Direct discrimination of cell surface glycosylation signatures using a single pH-responsive boronic acid-functionalized polymer

Jiang, Mingdi; Chattopadhyay, Aritra Nath; Li, Cheng Hsuan; Geng, Yingying; Luther, David C.; Huang, Rui; Rotello, Vincent

doi:10.1039/d2sc02116a

Cited by 9 publications

(15 citation statements)

References 58 publications

(70 reference statements)

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“…37,38 Recently, we developed a fully synthetic polymeric sensor array (PONI-BA-Pyrene) to successfully discriminate different cell states based on cell surface glycosylation signatures. 39 The single fully synthetic polymer sensor is stable and suitable for highthroughput-high content screening (HT-HCS). We report here the use of this single polymer-based sensor array to detect drug-induced macrophage repolarization from M1 to different M2 subtypes in minutes (Scheme 1).…”

Section: T H Imentioning

confidence: 99%

“…The poly-(oxanorborneneimide)-boronic acid-pyrene (PONI-BA-pyrene) polymer was synthesized and characterized according to a previous report. 39 ■ RESULTS AND DISCUSSION Sensor Array Fabrication. The PONI-BA-pyrene polymer was decorated with boronic acid as the recognition element.…”

Section: Polarization and Repolarization Of Macrophagesmentioning

confidence: 99%

“…Cell surface glycosylation, as an important modulator of cell signaling and regulation, can provide “fingerprints” for assessing cell phenotypes and states. , Recently, we developed a fully synthetic polymeric sensor array ( PONI-BA-Pyrene ) to successfully discriminate different cell states based on cell surface glycosylation signatures . The single fully synthetic polymer sensor is stable and suitable for high-throughput-high content screening (HT-HCS).…”

Section: Introductionmentioning

confidence: 99%

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Sensor Array-Enabled Identification of Drugs for Repolarization of Macrophages to Anti-Inflammatory Phenotypes

et al. 2023

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Macrophages are key components of the innate immune system that have essential functions in physiological processes and diseases. The phenotypic plasticity of macrophages allows cells to be polarized into a multidimensional spectrum of phenotypes, broadly classed as pro-inflammatory (M1) and anti-inflammatory (M2) states. Repolarization of M1 to M2 phenotypes alters the immune response to ameliorate autoimmune and inflammation-associated diseases. Detection of this repolarization, however, is challenging to execute in high-throughput applications. In this work, we demonstrate the ability of a single polymer fabricated to provide a six-channel sensor array that can determine macrophage polarization phenotypes. This sensing platform provides a sensitive and high-throughput tool for detecting drug-induced M1-to-M2 repolarization, allowing the identification of new therapeutic leads for inflammatory diseases. The ability of this sensor array to discriminate different M2 subtypes induced by drugs can also improve the efficacy evaluation of anti-inflammatory drugs and avoid adverse effects.

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Section: T H Imentioning

confidence: 99%

Section: Polarization and Repolarization Of Macrophagesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sensor Array-Enabled Identification of Drugs for Repolarization of Macrophages to Anti-Inflammatory Phenotypes

et al. 2023

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“…[9][10][11] Sensor arrays have emerged as an efficient tool for detecting bioanalytes, including proteins, [12,13] bacteria [14,15] and cells. [16,17] Sensor arrays can be fabricated using a variety of materials, [18] such as gold nanoparticles (AuNPs), [19] polymers [20] and small molecules. [21] AuNPs present a particularly versatile platform for sensor arrays that can be readily modified with chemical or biological molecules for different applications.…”

Section: Introductionmentioning

confidence: 99%

Identification of Proteins Using Supramolecular Gold Nanoparticle‐Dye Sensor Arrays

et al. 2023

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The rapid detection of proteins is very important in the early diagnosis of diseases. Gold nanoparticles (AuNPs) can be engineered to bind biomolecules efficiently and differentially. Cross‐reactive sensor arrays have high sensitivity for sensing proteins using differential interactions between sensor elements and bioanalytes. A new sensor array was fabricated using surface‐charged AuNPs with dyes supramolecularly encapsulated into the AuNP monolayer. The fluorescence of dyes is partially quenched by the AuNPs and can be restored or further quenched due to the differential interactions between AuNPs with proteins. This sensing system enables the discrimination of proteins in both buffer and human serum, providing a potential tool for real‐world disease diagnostics.

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“…Boric acid is a promising candidate as a pH-sensitive agent. [18][19][20] To date, a lot of boronic acid-based hydrogels have been designed and applied in the biological field for pH-regulated healing. [21][22][23][24] Additionally, the pH-controlled interaction between boronic acid and diol moieties is of great convenience for saccharide and nucleic acid sensing.…”

Section: Introductionmentioning

confidence: 99%

A latent reversible ratiometric optical pH sensing probe based on phenylboronic acid for alkaline pH detection and applications in test paper and alkalotic HK-2 cells

Fang,

Xu,

Gong

et al. 2023

New J. Chem.

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Direct discrimination of cell surface glycosylation signatures using a single pH-responsive boronic acid-functionalized polymer

Abstract: Cell surface glycans serve fundamental roles in many biological processes, including cell-cell interaction, pathogen infection, and cancer metastasis. Cancer cell surface have alternative glycosylation to healthy cells, making these changes...

Cited by 9 publications

References 58 publications

Sensor Array-Enabled Identification of Drugs for Repolarization of Macrophages to Anti-Inflammatory Phenotypes

Sensor Array-Enabled Identification of Drugs for Repolarization of Macrophages to Anti-Inflammatory Phenotypes

Identification of Proteins Using Supramolecular Gold Nanoparticle‐Dye Sensor Arrays

A latent reversible ratiometric optical pH sensing probe based on phenylboronic acid for alkaline pH detection and applications in test paper and alkalotic HK-2 cells

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