Direct Electrochemical DNA Sensor based on a new redox oligomer modified with ferrocene and carboxylic acid: Application to the detection of Mycobacterium tuberculosis mutant strain

Bizid, S.; Blili, Saber; Mlika, R.; Saı̈d, Ayoub Haj; Korri-Youssoufi, Hafsa

doi:10.1016/j.aca.2017.09.022

Cited by 28 publications

(8 citation statements)

References 36 publications

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“…The poly(p-phenylene) (PPP) modified with ferrocene groups (Fc), as side chains (Fc-PPP) has been synthesized and characterized by NMR and FTIR as described previously [30], the poly(p-phenylene) (PPP) modified with ferrocene groups (Fc) and carboxylic acids (Ac) as functional groups in side chains (Fc-ac-PPP) has been prepared by the alkaline hydrolysis of the Fc-PPP and confirmed by FTIR spectra as described previously [31].…”

Section: Reagentsmentioning

confidence: 99%

“…The Fc-ac-PPP and Fc-PPP solutions have been prepared by dispersing 5 mg of each polymer in 1.5 mL CHCl 3 , and ultra-sonicated for 30 min. The gold surface, after polishing steps, was dropped with an optimum amount of 5 µl Fc-ac-PPP or Fc-PPP solution as optimized previously [31] followed by a drying step under ambient temperature during 30 min. After the evaporation of the volatile solvent, the modified gold surface was then rinsed with distilled water to remove non-attached molecules.…”

Section: Surface Preparation and Modificationmentioning

confidence: 99%

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Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample

et al. 2020

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Electrochemical sensor for the individual and the simultaneous detection of dopamine (DA), ascorbic acid (AA) and uric acid (UA) based on redox conjugated "poly(paraphenylene)" (Fc-ac-PPP) bearing ferrocene and carboxylic acid in lateral position has been developed. The electrochemical characterization of the sensor has been studied with cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperometry (CA). We highlighted that the catalytic activity of the Fc-ac-PPP polymer provided by it's redox electrochemical properties and chemical structure allows to the electrochemical detection of DA, AA and UA. We demonstrated that the sensor provides high sensitivity and selective signal in the coexistence of DA, AA and UA within short time. Low detection limits and wide linear ranges of detection have been demonstrated respectively for DA 3×10 -10 M (1nM-10µM), AA 1.6×10 -8 M (0.1 µM -1mM), and UA 1×10 -8 M (0.1µM-1mM). In addition, the sensor has been successfully applied to determine DA in urine and human serum samples even in the presence of high concentrations of AA and UA. This sensor could be a powerful device for the detection of other electroactive compounds thanks to it's high catalytic properties and chemical structure.

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Section: Reagentsmentioning

confidence: 99%

Section: Surface Preparation and Modificationmentioning

confidence: 99%

Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample

et al. 2020

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“…The membranes based on the nanocomposite CRGO/Fc-ac-PPP with different mass ratio were characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) to underline the best ratio for sensor application. The polymer intituled "poly(para-phenylene)" modified with ferrocene and an acidic group in its backbone (Fc-ac-PPP) has been synthesized and characterized according to the previously reported procedure [26,27]. Gold electrode, graphene oxide (GO), dimethylformamide (DMF), ethanol, phosphate buffer solution (PBS tablets), AA, DA, UA, potassium ferrocyanide ([Fe(CN)6] 3-/4-), ruthenium hexamine ([Ru(NH3)6] 2+/3+ ) and urine samples were purchased from Sigma Aldrich (France).…”

Section: Nanocomposite Formation Crgo/fc-ac-pppmentioning

confidence: 99%

Nanocomposite Based on Poly (para-phenylene)/Chemical Reduced Graphene Oxide as a Platform for Simultaneous Detection of Ascorbic Acid, Dopamine and Uric Acid

Hsine

Bizid

Mlika

et al. 2020

Sensors

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In this study, an efficient and simple designed nanohybrid created for individual and simultaneous detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA). This nanohybrid is a combination of chemical reduced graphene oxide (CRGO) and redox poly(para-phenylene) (Fc-ac-PP) modified in a lateral position with ferrrocenyl group CRGO/Fc-ac-PPP. The CRGO/Fc-ac-PPP nanohybrid demonstrated a synergistic effect resulting in a large conductivity, surface area and catalytic properties provided by the redox attached ferrocene. Moreover, this nanocomposite is able to detect individually as well as simultaneously AA, DA and UA in a co-existence system with defined and separated redox peaks oxidation. The linear response ranges for AA, DA and UA, when detected simultaneously, are 0.1–10000 μM, 0.0001–1000 μM and 0.1–10000 μM, respectively, and the detection limits (S/N = 3) are 0.046 μM, 0.2 nM and 0.013 μM, respectively. The proposed sensor shown satisfactory results when applied to real spiked urine samples for measuring the abnormal high or lowconcentration of AA, DA and UA in vivo.

