Direct electrochemistry of cytochrome c at a novel gold nanoparticles-attached ions implantation-modified indium tin oxide electrode

Li, Shuoqi; Xia, Jianling; Liu, Chenyao; Cao, Wei; Hu, Jingbo; Li, Qilong

doi:10.1016/j.jelechem.2009.06.006

Cited by 17 publications

(11 citation statements)

References 60 publications

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“…In recent years, the electrochemical immunoassays have been developed based on nanomaterials, such as colloid gold (Xiao & Yu, 2010), nano-SiO 2 (Tang, Su, Tang, Ren & Chen, 2010;Wu, Chen & Liu, 2009), nano-TiO 2 (Wang, Ruan, Kanayeva, Lassiter & Li, 2008), nano-Ag (Loyprasert et al, 2008), carbon nanotube (Diakowski, Xiao, Petryk & Kraatz, 2010), Fe 3 O 4 nanoparticles (Huang et al, 2010), and nano-Pt (Huang, Wen, Jiang, Shen & Yu, 2008), all of which have attracted considerable interest in biosensor development. Among the nanomaterials mentioned above, gold nanoparticles, which have good characteristics of easy preparation, good biocompatibility and relatively large surface, are widely used to immobilize DNA (Li, Feng, Dong & Tang, 2010;Zhang, Wang & Xu, 2010), cell (Yan, Chen & Ju, 2007), cytochrome (Bonk & Lisdat, 2009;Li et al, 2009), and enzyme (Kim, Kang, Shim & Moon, 2008;Wang, Wang, Di & Tu, 2008) in the field of biological studies. Recently, gold nanoparticles have been used to immobilize the antigen or antibody onto the electrode surface to manufacture the electrochemical immunosensors (Valera, Muñiz & Rodríguez, 2010;.…”

Section: Introductionmentioning

confidence: 99%

Detecting 5-morpholino-3-amino-2-oxazolidone residue in food with label-free electrochemical impedimetric immunosensor

Jin

Yang

et al. 2011

Food Control

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Section: Introductionmentioning

confidence: 99%

Detecting 5-morpholino-3-amino-2-oxazolidone residue in food with label-free electrochemical impedimetric immunosensor

Jin

Yang

et al. 2011

Food Control

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“…Surprisingly, no efforts are being made to modify the electronic properties of SnO 2 by implanting it with suitable metals that have multiple oxidation states especially for detection of uric acid. Amongst different metals, copper (Cu) has multi-electron oxidation states and is attractive due to natural abundance, low cost, non-toxic and good electrochemical activity [6,14]. The impantation of Cu in SnO 2 may induce the redox property besides enhancing the electron transfer property for possible biosensing application.…”

Section: Introductionmentioning

confidence: 99%

Modified Tin Oxide Based Bioelectrode for Reagentless Detection of Uric Acid

Arora¹,

Tomar²,

Gupta³

2017

J Biosens Bioelectron

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A reagentless uric acid biosensor has been realized using Copper implanted tin oxide thin film (Cu:SnO 2 ) based matrix. The biosensing characteristics of implanted matrix have been studied using the electrochemical impedance spectroscopy and cyclic voltammetry. The prepared matrix (Cu:SnO 2 ), because of the presence of Cu, possess redox properties so that the electron transfer from enzyme to the electrode could be accomplished without using any external mediator in the electrolyte. The developed uric acid biosensor exhibits a high sensitivity of about 0.93 mA/ mM and a linear variation in current response over a concentration range from 0.05 to 1.0 mM of uric acid besides high shelf life (~20 weeks). The Michaelis-Menten kinetic parameter (K m ) is found to be relatively very low (0.12 mM), which indicates the high affinity of the fabricated bioelectrode towards the uric acid (analyte). The results highlight the importance of implanted Cu:SnO 2 thin film as an attractive matrix for the realization of reagentless biosensors towards uric acid.

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“…The morphology and the structure of AuNPs were characterized by SEM that the diameters were between 5 nm and 15 nm, while the average radius is 10 nm in our previous works [37,38]. In order to further study electrochemical application of this new electrode material, we chose cytochrome c as redox protein to fabricate the modified electrode.…”

Section: Introductionmentioning

confidence: 99%

ITO electrode modified by a gold ion implantation technique for direct electrocatalytic sensing of hydrogen peroxide

et al. 2012

Self Cite

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We report on a simple strategy for the fabrication of gold nanoparticles (AuNPs) on an indium tin oxide substrate using a modified ion implantation method. The morphology, structure and electrochemical features of AuNPs were characterized by atomic force microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode has a large electrochemically active surface and enables strong loading with cytochrome c (Cyt c) proteins. It undergoes enhanced electron transfer at uncompromised electrochemical activity, and also displays good stability and repeatability. The immobilized Cyt c exhibits good electrocatalytic activity towards hydrogen peroxide (H 2 O 2 ), with a linear relationship between the catalytic current during differential pulse voltammetry and the concentration of H 2 O 2 in the 0.05 μM to 0.2 μM range. The detection limit (S/N 0 3) is 0.01 μM.

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Direct electrochemistry of cytochrome c at a novel gold nanoparticles-attached ions implantation-modified indium tin oxide electrode

Cited by 17 publications

References 60 publications

Detecting 5-morpholino-3-amino-2-oxazolidone residue in food with label-free electrochemical impedimetric immunosensor

Detecting 5-morpholino-3-amino-2-oxazolidone residue in food with label-free electrochemical impedimetric immunosensor

Modified Tin Oxide Based Bioelectrode for Reagentless Detection of Uric Acid

ITO electrode modified by a gold ion implantation technique for direct electrocatalytic sensing of hydrogen peroxide

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