Direct Enantiomeric Separation of Indapamide by Thin Layer Chromatography using B- Cyclodextrin as Chiral Selector

Abstract

A novel economic thin layer chromatographic method for stereoselective separation of racemic mixture of (RS)-Indapamide and determination of their enantiomers was done. The method was based on using normal TLC plates and  cyclodextrin with hydroxylic part, was used as the chiral selector. Cyclodextrin was used as an additive in silica gel to prepare a TLC plate which was a non-covalent bonding, and there was no chiral substance added in the solvent system. The mobile phase was toluene-ethyl acetate-MeOH-glacial AcOH (6:4:1:0.1). Cyclodextrin was also added to mobile phase, and there was no chiral selector in the stationary phase. The mobile phase was a mixture of toluene-ethyl acetate-MeOH-glacial AcOH – 5mM CD (6:4:1:0.1:1.0). The spots were then isolated and identified. The impact of the componentsofmobile phase, temperature, and pHwas studied for the finding the best separation conditions. The spots were sited in a chamber which had Iodine granules.