Direct estimation of oestrone sulphate in sow serum for a rapid pregnancy diagnosis test

Abstract

Summary. An antiserum raised against oestrone-3-glucuronide\p=n-\BSAwas used for the direct radioimmunoassay of oestrone sulphate in pig serum. The method has a minimum sensitivity of 0\m=.\2 ng/ml and is potentially an accurate pregnancy test in pigs. Serum oestrone sulphate concentrations were [ill]0\m=.\5ng/ml in non-pregnant cyclic sows and > 0\m=.\5 ng/ml in pregnant sows bled 26\p=n-\29 days after service.

Changes in oestrone sulphate concentrations in peripheral plasma of Pony mares associated with follicular growth, ovulation and early pregnancy

“…Even when direct measurements of oestrone sulphate have been achieved without prior hydrolysis there have still been up to three extraction steps (Wright, Collins, Musey & Preedy, 1978). However, Saba & Hattersley (1981) In the horse the material which is assayed as LH rises gradually from the time that behavioural oestrus is detected, reaches a peak on or after the day of ovulation and then gradually declines over the next 2-3 days (Whitmore, Wentworth & Ginther, 1973 ;Pattison et al, 1974 ;Noden et al, 1974Noden et al, , 1975. The concomitant measurement of LH and oestrone sulphate will be necessary to elucidate the temporal relationship between these two hormones.…”

Oestrone Sulphate Measurements for the Prediction of Small or Large Litters in Pigs

“…Its conjugates have been demonstrated from day 17 of pregnancy with increasing concentration until day 28-30 and a subsequent decrease later in pregnancy [3,22,23]. The concentration of oestrone and its conjugates in the post-breeding female's plasma, serum, urine, faeces and saliva has been assessed in order to diagnose pregnancy [2,4,10,19,22,24,31]. In addition, a correlation between the number of embryos in early pregnancy and the maternal concentration of oestrone conjugates has been demonstrated [1-3,5,12,30].…”