Direct Electrochemistry of Glucose Oxidase on Nail‐like Carbon and Its Biosensing for Glucose

Li, Kuangtian; Liu, Bin; Zheng, Jianbin; Sheng, Qinglin; Liu, Ruixiao

doi:10.1002/elan.200900419

Cited by 12 publications

(5 citation statements)

References 49 publications

(26 reference statements)

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“…The ratio of cathodic to anodic current intensity is about 1, indicating that a fast direct electron transfer reaction occurred. The value of E°′ was close to the values reported previously [ 35 , 36 ]. According to the equation I p = n 2 F 2 vAΓ */4 RT = nFQv /4 RT [ 37 ], the surface concentration of electroactive Γ * was calculated to be 2.34 × 10 −12 mol/cm 2 , which matched the theoretical value (2.86 × 10 −12 mol/cm 2 ) for the monolayer of GOx on the bare electrode surface, indicating that the AuNPs/GOx–PGR/GCE keep the nanostructure of GR.…”

Section: Resultssupporting

confidence: 91%

Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene

Zheng

Wang

et al. 2017

Materials

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Controllable preparation of nanopores to promote the performance of electrochemical biosensing interfaces has become one of the researching frontiers in biosensing. A double biocatalysis signal amplification of glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using horseradish peroxidase biosynthesized porous graphene (PGR) as the platform for the biocatalytic deposition of gold nanoparticles (AuNPs). The biosensor showed a linear range from 0.25 to 27.5 μM with a detection limit of 0.05 μM (S/N = 3) towards glucose. Furthermore, the proposed AuNPs/GOx–PGR modified glassy carbon electrode (AuNPs/GOx–PGR/GCE) achieved direct electron transfer of GOx.

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Section: Resultssupporting

confidence: 91%

Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene

Zheng

Wang

et al. 2017

Materials

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“…The value of E 00 was similar to that obtained at the Nafion/GOx/C-ZnO electrode 44 and the GOx-NLC-CHIT/GCE. 45 According to the equation of 46 the surface concentration of electroactive G* was calculated to be 3.16 Â 10 À9 mol cm À2 . This value was much larger than that of the theoretical value (2.86 Â 10 À12 mol cm À2 ) for the monolayer of GOx on the bare electrode surface.…”

Section: Direct Electrochemistry and Electrocatalysis Of Goxmentioning

confidence: 99%

DNA as a linker for biocatalytic deposition of Au nanoparticles on graphene and its application in glucose detection

Zheng

Sheng

et al. 2011

J. Mater. Chem.

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Taking advantages of the striking properties of both self-assembly and biocatalysis, a highly sensitive glucose electrochemical biosensor was proposed by using DNA-GE as biocatalysis target-guide to deposit Au nanoparticles (AuNPs). AuNPs interacted with thiol and amino groups of DNA strands, which make the reaction much easier and faster. Furthermore, the proposed AuNPs/glucose oxidase (GOx)/DNA-GE/glassy carbon (GC) modified electrode achieved the direct electrochemistry and electrocatalysis of GOx. The growth of AuNPs was confirmed by scanning electron microscopy and electrochemical methods. The characterizations of the electrode modified after each assembly step and the content of AuNPs on the electrode surfaces during the growth process were investigated by cyclic voltammetry. The amount of AuNPs was relative to the amount of glucose oxidized accompanying with the biocatalytic process of GOx. The biosensor showed a linearity with glucose concentration in the range of 0.8-50 mM with a detection limit of 0.3 mM (S/N ¼ 3). The sensitivity was 2.4 Â 10 4 mA mM À1 . The combination of self-assembly and biocatalysis offers the new design of enzymatic biosensors with potential applications in direct electrochemistry and biocatalysis.

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“…Interestingly, Gray et al some time ago reported on the direct wiring of the topaquinone (TPQ) cofactor deeply buried in Arthrobacter globiformis amine oxidase (AGAO) protein to Au-bead electrodes modified by the diethylaniline-terminated oligo(phenylethynyl)thiol (DEA-OPE-SH) SAMs (note at least 8 Å van der Waals diameter for the “safely penetrating” diethylaniline terminal group). Application of much thinner (“naillike”) carbon-based materials (see, e.g., ref ), obviously, may provide potentially much less damaging methodology for direct wiring in the future. Furthermore, the narrower character of CV peaks for GOx compared to those of the isolated FAD, already discussed above, is an additional strong evidence in favor of the CNT direct penetration into the central interior part of GOx, such to provide the nearly equal possibility of ET from/to both FADs residing inside GOx (this statement does not imply that both FADs transfer electrons simultaneously, but rather behave just as statistically independent entities positioned in an almost similar position vs the CNT wire).…”

Section: Resultsmentioning

confidence: 99%

New Evidence for a Quasi-Simultaneous Proton-Coupled Two-Electron Transfer and Direct Wiring for Glucose Oxidase Captured by the Carbon Nanotube–Polymer Matrix

et al. 2015

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Systematic cyclic voltammetry (CV) studies of glucose oxidase (GOx) and its cofactor, flavine adenine dinucleotide (FAD), almost similarly captured by the matrix of single-walled carbon nanotube and polymer complex, in turn, deposited on GC electrodes have been performed. The comparative analysis of kinetic data obtained for the FAD and GOx specimens treated through the same Marcus theory-based algorithmic procedure strongly suggests that the GOx species, notwithstanding the deeply buried position of FAD, mechanistically behave virtually in the same manner as isolated FADs (the operationally capable, nearly intact structure of GOx was confirmed by the catalytic activity vs glucose), strongly suggesting that FADs inside GOx are directly wired to the GC electrode, presumably, via almost direct contact of nanotubes with both FADs residing inside each GOx biomolecule (as basically suggested by Guiseppi-Elie, A.; et al. Nanotechnology 2002, 13, 559−564, and shortly supported by a number of valued researchers). Furthermore, the nonadiabatic, quasi-simultaneous two-proton-coupled two-electron transfer/exchange mechanism was concluded from further cross-analysis based on a generalized Marcus theory for the proton-coupled electron transfer (PCET), extra furnished by the first-time temperature-dependent CV studies and a subsequent Arrhenius treatment.

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Direct Electrochemistry of Glucose Oxidase on Nail‐like Carbon and Its Biosensing for Glucose

Cited by 12 publications

References 49 publications

Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene

Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene

DNA as a linker for biocatalytic deposition of Au nanoparticles on graphene and its application in glucose detection

New Evidence for a Quasi-Simultaneous Proton-Coupled Two-Electron Transfer and Direct Wiring for Glucose Oxidase Captured by the Carbon Nanotube–Polymer Matrix

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