Direct electron transfer catalyzed by bilirubin oxidase for air breathing gas-diffusion electrodes

Gupta, Gautam; Lau, Carolin; Rajendran, Vijaykumar; Colon, Frisia; Branch, Brittany; Ivnitski, Dmitri; Atanassov, Plamen

doi:10.1016/j.elecom.2010.12.024

Cited by 96 publications

(65 citation statements)

References 41 publications

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“…[175,323,324] Sugar/airbreathing EBFCs were recently developed and helped to overcome this issue, because oxygen is brought as a gas and not as a dissolved species. [218,[325][326][327] In view of the removal of the membrane separator in H 2 /O 2 EBFCs, mixtures of H 2 and O 2 must be controlled outside their explosive limits. This requires a search for enzymes that display high affinity toward their substrates.…”

Section: Chemelectrochem Reviewsmentioning

confidence: 99%

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Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

et al. 2014

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Among sustainable alternatives to fossil fuel, proton exchange membrane fuel cells are promising devices that deliver electricity from hydrogen and oxygen, producing only water. The transformation of the fuel and oxidant however relies on rare‐metal catalysts. H2/O2 enzymatic biofuel cells thus emerge as sustainable biotechnological devices in which chemical catalysts are replaced by hydrogenases at the anode and multicopper oxidases at the cathode. This review discusses the recent breakthroughs and the limitations in all the components of H2/O2 biofuel cells: 1) Catalytic mechanisms involved in multicopper oxidases and hydrogenases, in addition to the new potential of enzymes from the biodiversity pool, which exhibit outstanding properties. 2) The molecular basis for oriented enzyme immobilization on the electrochemical interfaces as a prerequisite for a fast direct electron‐transfer rate. 3) The requirement for 3D networks to enhance current densities. 4) The production of H2 from biomass. 5) The history of H2/O2 biofuel cells and recent devices, which also highlights the remaining issues that will allow the use of such devices in low‐power applications.

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Section: Chemelectrochem Reviewsmentioning

confidence: 99%

“…Carbon black materials have been successfully used for BOD immobilization. [62,218,219] Carbon NPs were assembled by a layer-by-layer approach and used for Aa MbH encapsulation. [220] Graphite particles were also decorated by E. coli Hase.…”

Section: Carbon Nanomaterialsmentioning

confidence: 99%

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

et al. 2014

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“…Recently, we also demonstrated the development of gas-diffusion cathodes for oxygen reduction based on adsorptive immobilization of laccase and bilirubin oxidase. [20,21] Carbon nanotubes (CNTs) are known for their extraordinary electrical and mechanical properties, [22] high surface area, low resistivity, and chemical stability, that together makes them an interesting material for various applications in microelectronics, composite materials, and electrical applications. On the molecular scale, the CNTs walls consist of sp 2 graphene carbon atoms with delocalized π-electrons which provide perfect conjugation sites for other highly aromatic molecules.…”

Section: Design Of Carbon Nanotube-based Gas-diffusion Cathode For O mentioning

confidence: 99%

Design of Carbon Nanotube‐Based Gas‐Diffusion Cathode for O₂ Reduction by Multicopper Oxidases

Lau

Adkins

Ramasamy

et al. 2011

Advanced Energy Materials

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Multicopper oxidases, such as laccase or bilirubin oxidase, are known to reduce molecular oxygen at very high redox potentials, which makes them attractive biocatalysts for enzymatic cathodes in biological fuel cells. By designing an enzymatic gas‐diffusion electrode, molecular oxygen can be supplied through the gaseous phase, avoiding solubility and diffusion limitations typically associated with liquid electrolytes. In doing so, the current density of enzymatic cathodes can theoretically be enhanced. This publication presents a material study of carbon/Teflon composites that aim to optimize the functionality of the gas‐diffusion and catalytic layers for application in enzymatic systems. The modification of the catalytic layer with multiwalled carbon nanotubes, for example, creates the basis for stronger π–π stacking interactions through tethered enzymatic linkers, such as pyrenes or perylene derivates. Cyclic voltammograms show the effective direct electron contact of laccase with carbon nanotube‐modified electrodes via tethered crosslinking molecules as a model system. The polarization behavior of laccase‐modified gas‐diffusion electrodes reveals open‐circuit potentials of +550 mV (versus Ag/AgCl) and current densities approaching 0.5 mA cm2 (at zero potential) in air‐breathing mode.

