Direct Electrochemistry and Electrocatalysis of Myoglobin Immobilized on Gold Nanoparticles/Carbon Nanotubes Nanohybrid Film

Abstract

A novel nanohybrid material, constructed by gold nanoparticles (GNPs) and multiwalled carbon nanotubes (MWNTs), was designed for immobilization and biosensing of myoglobin (Mb). Morphology of the nanohybrid film was characterized by SEM. UV-vis spectroscopy demonstrated that Mb on the composite film could retain its native structure. Direct electrochemistry of Mb immobilized on the GNPs/MWNTs film was investigated. The immobilized Mb showed a couple of quasireversible and well-defined cyclic voltammetry peaks with a formal potential of about À 0.35 V (vs. Ag/AgCl) in pH 6.0 phosphate buffer solution (PBS) solution. Furthermore, the modified electrode also displayed good sensitivity, wide linear range and long-term stability to the detection of hydrogen peroxide. The experiment results demonstrated that the hybrid matrix provided a biocompatible microenvironment for protein and supplied a necessary pathway for its direct electron transfer.

Rapid microwave-assisted synthesis and electrochemical characterization of gold/carbon nanotube composites

“…However, when the gold electrode modified with Au/CNT composites, the redox peak decreases a lot but is also higher than that of bare gold electrode (curve 4 in Fig. 2e), which is similar to the reports [33,34]. According to the report [34], it is attributed to the distribution of Au NPs with negative charge on the surface of CNTs to hinder the diffusion of ferricyanide toward the electrode surface.…”

Simultaneous detection of ascorbic acid, dopamine, uric acid and tryptophan with Azure A-interlinked multi-walled carbon nanotube/gold nanoparticles composite modified electrode

“…Surface-linked Au-CNT nanocomposites may further be classified as covalently linked and non-covalently linked (Zhang and Olin, 2011). For non-covalent bonding, there are several ways to attach linking molecules to carbon nanotubes, including p stacking (Chen et al, 2008;Liu et al, 2003;Wang et al, 2002, Wang and Bi, 2013Ou and Huang, 2006;Wang et al, 2007), hydrophobic forces (Richard et al, 2003;Cao et al, 2008;Xiao et al, 2008;Alexeyeva et al, 2006), and electrostatic interactions (Zhang and Olin, 2011;Jiang et al, 2003;Yao and Shiu, 2008). CNT/nanoparticle hybrid materials, in which nanoparticles are attached to CNT surfaces, have been reported to exhibit catalytic activity, enhanced electrical conductivity, and hydrogen-sensing capability, suggesting broad potential application in optoelectronics, molecular sensors and heterogeneous catalysis.…”

Gold-carbon nanotube nanocomposites: synthesis and applications

“…Zhang et al (2006a) fabricated Au-CNT nanohybrids by using SDS, which hydrophobically adsorbed on carbon nanotube and mediated the attachment of gold nanoparticles. Similar methods can be found in other papers, where the nanocomposites were used for high sensitive chemical and biological sensing (Xiao et al, 2008;Cao et al, 2008;Alexeyeva et al, 2006), as well as in an application to measure the total length of carbon nanotubes using an 'electrochemical ruler' (Streeter et al, 2008).…”