Direct electrochemistry of glucose oxidase immobilized on Au nanoparticles-functionalized 3D hierarchically ZnO nanostructures and its application to bioelectrochemical glucose sensor

Fang, Linxia; Liu, Bing; Liu, Lulu; Li, Yuehua; Huang, Ke‐Jing; Zhang, Qiuyu

doi:10.1016/j.snb.2015.08.032

Cited by 120 publications

(46 citation statements)

References 34 publications

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“…The annealed nano powder showed peaks that can be indexed to the hexagonal wurtzite structure ZnO, which coincide with the standard JCPDS card (202) plane [37]. The strong sharp diffraction peak indicate that ZnO nanoparticles annealed at 500°C are well crystallized [6,31]. The crystallite size of ZnO nanoparticles were calculated from peak broadening of diffraction peak using Scherrer formula [9, 17,38,39].…”

Section: Electrochemistrymentioning

confidence: 62%

“…Thus, showing the CV for bare GCE and ZnO nanoparticles at different scan rates [35,48]. The shape of the curves is nearly rectangular show clear increase in current with scan rate which suggests the contribution from faradaic reaction [15,31]. It is further interesting to note that CV curves remain unchanged as scan rate increase thus indicating the excellent electrochemical reversibility and exceptional high rate performance [49].…”

Section: Electrochemical Studymentioning

confidence: 89%

“…[1,2,30]. In the biological synthesis method, the use of plant extracts, fungi, microbes, and raw materials of fruits and vegetables are used for synthesis of metal and metal oxide NPs [26,31,32]. The various types of NPs have been synthesized by using this green and eco-friendly way using plants extract and microbes [22].…”

Section: Introductionmentioning

confidence: 99%

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The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

Mayedwa¹,

Khalil²,

Mongwaketsi³

et al. 2017

Nano Res Appl

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In this contribution an exceptionally simple, cost effective and reliable method for biosynthesis of ZnO nanoparticles through using Sageretia thea natural extracts as an effective chelating agent. The morphological analysis shows ZnO has nanoscale particles size 28.09 nm ± 5 nm, crystalline and possess distinct nanostructure hexagonal wurtzite structure. Effects of heat on ZnO synthesised at room temperature caused bonds with in the molecule to be broken exhibited continuous weight loss with 3 quasi sharp changes and 3 endothermic peaks occurring at 79°C, 228°C and 300°C. A proposed mechanism of reaction for Zn(NO 3 ) 2 6H 2 O precursor with bioactive compounds in Sageretia thea extract such as phenolic acid, flavonoids, natural sterolin and vitamin based compounds to form ZnO nanoparticles. Electrochemically ZnO nanoparticles are electroactive by exhibiting charge transfer resistance (R ct ) 82006 Ω and bare GCE 3.7707 × 10 5 Ω. Showed good catalytic and conductivity which can be applied for pseudo capacitors.

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Section: Electrochemistrymentioning

confidence: 62%

Section: Electrochemical Studymentioning

confidence: 89%

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The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

Mayedwa¹,

Khalil²,

Mongwaketsi³

et al. 2017

Nano Res Appl

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“…However, as the redox active group of glucose oxidase (GOx), flavin adenine dinucleotide (FAD), is deeply embedded within protein shell, accomplishment of DET is quite difficult, and only the certain materials allow DET . These materials include graphene (G)‐based materials, carbon nanotubes, quantum dots, metal nanoparticles and metal oxide nanoparticles such as TiO 2 , ZnO, CeO 2 , and conducting polymers . Graphene is a one atom‐thick advanced material consisting of sp 2 ‐bonded carbon with honeycomb structure, and so far, graphene and its derivatives have been extensively utilized as electrode materials, and have conspicuous advantages for the development of biosensors such as, decent electrical conductivity, high surface area to volume ratio, ability to bind substances onto their surfaces, and were employed in the researches extensively over the last decade .…”

Section: Introductionmentioning

confidence: 94%

Tannic Acid Modified Electrochemical Biosensor for Glucose Sensing Based on Direct Electrochemistry

Çakıroğlu

Özacar

2017

Electroanalysis

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A novel glucose biosensor was constructed through the immobilization of glucose oxidase (GOx) on gold nanoparticles (Au NPs) deposited, and chemically reduced graphene oxide (rGO) nanocomposite. In the synthesis, tannic acid (TA) was used for the reduction of both graphene oxide, and Au3+ to rGO, and Au NPs, respectively. Also, by harnessing the π‐π interaction between graphene oxide and TA, and protein‐TA interaction, a novel nanocomposite for the fabrication of a third generation biosensor was successfully constructed. Upon the oxidation of TA to quinone, which is easily reducible at the negative potential range, enhanced electron transfer was obtained. The cyclic voltammetry (CV) results demonstrated a pair of well‐defined and quasi‐reversible redox peaks of active site molecule of GOx. The biosensor exhibited a linear response to glucose concentrations varying from 2 to 10 mM with a sensitivity of 18.73 mA mM−1 cm−2. The fabricated biosensor was used for the determination of glucose in beverages.

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“…Binding of glucose oxidase to several solids including polymers, 25,26 graphene nanocomposites, 27 and gold nanoparticle–zinc oxide nanostructures has been examined. 28 GOx was tested for applications in biofuel cells 29,30 and sensors 31–34 and for oxidations using ambient oxygen. For example, GOx catalyzes the oxidation of glucose to gluconic acid while reducing oxygen to hydrogen peroxide.…”

Section: Introductionmentioning

confidence: 99%

Tuning Enzyme/α-Zr(IV) Phosphate Nanoplate Interactions via Chemical Modification of Glucose Oxidase

et al. 2017

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Using glucose oxidase (GOx) and α-Zr(IV) phosphate nanoplates (α-ZrP) as a model system, a generally applicable approach to control enzyme–solid interactions via chemical modification of amino acid side chains of the enzyme is demonstrated. Net charge on GOx was systematically tuned by appending different amounts of polyamine to the protein surface to produce chemically modified GOx(n), where n is the net charge on the enzyme after the modification and ranged from −62 to +95 electrostatic units in the system. The binding of GOx(n) with α-ZrP nanosheets was studied by isothermal titration calorimetry (ITC) as well as by surface plasmon resonance (SPR) spectroscopy. Pristine GOx showed no affinity for the α-ZrP nanosheets, but GOx(n) where n ≥ −20 showed binding affinities exceeding (2.1 ± 0.6) × 106 M−1, resulting from the charge modification of the enzyme. A plot of GOx(n) charge vs Gibbs free energy of binding (ΔG) for n = +20 to n = +65 indicated an overall increase in favorable interaction between GOx(n) and α-ZrP nanosheets. However, ΔG is less dependent on the net charge for n > +45, as evidenced by the decrease in the slope as charge increased further. All modified enzyme samples and enzyme/α-ZrP complexes retained a significant amount of folding structure (examined by circular dichroism) as well as enzymatic activities. Thus, strong control over enzyme–nanosheet interactions via modulating the net charge of enzymes may find potential applications in biosensing and biocatalysis.

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Direct electrochemistry of glucose oxidase immobilized on Au nanoparticles-functionalized 3D hierarchically ZnO nanostructures and its application to bioelectrochemical glucose sensor

Cited by 120 publications

References 34 publications

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

Tannic Acid Modified Electrochemical Biosensor for Glucose Sensing Based on Direct Electrochemistry

Tuning Enzyme/α-Zr(IV) Phosphate Nanoplate Interactions via Chemical Modification of Glucose Oxidase

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