Direct electrocatalytic and simultaneous determination of purine and pyrimidine DNA bases using novel mesoporous carbon fibers as electrocatalyst

Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

“…In the absence of dsDNA, no obvious peak was seen during the potential scan. After the addition of dsDNA, two concentration-dependent irreversible oxidation peaks appear at about +0.6 V and +0.9 V. This corresponds to the known pH-dependent mechanism of electrochemical oxidation of G-and A-residues, which are present in dsDNA [8]. The rather large potential gap of 0.3 V between these peaks points out that the electrochemical responses of G-and Aresidues do not interfere.…”

“…Electroanalysis of heterocyclic bases, nucleotides, DNA and RNA (direct electrochemistry of DNA bases) is based on the appearance of individual oxidative peaks of purines: guanine (G) and adenine (A) (typically at < 1.0 V potentials) and pyrimidines: cytosine (C) and thymine (T) (at higher positive potentials), following a pH-dependent mechanism of electrooxidation [14]. For heterocyclic bases taken at equal concentrations, however, the currents corresponding to electrooxidation of pyrimidines (T and C) are lower compared to the ones for electrooxidation of purines (A and G) [8].…”

Electrochemical Methods for Studies of Biological Molecules

“…Было показано, что использование стеклоуглеродных рабочих электродов, модифицированных мезопористыми углеродными волокнами, смещает потенциал окисления по сравнению с немодифицированным электродом, что даёт возможность зарегистрировать электроокисление не только пуриновых оснований А и G, но и пиримидиновых оснований Т и C [19].…”

Recent Trends on Electrochemical Sensors Based on Ordered Mesoporous Carbon

“…OMC materials by themselves are attractive electrode substrates for electrochemical sensing owing to their attractive properties (large conducting surface area, regular and widely open nanostructure, catalytic edge-plane-like sites, or oxygenated surface functions). Table 1 gathers all applications of unmodified OMC-based electrochemical sensors reported to date [ 23 , 25 , 43 , 44 , 45 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , <...>…”