Direct electron transfer and sensing performance for catechin of nano-gold particles-polymer nano-composite with immobilized Laccase

Yang, Yaru; Zeng, Han; Zhang, Q.; Bai, Xiaoxue; Liu, C.; Zhang, Y.H.

doi:10.1016/j.cplett.2016.06.063

Cited by 26 publications

(8 citation statements)

References 20 publications

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“…In 2016, catechin was also determined using an electrode covered by nano-composite with laccase by Zeng et al [ 91 ] ( Figure 9 ). The nano-composite is a hybrid of chitosan-g-N-carboxymethyl-2-sulfo-4, 5-2H imidazolinone and gold nanoparticles tailored with 4-mercaptobenzoic acid, and is used as enzyme carrier to prepare glassy carbon electrode with entrapped laccase.…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

Biosensors and Sensing Systems for Rapid Analysis of Phenolic Compounds from Plants: A Comprehensive Review

2020

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Phenolic compounds are secondary metabolites frequently found in plants that exhibit many different effects on human health. Because of the relevant bioactivity, their identification and quantification in agro-food matrices as well as in biological samples are a fundamental issue in the field of quality control of food and food supplements, and clinical analysis. In this review, a critical selection of sensors and biosensors for rapid and selective detection of phenolic compounds is discussed. Sensors based on electrochemistry, photoelectrochemistry, fluorescence, and colorimetry are discussed including devices with or without specific recognition elements, such as biomolecules, enzymes and molecularly imprinted materials. Systems that have been tested on real matrices are prevalently considered but also techniques that show potential development in the field.

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Section: Electrochemical Sensorsmentioning

confidence: 99%

Biosensors and Sensing Systems for Rapid Analysis of Phenolic Compounds from Plants: A Comprehensive Review

2020

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“…Because they possess ortho dihydroxy or ortho trihydroxy structure, catechins could form half quinone free radicals when they act on free radicals, leading to harmful oxygen free radical inactivation [20]. Chelate reaction of catechins with oxidizing metal ions could inhibit the oxidation activity of metal ions due to its ortho hydroxyl phenol structure [21,22]. The versatility of catechins and their active metabolites as potential therapeutic interventions are due to the diverse actions being performed at different sites.…”

Section: Catechinsmentioning

confidence: 99%

Preventive Effects of Catechins on Cardiovascular Disease

Chen

Han

et al. 2016

Molecules

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Abstract:Catechins are polyphenolic phytochemicals with many important physiological activities that play a multifaceted health care function in the human body, especially in the prevention of cardiovascular disease. In this paper, various experimental and clinical studies have revealed the role of catechins in the prevention and treatment of cardiovascular disorders, and we review the preventive effects of catechins on cardiovascular disease from the following aspects: Regulating lipid metabolism, regulating blood lipid metabolism, vascular endothelial protection, and reducing blood pressure.

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“…Although the stability of the immobilized enzyme is improved by traditional immobilization methods, the activity was usually lower than that of the free enzyme. This is due to the complex immobilization process and limited mass transfer, resulting in loss of enzyme activity. , To resolve these issues, many new technologies have been proposed for enzyme immobilization. , Recently, organic–inorganic hybrid nanoflowers with specific structure and better performance are the rapidly expanding fields of novel immobilized enzyme technologies. , …”

Section: Introductionmentioning

confidence: 99%

“…22,23 To resolve these issues, many new technologies have been proposed for enzyme immobilization. 24,25 Recently, organic−inorganic hybrid nanoflowers with specific structure and better performance are the rapidly expanding fields of novel immobilized enzyme technologies. 26,27 In 2012, Ge et al first reported a method for preparing enzyme−inorganic hybrid nanoflowers using a-lactalbumin, laccase, carbonic anhydrase, and lipase as the organic component and copper (II) ions as the inorganic component, which showed much greater activities than free enzymes and most of the reported immobilized enzymes.…”

Section: Introductionmentioning

confidence: 99%

Construction of Multienzyme Co-immobilized Hybrid Nanoflowers for an Efficient Conversion of Cellulose into Glucose in a Cascade Reaction

Han

Feng

et al. 2021

J. Agric. Food Chem.

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Today, we are seeking an efficient biotransformation of cellulosic material into sustainable biochemical products to meet the increasing global energy demand. Herein, we report the fabrication of multienzyme hybrid nanoflowers (ECG-NFs) by co-immobilizing three recombinant enzymes (cellobiohydrolase (CBH), endo-glucanase (EG), and β-glucosidase (BG)) integrating a binary tag composed of elastin-like polypeptide (ELP) and His-tag to act as a tri-enzyme biocatalyst, which catalyzes the hydrolysis of cellulose into glucose. The prepared ECG-NFs exhibited excellent performance in terms of pH stability, thermal stability, storage stability, and catalytic efficiency compared to free multienzyme system. Notably, ECG-NFs could be recycled for up to eight consecutive runs. The K m and k cat/K m values for ECG-NFs were 9.33 g L–1 and 0.0051 L min–1 g–1, respectively, which were better than those of the free multienzyme system, indicating a better substrate affinity. Finally, the overall enzyme activity of ECG-NFs increased by 1.12 times and the degradation efficiency of ECG-NFs was superior to the free multienzyme system, which revealed that ECG-NFs could facilitate an effective one-pot hydrolysis of cellulose into glucose.

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Direct electron transfer and sensing performance for catechin of nano-gold particles-polymer nano-composite with immobilized Laccase

Cited by 26 publications

References 20 publications

Biosensors and Sensing Systems for Rapid Analysis of Phenolic Compounds from Plants: A Comprehensive Review

Biosensors and Sensing Systems for Rapid Analysis of Phenolic Compounds from Plants: A Comprehensive Review

Preventive Effects of Catechins on Cardiovascular Disease

Construction of Multienzyme Co-immobilized Hybrid Nanoflowers for an Efficient Conversion of Cellulose into Glucose in a Cascade Reaction

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