Direct electrotransfer of hHGF gene into kidney ameliorates ischemic acute renal failure

Franquesa, M.; Alperovich, Gabriela; Herrero‐Fresneda, Immaculada; Lloberas, Núria; Bolaños, Núria; Fillat, Cristina; Rama, Inés; Cruzado, Josep M.; Grinyó, Josep M.; Torrás, Joan

doi:10.1038/sj.gt.3302569

Cited by 33 publications

(26 citation statements)

References 49 publications

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“…In vivo electroporation has been shown to be effective for a wide range of tissues, including tumours (Rols et al 1998;Li 2008), skin (Marti et al 2004;Medi and Singh 2008), liver (Jaichandran et al 2006;Kamimura and Liu 2008), lung (Dean et al 2003;Zhou et al 2007), kidney (Franquesa et al 2005), thymus (Irla et al 2008), bladder (Yoshida et al 2008), adipose tissue (Granneman 2008), vasculature (Matsumoto et al 2001), retina (Matsuda and Cepko 2008), cornea (Blair-Parks et al 2002;Oshima et al 2002), ciliary muscle (Bloquel et al 2006), brain (Boutin et al 2008), spinal cord (Wu et al 2004;Chen et al 2008), skeletal muscle (McMahon and Wells 2004;Miyazaki and Miyazaki 2008;Brown et al 2008) and testis (Parrington et al 2007;Dhup andMajumdar 2008, Yomgogida 2008). A range of genetic material has been used: DNA, RNA and oligonucleotides (e.g.…”

Section: In Vivo Electroporationmentioning

confidence: 97%

Electroporation and ultrasound enhanced non-viral gene delivery in vitro and in vivo

Wells

2009

Cell Biol Toxicol

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Non-viral vectors are less efficient than the use of viral vectors for delivery of genetic material to cells in vitro and especially in vivo. However, viral vectors involve the use of foreign proteins that can stimulate both the innate and acquired immune response. In contrast, plasmid DNA can be delivered without carrier proteins and is non-immunogenic. Plasmid gene delivery can be enhanced by the use of physical methods that aid the passage of the plasmid through the cell membrane. Electroporation and microbubble-enhanced ultrasound are two of the most effective physical delivery methods and these can be applied to a range of different cell types in vitro and a broad range of tissues in vivo. Both techniques also have the advantage that, unlike viral vectors, they can be used to target specific tissues with systemic delivery. Although electroporation is often the more efficient of the two, microbubble-enhanced ultrasound causes less damage and is less invasive. This review provides an introduction to the methodology and summarises the range of cells and tissues that have been genetically modified using these techniques.

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Section: In Vivo Electroporationmentioning

confidence: 97%

Electroporation and ultrasound enhanced non-viral gene delivery in vitro and in vivo

Wells

2009

Cell Biol Toxicol

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“…Several studies have shown that HGF administration to rodents accelerates tubular regeneration and functional recovery after acute kidney injury (AKI) [5,6,7]. More recently, HGF transgenic or transfected mice showed resistance to AKI, particularly to tubular cell apoptosis, suggesting a protective role for HGF in the development of AKI [8,9,10]. Finally, recent papers have shown that HGF has the capacity to reduce the inflammatory reaction in chronic renal disease models [11, 12].…”

Section: Introductionmentioning

confidence: 99%

Endogenous Hepatocyte Growth Factor Attenuates Inflammatory Response in Glycerol-Induced Acute Kidney Injury