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“…This outcome is attributed to the large surface area of silica nanospheres (SiNSs) for DNA immobilisation sites. When compared with DNA hybridisation indicator that employed ferrocenium compounds 9,10 , the biosensor developed here has comparable LOD, but it did not require any procedure for ferrocene labelling, i.e. chemical attachment of ferrocene compounds to the DNA.…”

Section: The Percentage Of Hypochromicity Was Calculated Following Eqmentioning

confidence: 99%

“…Sato and Takenaka 8 studied electrochemical DNA detection using ferrocenylnaphthalenediimide. In most of the reported work involving ferrocene/Fe(III) compounds of DNA hybridisation indicator, they were used as labels that needed to be chemically attached to the DNA probes to detect a hybridisation event 9,10 .…”

Section: Introductionmentioning

confidence: 99%

Hexaferrocenium Tri [Hexa(Isothiocyanato)Iron(III)] trihydroxonium Complex as a New DNA Intercalator for Electrochemical DNA Biosensor

Ariffin

Zakariah

Ruslin

et al. 2020

Preprint

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Ferrocene or ferrocenium has been widely studied in the field of organometallic complexes because of its stable thermodynamic, kinetic and redox properties. Novel Hexaferrocenium Tri[Hexa(Isothiocyanato)Iron (III)]Trihydroxonium (HexaFc) complex was the product from the reaction of ferrocene, maleic acid and ammonium thiocyanate and it was confirmed by elemental analysis CHNS, FTIR and single crystal x-ray crystallography. In this study, HexaFc was used for the first time as an electroactive indicator for DNA hybridisation biosensor. The UV-Vis DNA titrations with this compound showed hypochromism and redshift at 250 nm with increasing DNA concentrations. The binding constant (Kb) for HexaFc complex towards CT-DNA (calf-thymus DNA) was 3.1 × 104 M−1. These results indicated intercalator behaviour of the complex. To test the usefulness of this complex for DNA biosensor application, a porcine DNA biosensor was constructed. The recognition probes were covalently immobilised onto silica nanospheres (SiNS) via glutaraldehyde linker on a screen-printed electrode (SPE). After intercalation with the complex, the biosensor response to complementary porcine DNA was measured using differential pulse voltammetry. The DNA biosensor demonstrated a linear response range to the complementary porcine DNA from 1 × 10−6 to 1 × 10−3 µM (R2 = 0.9642) with a limit detection of 4.83 × 10−8 µM. The results indicated that HexaFc complex is a feasible indicator for DNA hybridisation evaluation without the need of chemical attachment as a label.

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Direct Electrochemical DNA Sensor based on a new redox oligomer modified with ferrocene and carboxylic acid: Application to the detection of Mycobacterium tuberculosis mutant strain

Cited by 28 publications

References 36 publications

Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample

Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample

Nanocomposite Based on Poly (para-phenylene)/Chemical Reduced Graphene Oxide as a Platform for Simultaneous Detection of Ascorbic Acid, Dopamine and Uric Acid

Hexaferrocenium Tri [Hexa(Isothiocyanato)Iron(III)] trihydroxonium Complex as a New DNA Intercalator for Electrochemical DNA Biosensor

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Direct Electrochemical DNA Sensor based on a new redox oligomer modified with ferrocene and carboxylic acid: Application to the detection of Mycobacterium tuberculosis mutant strain

Cited by 28 publications

References 36 publications

Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample

Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample

Nanocomposite Based on Poly (para-phenylene)/Chemical Reduced Graphene Oxide as a Platform for Simultaneous Detection of Ascorbic Acid, Dopamine and Uric Acid

Hexaferrocenium Tri [Hexa(Isothiocyanato)Iron(III)] trihydroxonium Complex as a New DNA Intercalator&nbsp;for Electrochemical DNA Biosensor

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Hexaferrocenium Tri [Hexa(Isothiocyanato)Iron(III)] trihydroxonium Complex as a New DNA Intercalator for Electrochemical DNA Biosensor