Direct Electrochemical Interaction between a Modified Gold Electrode and a Bacterial Membrane Extract

Jeuken, Lars J. C.; Connell, Simon D.; Nurnabi, Mohammed; O’Reilly, John; Henderson, Peter J. F.; Evans, Stephen D.; Bushby, Richard J.

doi:10.1021/la047732f

Cited by 49 publications

(60 citation statements)

References 40 publications

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“…Impedance data obtained for the system can be very well fitted to the equivalent circuit proposed in other reports for a SLB (e.g. [32]) suggesting the formation of the latter. The equivalent circuit used for fitting the experimental data is R s (R m CPE1)CPE2, where the terms within brackets are parallel.…”

Section: Resultssupporting

confidence: 68%

Ubiquinone-10 in gold-immobilized lipid membrane structures acts as a sensor for acetylcholine and other tetraalkylammonium cations

Mårtensson

Hernández

2012

Bioelectrochemistry

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This is an accepted version of a paper published in Bioelectrochemistry. This paper has been peer-reviewed but does not include the final publisher proof-corrections or journal pagination.Citation for the published paper: Mårtensson, C., Agmo Hernańdez, V. (2012 AbstractIt is reported that the reduction of ubiquinone incorporated into supported lipid bilayers and into immobilized liposome layers on gold electrodes is kinetically and thermodynamically enhanced by the presence of acetylcholine and tetrabutylammonium (TBA + ) in solution. The reduction peak and the mid-peak potentials of the redox reactions, determined by cyclic voltammetry, are displaced towards more positive potentials by approximately 500 and 250 mV, respectively, in the case of TBA + ; and by approximately 750 and 530 mV, respectively, in the case of acetylcholine. The intensity of the signal varies with the cation concentration, allowing for quantitative determinations in the millimolar range. It is proposed that the enhanced reduction of ubiquinone arises from the formation of tetraalkylammonium cationubiquinone radical anion ion-pairs. Electrochemical quartz crystal microbalance with dissipation monitoring (EQCM-D) measurements confirmed that the potential shift and the intensity of the redox signal are coupled with the adsorption of the tetraalkylammonium cations on the lipid membrane. The Langmuir adsorption equilibrium constant (K) of TBA + on lipid membranes at physiological pH is determined. In supported lipid bilayers K = 440.7 ± 160 M -1 , while in an immobilized liposome layer K = 35.53 ± 3.53 M -1 . 1

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Section: Resultssupporting

confidence: 68%

Ubiquinone-10 in gold-immobilized lipid membrane structures acts as a sensor for acetylcholine and other tetraalkylammonium cations

Mårtensson

Hernández

2012

Bioelectrochemistry

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“…They have shown that the presence of Ca 2+ strongly promotes SLB formation in particular on TiO 2 while also leading to the asymmetric distribution of lipids between the two leaflets. 13,14,16,[25][26][27][28][29] The main motivation for membrane research--including research using SLB--has been applications in drug screening, which has resulted in less attention to surface functionalization with membranes mimicking other organisms than eukaryote cells ͑cf. the references above͒.…”

Section: Introductionmentioning

confidence: 99%

“…Some previous attempts have been made toward creating SLB from liposomes including some relevant lipids for bacteria [29][30][31][32][33][34][35][36][37][38] or even close to complete bacterial lipid mixtures. 27,28,30,31,39,40 However, these studies have mostly used hydrophobic or polycationic polymers, which strongly promote liposome rupture in order to improve SLB yield, which resulted in incomplete and probably strongly pinned SLB, or else are low coverage preparations of SLB and vesicles on mica and glass substrates There is also lacking an investigation of how these membranes are formed, and thus little knowledge for improving coverage and reproducibility of the SLB. Biosensors functionalized with lipid membranes mimicking bacteria would be a valuable tool given the increasing interest in devising new and understanding biologically derived antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Formation of supported bacterial lipid membrane mimics

Merz¹,

Knoll²,

Textor³

et al. 2008

Biointerphases

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In recent years, a large effort has been spent on advancing the understanding of how surface-supported membranes are formed through vesicle fusion. The aim is to find simple model systems for investigating biophysical and biochemical interactions between constituents of cell membranes and, for example, drugs and toxins altering membrane function. Designing and controlling the self-assembly of model membranes onto sensor substrates thus constitutes an important field of research, enabling applications in, e.g., drug screening, dynamic biointerfaces, artificial noses, and research on membrane-active antibiotics. The authors have developed and investigated the formation of strongly negatively charged supported lipid membranes which systematically mimic bacterial membrane composition on three important biosensor materials: SiO2, TiO2, and indium tin oxide. By tuning the electrostatic interaction through balancing the lipid vesicle charge with the ionic strength of Ca2+ as a fusion promoter, the authors have optimized the self-assembly and obtained new insights into the details of lipid vesicle-surface interaction. The results will be useful for future development and application of specialized lipid membrane surface coatings prepared from complex lipid compositions. The adsorption processes were characterized by a quartz crystal microbalance with dissipation monitoring, optical waveguide lightmode spectroscopy, and fluorescence recovery after photobleaching, which allowed the determination of formation also of nonplanar supported lipid membranes.

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“…30 However, most cellular membranes are composed largely of protein ͑for example, bacterial membranes consist of as much as 50-60% protein 31,32 ͒, which poses a problem when attempting to form a SBLM. A recent study by Graneli et al 33 detailing bilayer formation with proteoliposomes highlighted that extramembranous domains of membrane proteins hinder vesicle rupture.…”

Section: Introductionmentioning

confidence: 99%

Native E. coli inner membrane incorporation in solid-supported lipid bilayer membranes

et al. 2008

Self Cite

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Solid-supported bilayer lipid membranes (SBLMs) containing membrane protein have been generated through a simple lipid dilution technique. SBLM formation from mixtures of native Escherichia coli bacterial inner membrane (IM) vesicles diluted with egg phosphatidylcholine (egg PC) vesicles has been explored with dissipation enhanced quartz crystal microbalance (QCM-D), atomic force microscopy (AFM), attenuated total internal-reflection Fourier-transform infrared spectroscopy (ATR-FTIR), and fluorescence recovery after photobleaching (FRAP). QCM-D studies reveal that SBLM formation from vesicle mixtures ranging between 0% and 100% IM can be divided into two regimes. Samples with ≤40% IM form SBLMs, while samples of greater IM fractions are dominated by vesicle adsorption. FRAP experiments showed that the bilayers formed from mixed vesicles with ≤40% IM were fluid, and comprised a mixture of both egg PC and IM. ATR-FTIR measurements on SBLMs membranes formed with 30% IM confirm that protein is present. SBLM formation was also explored as a function of temperature by QCM-D and FRAP. For samples of 30% IM, QCM-D data show a decreased mass and viscoelasticity at elevated temperatures, and an increased fluidity is observed by FRAP measurements. These results suggest improved biomimetic characteristics can be obtained by forming and maintaining the system at, or close to, 37 °C.

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Direct Electrochemical Interaction between a Modified Gold Electrode and a Bacterial Membrane Extract

Cited by 49 publications

References 40 publications

Ubiquinone-10 in gold-immobilized lipid membrane structures acts as a sensor for acetylcholine and other tetraalkylammonium cations

Ubiquinone-10 in gold-immobilized lipid membrane structures acts as a sensor for acetylcholine and other tetraalkylammonium cations

Formation of supported bacterial lipid membrane mimics

Native E. coli inner membrane incorporation in solid-supported lipid bilayer membranes

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