et al. 2008

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Background: Hepatocyte growth factor (HGF) is overexpressed after acute kidney injury (AKI). The aim of this study was to evaluate the role of endogenous HGF in the progression of the inflammatory response in glycerol-induced AKI (Gly-AKI) in rats. Methods: Renal and systemic HGF expressions were evaluated during the development of Gly-AKI. Subsequently, the blockade of endogenous HGF was analyzed in rats treated with anti-HGF antibody concomitant to glycerol injection. Apoptosis, cell infiltration and chemokine and cytokine profiles were investigated. Results: We detected an early peak of renal and plasma HGF protein expressions 3 h after glycerol injection. The pharmacological blockade of the endogenous HGF exacerbated the renal impairment, the tubular apoptosis, the renal expression of monocyte chemoattractant protein-1 and the macrophage, CD43+, CD4+ and CD8+ T lymphocytes renal infiltration. The analysis of mRNA expressions of Th1 (t-bet, TNF-α, IL-1β) and Th2 (gata-3, IL-4, IL-10) cytokines showed a Th1-polarized response in Gly-AKI rats that was aggravated with the anti-HGF treatment. Conclusion: Endogenous HGF attenuates the renal inflammatory response, leukocyte infiltration and Th1 polarization after glycerol injection. The control of cellular immune response may partly explain the protective effect of endogenous HGF in the development of Gly-AKI.

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“…Most in vivo studies also showed that HGF treatment reduced the magnitude of acute tubular necrosis 1 or 2 days after AKI induction, but the mitotic indexes were increased in the HGF-treated animals [10, 11]; accelerated repair instead of reduced necrosis could be responsible for this finding [31,32,33,34]. de Souza Durão et al [35] showed direct evidence that HGF can reduce apoptosis and necrosis in Madin-Darby canine kidney cells subjected to hypoxia.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies utilizing different methods of treatment with HGF, either in vivo or in renal epithelial cell culture, consistently showed the antiapoptotic effect of HGF after tubular injuries [15, 16,29,30,31,32,33]. Most in vivo studies also showed that HGF treatment reduced the magnitude of acute tubular necrosis 1 or 2 days after AKI induction, but the mitotic indexes were increased in the HGF-treated animals [10, 11]; accelerated repair instead of reduced necrosis could be responsible for this finding [31,32,33,34].…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Glycerol-Induced Acute Kidney Injury by Previous Partial Hepatectomy: Role of Hepatocyte Growth Factor/c-met Axis in Tubular Protection

Homsi

Janino²,

Biswas³

et al. 2007

Nephron Exp Nephrol

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Background/Aims: Previous partial hepatectomy (HPTX) can attenuate glycerol-induced acute kidney injury (Gly-AKI). The aim of this study was to explore the pathophysiological mechanisms and the role of hepatocyte growth factor (HGF) in kidney protection. Methods: Rats were subjected to HPTX 24 h before glycerol administration. Renal function, acute tubular necrosis, apoptosis, leukocyte infiltration, and the expression of HGF, c-met, monocyte chemoattractant protein-1, interleukin-1β, and heme oxygenase-1 were evaluated 24 h after glycerol injection. The regenerative response was analyzed from 6 to 72 h after glycerol injection (BrdU incorporation). In a separate series of experiments, Gly-AKI+HPTX rats were treated with anti-HGF antibody. Results: Gly-AKI+HPTX rats showed an increased expression of renal HGF and c-met as well as an improved creatinine clearance and reduced acute tubular necrosis and apoptosis, cytokine expression, and leukocyte infiltration. The regenerative response was less intense 24 and 72 h after glycerol administration in this group. The anti-HGF treatment disclosed an important role of HGF in the reduction of tubular injury, particularly apoptosis. Overexpression of heme oxygenase-1 was observed in Gly-AKI+HPTX rats, but was not associated with HPTX-induced renal protection. Conclusion: We conclude that Gly-AKI+HPTX rats have a reduced susceptibility to renal injury instead of an increased regenerative response and that endogenous HGF overexpression is responsible for suppression of tubular apoptosis.

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Direct electrotransfer of hHGF gene into kidney ameliorates ischemic acute renal failure

Cited by 33 publications

References 49 publications

Electroporation and ultrasound enhanced non-viral gene delivery in vitro and in vivo

Electroporation and ultrasound enhanced non-viral gene delivery in vitro and in vivo

Endogenous Hepatocyte Growth Factor Attenuates Inflammatory Response in Glycerol-Induced Acute Kidney Injury

Attenuation of Glycerol-Induced Acute Kidney Injury by Previous Partial Hepatectomy: Role of Hepatocyte Growth Factor/c-met Axis in Tubular Protection

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