Direct Dna Amplification from Fall Armyworm (Lepidoptera: Noctuidae) Samples

Abstract

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A new cost‐effective and fast direct PCR protocol for insects based on PBS buffer

“…The taxonomic orders which produced high success rates agreed with previous studies (Armstrong & Ball, ; Loto et al, ). This study was the first time Phasmatodea and thick exoskeleton taxa (e.g.…”

“…The taxonomic orders which produced high success rates agreed with previous studies (Armstrong & Ball, 2005;Loto et al, 2013).…”

“…fruit flies, nonbiting midges and mosquitoes) and Lepidoptera (e.g. fall armyworm) (Grevelding et al, 1996;Loto, Romero, Baigori, & Pera, 2013;Werblow et al, 2016;Wong et al, 2014). Direct PCR also shows promising results in amplifying DNA from ethanol-preserved samples (Loto et al, 2013;Shokralla, Singer, & Hajibabaei, 2010).…”

“…fall armyworm) (Grevelding et al, 1996;Loto, Romero, Baigori, & Pera, 2013;Werblow et al, 2016;Wong et al, 2014). Direct PCR also shows promising results in amplifying DNA from ethanol-preserved samples (Loto et al, 2013;Shokralla, Singer, & Hajibabaei, 2010). However, the numbers of samples in these studies were limited and many taxonomic groups still failed to amplify, especially hard-bodied insects such as Coleoptera and Odonata (Wong et al, 2014).…”

Spodoptera frugiperda (Lepidoptera: Noctuidae) Strains from Northern Argentina: Esterases, Profiles, and Susceptibility to Bacillus thuringiensis (Bacillales: Bacillaceae)

“…Accordingly, in this work, both S. frugiperda biotypes were properly identified by the presence of diagnostic mitochondrial markers before the susceptibility assays were conducted. It is interesting to note that although these results were obtained using isolated DNA, the strain characterization also may be done by amplifying DNA directly from S. frugiperda tissues, such as eggs or neonate larva that is either frozen or ethanol preserved (Loto et al 2013). The use of ethanol-preserved larvae is very attractive, especially in those cases where the sample is required to maintain optimum conditions for extended periods, e.g., during the collection of insect specimens.…”