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“…In fact, it seems feasible to use the cuticle of certain insects as the electrode support which would avoid adding extra weight. Yet another factor that would improve the performance metrics would entail changes in the overall architecture of the enzymatic cathode involving structures that resemble air permeable electrodes 16 for conventional fuel cells. Efforts in this direction are being pursued in several laboratories including ours.…”

Section: Concluding Remarks and Future Outlookmentioning

confidence: 99%

Wireless Communication by an Autonomous Self-Powered Cyborg Insect

Schwefel

Ritzmann

Lee

et al. 2014

J. Electrochem. Soc.

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A trehalose/oxygen biofuel cell was implanted in Blaberus discoidalis to convert chemical energy stored within the insect hemolymph into electrical energy which was then used to power a custom-designed oscillator mounted on the back of the insect, capable of producing signals in the audible range. The ability of this cyborg to generate and transmit signals wirelessly was demonstrated by placing an external receiver up to a few centimeters away from the insect while it was tethered to a device that enabled it to walk in place on top of a light weight, air-suspended solid sphere. Wireless communication could also be established between the transmitter powered by the same type of biofuel cell implanted in the moth Manduca sexta and the receiver, while the live insect was being restrained with wax in a Petri dish. Possible means of reducing the weight and size of the transmitter so as to allow the moth to carry it in flight are discussed. Biofuel cells1,2 have emerged as promising devices for converting chemical into electrical energy in living organisms with potential application in a variety of areas of fundamental and technical relevance.1-6 Efforts in our laboratories have focused on the development of biofuel cells that could be implanted into insects 6 and provide the power required not only for the operation of electronics for sensing, information storage and wireless communication, but also for the stimulation of the nervous system, a strategy that will ultimately allow control of certain aspects of the insect behavior. An attractive feature of this approach is that it provides a continuous and autonomous source of power thereby avoiding the need for an external battery as implemented recently by Bozkurt et al., 7 who succeeded in controlling wirelessly the path of motion of a different species of cockroach Gromphadorhina portentosa. To this end, we designed, constructed and successfully tested an implantable biofuel cell incorporating a bienzymatic anode capable of dissociating trehalose, 8 a dissacharide found in very high concentrations, up to tens of mM, in the hemolymph of insects, 9 to yield glucose, which is then oxidized to gluconolactone by the enzyme glucose oxidase. As shown recently in our laboratories, this type of biofuel cell can generate up to 15.6 μW/cm 2 when implanted in a live cockroach Blaberus discoidalis.6 This contribution represents an extension of our earlier studies and is aimed at demonstrating that a single biofuel cell implanted in the insect can power a judiciously designed electronic oscillator mounted on the insect capable of sending wirelessly signals that can be captured by an external receiver, while the insect, tethered to a fixed structure, is allowed to walk on an air-suspended Styrofoam sphere. ExperimentalElectrochemistry.-Enzyme-modified electrodes were prepared by depositing either a mixture of glucose oxidase (Aspergillus niger, EC 1. pressed and purified in our laboratory), PVI-(dmbpy) 2 Cl 2 (10 mg/mL), and PEGDGE (2.5 mg/mL) (Polysciences, Inc.) where PVI denot...

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Direct electron transfer catalyzed by bilirubin oxidase for air breathing gas-diffusion electrodes

Cited by 96 publications

References 41 publications

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Design of Carbon Nanotube‐Based Gas‐Diffusion Cathode for O₂ Reduction by Multicopper Oxidases

Wireless Communication by an Autonomous Self-Powered Cyborg Insect

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Direct electron transfer catalyzed by bilirubin oxidase for air breathing gas-diffusion electrodes

Cited by 96 publications

References 41 publications

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective

Design of Carbon Nanotube‐Based Gas‐Diffusion Cathode for O2 Reduction by Multicopper Oxidases

Wireless Communication by an Autonomous Self-Powered Cyborg Insect

Contact Info

Product

Resources

About

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Biohydrogen for a New Generation of H₂/O₂ Biofuel Cells: A Sustainable Energy Perspective

Design of Carbon Nanotube‐Based Gas‐Diffusion Cathode for O₂ Reduction by Multicopper Oxidases