Direct Electrochemistry of Glucose Oxidase Immobilized on Titanium Carbide‐Au Nanoparticles‐Fullerene C₆₀ Composite Film and Its Biosensing for Glucose

Abstract

The direct electrochemistry of glucose oxidase (GOD) immobilized on the designed titanium carbide-Au nanoparticles-fullerene C 60 composite film modified glassy carbon electrode (TiC-AuNPs-C 60 /GCE) and its biosensing for glucose were investigated. UV-visible and Fourier-transform infrared spectra of the resulting GOD/TiC-AuNPs-C 60 composite film suggested that the immobilized GOD retained its original structure. The direct electron transfer behaviors of immobilized GOD at the GOD/TiC-AuNPs-C 60 /GCE were investigated by cyclic voltammetry in which a pair of well-defined, quasi-reversible redox peaks with the formal potential (E 0¢ ) of -0.484 V (vs. SCE) in phosphate buffer solution (0.05 M, pH 7.0) at the scan rate of 100 mV·s -1 were obtained. The proposed GOD modified electrode exhibited an excellent electrocatalytic activity to the reduction of glucose, and the currents of glucose reduction peak were linearly related to glucose concentration in a wider linearity range from 5.0 × 10 -6 to 1.6 × 10 -4 M with a correlation coefficient of 0.9965 and a detection limit of 2.0 × 10 -6 M (S/N = 3). The sensitivity and the apparent Michaelis-Menten constant (K M app ) were determined to be 149.3 mA·mM -1 ×cm -2 and 6.2 × 10 -5 M, respectively. Thus, the protocol will have potential application in studying the electron transfer of enzyme and the design of novel electrochemical biosensors. CHEMICAL SOCIETYFig. 4. Cyclic voltammograms of GOD/TiC-AuNPs-C 60 /GCE in 0.05 M N 2 -saturated PBS (pH 7.0) at various scan rates (from inner to outer curve): 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0 V·s -1 . Inset graph: peak current of the cathodic and anodic vs. scan rate. 1010 www.jccs.wiley-vch.de

Fullerene-derived nanocomposite as an efficient electrocatalyst for overall water splitting and Zn–air battery

“…53 Wang et al immobilized the novel glucose sensor prepared by glucose oxidase (GOD) on the TiC-AuNPs-C 60 composite by a reduction reaction and ultrasonic stirring. 57 The SEM images of GOD/TiC-AuNPs-C 60 /GCE showed that the TiC-AuNPs-C 60 composite film was still compact and uniform, and the particle size increased significantly, indicating that C 60 had been successfully synthesized into the TiC-AuNPs composite material.…”

Ti3C2Tx MXene as Electrocatalyst for Designing Robust Glucose Biosensors

“…The corresponding sensitivity and the apparent Michaelis-Menten constant (K M app ) were 149.3 µA mM −1 cm −2 and 62 × 10 −6 m, respectively. [82] Using conducting polymers in combination with sensor materials is another way to improve the conductivity and stability of the manufactured sensor. PEDOT:PSS, or poly (3,4ethylenedioxythiophene) polystyrene sulfonate was explored to improve the conductivity of electrodes with a modest band gap, good stability, and low redox potential.…”

“…The corresponding sensitivity and the apparent Michaelis–Menten constant ( K M app ) were 149.3 µA mM −1 cm −2 and 62 × 10 −6 m , respectively. [ 82 ]…”

Hydrogen-bonding 2D coordination polymer for enzyme-free electrochemical glucose sensing

“…[1][2][3][4][5] Due to the intrinsic properties of enzymes, the performance of enzyme-based sensors depends on pH, temperature and humidity. [6][7][8][9][10][11][12] Therefore, non-enzymatic systems for glucose detection have been proposed, and are regarded as being more useful under a wider range of conditions and more readily adaptable to device fabrication. [13][14][15][16][17][18] In enzyme-free systems, redox couples such as FeIJII)/FeIJIII) are used for catalyzing the transformation of glucose into gluconic acid.…”