Direct Enantiomeric Purity Determination of Acetyl-L-carnitine by LC with a Ligand-Exchange Chiral Stationary Phase

Kagawa, Miyuki; Machida, Yoshio; Nishi, Hiroyuki; Haginaka, Jun

doi:10.1365/s10337-005-0610-z

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…d-AC was not detected in all batches tested (i.e., not more than 0.5%). These results were the same with another analytical data (HPLC method by chiral stationary phases [22]). …”

Section: Validation and Application To The Enantiomeric Purity Determsupporting

confidence: 85%

Enantiomeric purity determination of acetyl-l-carnitine by NMR with chiral lanthanide shift reagents

Kagawa¹,

Machida²,

Nishi³

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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“…d-AC was not detected in all batches tested (i.e., not more than 0.5%). These results were the same with another analytical data (HPLC method by chiral stationary phases [22]). …”

Section: Validation and Application To The Enantiomeric Purity Determsupporting

confidence: 85%

Enantiomeric purity determination of acetyl-l-carnitine by NMR with chiral lanthanide shift reagents

Kagawa¹,

Machida²,

Nishi³

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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“…Several methods, including enantioselective chemical synthesis and transformations of chiral pool substrates, enzymatic kinetic resolutions, biotransformations of prochiral substrates, and resolutions by crystallization of diastereomeric salts have been described in the literature. Fast, accurate, and reproducible analytical methods capable to measure the enantiomeric excess of the final material are also highly desired: in this field it is nice to acknowledge the seminal contribution of Professor Gasparrini and his group . Carnitine and acyl carnitines being chiral molecules, optical rotation (OR) and circular dichroism in the UV range (electronic circular dichroism, ECD) were measured; in the latter case, ECD has been used as a detector of enantiomeric separation, down the line of HPLC separation protocol, without the accuracy required in a dedicated ECD experiment.…”

Section: Methodsmentioning

confidence: 99%

Vibrational Circular Dichroism (VCD) Reveals Subtle Conformational Aspects and Intermolecular Interactions in the Carnitine Family

et al. 2015

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Vibrational circular dichroism spectra (VCD) in the mid-IR region and electronic circular dichroism (ECD) spectra for three carnitine derivatives in the form of hydrochloride salts were recorded in deuterated methanol solutions. Density Functional Theory calculations help one to understand the significance of the observed VCD bands. VCD and ECD spectra are informative about the absolute configuration of the molecule, but VCD data reveal also some conformational aspects in the N,N,N-trimethyl moiety and inform us about intermolecular interactions gained from the carbonyl stretching region for the acyl substituted carnitines.

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“…Further the separation of enantiomers of 8-chloroderivative of HER (clentiazem) was successful by the OVM column [13]. Other than protein immobilized CSPs, enantiomers of TAL were separated by a ligand-exchange type CSP, a CHIRALPAK WH column [9], enantiomers of L-AC were separated by a SUMICHILAL OA-6100 column (4.6 mm ID x 15 cm, 40 o C, flow rate 1.0 mL/min, 254 nm), employing a bulky acid (0.5 mol/L NaClO 4 ) and 2 mmoL/L Cu 2+ as the mobile phase [15] (see, Fig. 3B).…”

Section: Enantiomer Separation Of Pharmaceuticals By Hplc and Its Appmentioning

confidence: 99%

Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis

Nishi

2021

Chromatography

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Pharmaceuticals are essential for the medical treatment of various diseases. From the herbal medicines (crude drugs), synthetic medicines to biopharmaceuticals, to assure its efficacy and safety, the production procedures and the quality control methods of pharmaceuticals are important. These are strictly regulated by Good Manufacturing Practice (GMP) and various guidelines. The key is to develop the "Specifications and testing methods" of the pharmaceutical scientifically and rationally according to the production process. There are many testing items such as identity tests, purity tests, assay, etc. in "Specifications and testing methods". Among them, assay and purity test are core testing items. Optical purity evaluation is required for the single enantiomer pharmaceutical. Chromatographic methods and its detection methods are useful for the purpose. High-performance liquid chromatography (HPLC), thin layer chromatography (TLC) and gas chromatography (GC) are commonly used because the target analyses are almost a mixture. In this review, results of the development of the quality evaluation methods of relatively low molecular pharmaceuticals and herbal medicines by HPLC and capillary electrophoresis (CE) we have been investigated are summarized.

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Direct Enantiomeric Purity Determination of Acetyl-L-carnitine by LC with a Ligand-Exchange Chiral Stationary Phase

Cited by 8 publications

References 15 publications

Enantiomeric purity determination of acetyl-l-carnitine by NMR with chiral lanthanide shift reagents

Enantiomeric purity determination of acetyl-l-carnitine by NMR with chiral lanthanide shift reagents

Vibrational Circular Dichroism (VCD) Reveals Subtle Conformational Aspects and Intermolecular Interactions in the Carnitine Family

Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis

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