Direct electrochemistry and electrocatalysis of hemoglobin on an indium tin oxide electrode modified with implanted carboxy ions

Porous Polystyrene-Block-Poly(Acrylic Acid)/Hemoglobin Membrane Formed by Dually Driven Self-Assembly and Electrochemical Application

“…The formal potential was found to be linearly decreased with increasing pH from 4.5 to 8.5 (a-i) with a slope of −50.7 mV• pH −1 (Inset A of Figure 8). This value was considered to be close to the theoretical value of −57.6 mV•pH −1 for a reversible proton-coupled single ET, 39 and suggested that the ET between the electrode and a heme group of an Hb was a process accompanied by single protonation, which can be represented in a general term as…”

“…The formal potential was found to be linearly decreased with increasing pH from 4.5 to 8.5 (a-i) with a slope of −50.7 mV·pH -1 (Inset A of Figure 8). This value was considered to be close to the theoretical value of −57.6 mV·pH -1 for a reversible proton-coupled single ET 39 , and suggested that the ET between the electrode and a heme group of 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 14 an Hb was a process accompanied by single protonation, which can be represented in a general term as where the charges on Hb species were not labeled. …”

Biocatalyst and Colorimetric/Fluorescent Dual Biosensors of H2O2 Constructed via Hemoglobin–Cu3(PO4)2 Organic/Inorganic Hybrid Nanoflowers

“…Notably, the peaks corresponding to Fe 2p were not found, indicating that more active centers can be exposed in the organic/inorganic hybrid structure. The N 1s spectra of Hb–Cu 3 (PO 4 ) 2 HNFs can be separated into C–N/N–H (399.62 eV) and N–CO (400.0 eV) (Figure d), belongs to Hb molecular . The C 1s XPS spectrum of Hb–Cu 3 (PO 4 ) 2 HNF was decomposed into four components, assigned to C–C/C–H (284.6 eV), C–S/C–N (286.1 eV), amide (287.7 eV), and carboxyl (289.7 eV) groups of the Hb molecules (Figure e). , The XPS analysis also found the binding energies of P 2p at 131.15 eV corresponding to the characteristic of PO 4 3– (Figure f) …”

Voltammetric Determination of Hemoglobin Using a Pencil Lead Electrode

“…Therefore, with electrochemical immobilization protocol, a small amount of Hb could be immobilized on the electrode surface. Thus, nonelectrochemically immobilization of Hb was used by most scientists for investigation of the Hb behavior on different electrode surfaces 6, 37, 38, 41–43. On the basis of the obtained results, despite of production of a higher oxidation signal in the nonelectrochemical immobilization protocol, the reproducibility of this strategy in determination of low concentrations of Hb was rather poor.…”