos et al, 1991). Nevertheless, no bibliographic data have been reported so far about the direct bioconversion of IL-pretreated lignocellulose to ethanol based on the wild strains through the I-CBP.…”

Insights from the Fungus Fusarium oxysporum Point to High Affinity Glucose Transporters as Targets for Enhancing Ethanol Production from Lignocellulose

“…For CBP, the shake flask cultivation of delignified straw were conducted as essentially described by Christakopoulos et al [9] with varying length of the oxygen limiting growth phase. Glucose fermentation was carried out in 100 ml conical flask containing 30 ml minimal media [51] supplemented with 10 mM glucose.…”

“…Fungi that have shown promise as CBP agents include Fusarium oxysporum [3], Mucor hiemalis [4], Neurospora crassa [5], Rhizopus oryzae [6] and Trametes hirsute [7]. F. oxysporum has been extensively studied in terms of its ability to produce ethanol from lignocellulosic biomass [3], [8], [9], [10], [11].…”

Challenges in ethanol production withFusarium oxysporumthrough consolidated bioprocessing

“…3 For wheat straw, the ethanol concentration and yield varied between 3 g L ¡1 and 60% and 8 g L ¡1 and 30%, respectively, depending on the concentration of straw in the medium. 15 For untreated wheat bran, an ethanol yield of 34% of the theoretical was achieved, which was increased to 56% with the constitutive expression of a xylanase in F. oxysporum F3. 9 In another study with brewer's spent grain, F. oxysporum never produced more than 9 g L ¡1 ethanol.…”