Direct embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (� Triticosecale Wittmack) cv. Bogo

Oleszczuk, Sylwia; Sowa, S.; Zimny, J.

doi:10.1007/s00299-004-0796-9

Cited by 53 publications

(55 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, the differences were revealed for haploids and a few aneuploids detected via flow cytometry among regenerants. The level of spontaneously derived DH plants and aneuploids was similar to prior report (Oleszczuk et al 2004). …”

Section: Discussionsupporting

confidence: 75%

See 1 more Smart Citation

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

Machczyńska

Orłowska

Mańkowski

et al. 2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Doubled haploids of triticale are of interest for plant breeders due to hybrid breeding programs based on cytoplasmic male sterility Tt phenomenon. However, (epi)mutations appearing during in vitro culture regeneration may lead to a phenotypic variation that makes the uniformity of plant materials questionable. Using RP-HPLC genomic DNA methylation of donor doubled haploid plants utilized as a source of tissues for the in vitro regeneration (via androgenesis and somatic embryogenesis) of triticale cv. Bogo and their consecutive generative progeny was evaluated. It was demonstrated that in vitro cultures induced a decrease of the DNA methylation of the regenerants independently of the approach used for plant regeneration. The decrease in DNA methylation of genomic DNA proceeded up to the first/second successive generations followed by the beginning of its reestablishment. Moreover, somatic embryogenesis resulted in a higher level of genomic DNA demethylation in regenerants than androgenesis and the process of methylation seems to be affected by donor plant. It is being speculated that long term changes in genomic DNA methylation may be a source of off-type individuals that may spontaneously arise during plant breeding.

show abstract

Section: Discussionsupporting

confidence: 75%

“…Bogo (Oleszczuk et al 2004) were used to generate regenerants via shed microspore culture (M), anther culture (A), and immature zygotic embryo culture (E). The regenerants were used to obtain the first, the second and the third generative progenies.…”

Section: Plant Materialsmentioning

confidence: 99%

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

Machczyńska

Orłowska

Mańkowski

et al. 2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Wanad, used in this study, was earlier successfully used for optimization of procedures for double haploid technology and for identification of physiological parameters important for effective induction of androgenesis (Zur et al, 2008(Zur et al, , 2009. Microspores and structures induced toward androgenic development were observed at 63% frequency, with 2.2-11.4% initial mitotic divisions (Zur et al in preparation, personal communication The morphology resulting from these changes, called star-like structure, has been observed in other species, both dicots such as tobacco (Garrido et al, 1995;Touraev et al, 1996aTouraev et al, , 1997 or rape seed (Zaki and Dickinson, 1990) and monocots such as wheat (Touraev et al, 1996b), barley (de Maraschin et al, 2005 a,b), rice (Bajaj, 1990;Raina and Irfan, 1998), maize (Testillano et al, 2002;Obert et al, 2005) and triticale (Oleszczuk et al, 2004). This star-like morphology has been recognized as the initial step of microspore embryogenic development (Indrianto et al, 1999;Touraev et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Among other steps forward, great progress in isolated microspore culture technique has been made (Pauk et al, 2000;Oleszczuk et al, 2004;Eudes and Amundsen, 2005). Microspore suspension culture of triticale cv.…”

Section: Discussionmentioning

confidence: 99%

Cell Structural Reorganization During Induction of Androgenesis in Isolated Microspore Cultures of Triticale (xTriticosecale Wittm.)

Dubas¹,

Wędzony²,

Petrovská³

et al. 2010

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

Upon stress treatment, isolated microspores of triticale (×Triticosecale Wittm.) were directed towards sporophytic development (androgenesis). We used fluorescence microscopy to study the cell structural reorganization associated with the process. Changes in the developmental pathway coincided with the character of the microtubular cytoskeleton configuration, the number and direction of nuclear divisions, changes in vacuolization, the distribution of mitochondria, ER and starch grains, and the architecture of new cell wall formation. A band of diffused fluorescence surrounding the nucleus was observed before the first symmetric division of microspores. This structure most likely represents a preprophase band (PPB). Successive mitotic divisions within the microspore wall led to the formation of multinucleate or multicellular structures consisting of one or two domains of cells differing in size. They were later released from the sporoderm and continued further development with features typical for a monocotyledonous embryo. The pattern of internal architecture of androgenic structures depended on their developmental phase. Before and after release from the microspore wall, cortical microtubules (MTs) exhibited various configurations without preferential orientation. They formed a denser network in the region opposite to the sporoderm rupture site. Released multicellular structures showed both intensely fluorescing cortical MTs and more dispersed endoplasmic MTs radiating along the cytoplasmic strands from the nuclear region to the cell cortex. Up to globular stage, isotropically expanding cells of androgenic embryos showed a random pattern of MTs. This is the first report that successive events of androgenic development of triticale microspores are associated with MT reorganization. The results support the view that changes in cytoskeleton architecture are critical during induction of androgenesis.K Ke ey y w wo or rd ds s: : Androgenesis, microspores, triticale, cytoskeleton, microtubules.

show abstract

“…The range of questions associated with in vitro cultures of interspecific and integeneric hybrids is very wide and diverse [87][88][89][90]. These questions have been given much attention because of their significant practical implications, such as broadening of the species' genetic variability, creation of new gene combinations and the production of haploids.…”

Section: Culture Of Zygotic and Hybrid Embryosmentioning

confidence: 99%

65 years of in vitro culture in Poland

Zenkteler

2013

Acta Soc Bot Pol

View full text Add to dashboard Cite

This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements

show abstract

Direct embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (� Triticosecale Wittmack) cv. Bogo

Cited by 53 publications

References 36 publications

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

Cell Structural Reorganization During Induction of Androgenesis in Isolated Microspore Cultures of Triticale (xTriticosecale Wittm.)

65 years of in vitro culture in Poland

Contact Info

Product

Resources

About