Direct digitization system for quantification in ion microscopy

Furman, B. K.; Morrison, George H.

doi:10.1021/ac50064a016

Cited by 60 publications

(29 citation statements)

References 19 publications

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“…One of the major interests of AIM studies using the IMS 3F is the capacity of the system to perform element quantitative measurements which appear to be the complement of imaging. Thus, AIM, in association with image processing [8,18] which is in progress in our laboratory [22], opens up new possibilities for the study of the dynamics of physiological and pathophysiological events.…”

Section: Discussionmentioning

confidence: 99%

Changes in iodine mapping in rat thyroid during the course of iodine deficiency: imaging and relative quantitation by analytical ion microscope

Fragu¹

1988

Biology of the Cell

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The analytical ion microscope (AIM) makes possible imaging and relative quantitation of multiple stable or labeled elements on an even tissue section, according to their mass. The purpose of this work was to follow at the rat thyroid follicle level the changes in 127I mapping during low iodine diet (LID) in relation to the ability of thyroid to pick up radioiodine (129I) and to synthesize Tg from its precursor, 2H-labeled leucine. The overall picture of images and countings of 127I shows a progressive decrease of the luminal iodine concentration which on day 80 was 10-fold lower than that of control value. In control rat thyroid cell, concentration was 10-fold lower than that of follicular lumina and was unchanged until 35 days, but the size of the cytoplasmic compartment increased, suggesting a redistribution of iodine stores between thyroid cells and follicular lumina. 129I was always found in colloid as well as in cells at all stages. After 35 days of LID, cytoplasmic and luminal radioiodine concentrations decreased. In control rats, [2H]leucine was found mainly in the cells. During LID its localization was evidenced progressively in most of the lumina. The most striking fact was the presence up to 35 days of some large residual follicles with high 127I concentration and low 129I and 2H incorporation. These data demonstrate the follicular heterogeneity of thyroid response to progressive chronic TSH stimulation induced by LID.

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Section: Discussionmentioning

confidence: 99%

Changes in iodine mapping in rat thyroid during the course of iodine deficiency: imaging and relative quantitation by analytical ion microscope

Fragu¹

1988

Biology of the Cell

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“…The digital ion imaging system includes several novel features or improvements relative to the only previously published approach to digital imaging with the ion microscope (Furman & Morrison, 1980) including: (1) ultrahigh detection sensitivity is achieved via the use of a dual microchannel plate detector and a digital CID camera; (2) a novel interactively compiled BASIC with assembly language extensions is used to combine the execution efficiency of complied language with the interactive features of interpreted language; (3) correlative digital microscopy is achieved by overlaying morphological information (e.g. digitized light micrographs) and chemical information (digital secondary ion images).…”

Section: Discussionmentioning

confidence: 99%

“…Ion microscopes and microprobes are capable of generating five-dimensional information about the near surface composition of solids: three spatial dimensions, ion intensity (elemental concentration), and ion identity (elemental composition). In order to fully access this multi-dimensional data, secondary ion images must be digitized and stored in computer memory (Furman & Morrison, 1980;Rudenauer & Steiger, 1981). This also facilitates post-acquisition image processing, including image enhancement, image reconstruction, image quantification, and multi-image correlation.…”

Section: Introductionmentioning

confidence: 99%

A microcomputer based digital imaging system for ion microanalysis

Bryan

Woodward

Griffis

et al. 1985

Journal of Microscopy

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SUMMARY A microcomputer based digital imaging system was developed for a Cameca IMS‐3f ion microscope permitting real‐time digital acquisition of secondary ion images. Image signal‐to‐noise enhancement results from random noise reduction by real‐time ensemble averaging and from a reduction of pattern noise in the charge injection device (CID) array by subtraction of blank frames. Acquired images comprise 244times248 pixel arrays with 8‐bit intensity resolution. Images are displayed on a colour monitor in a grey scale or pseudo‐colour using one of four programmable lookup tables. Image processing software permits off‐line ion image enhancement and manipulation as well as multitechnique digital image correlations. System capability is illustrated by a biological example involving digital imaging studies of Al distribution in osteomalacic bone tissue, including correlative light microscopy and ion microanalysis.

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“…intensified silicon-intensified target video camera (MIDAS system) (Furman & Morrison, 1980) and recorded on videotape using the VICAR system as described (Brcnna et aI., 1986) except for the use of a Trapix 5500 series (Recognition Concepts, Inc.) frame buffer. Images were digitized from the VICAR system off-line using prcviously described vASGPD software which had been modified (Brcnna er a/., 1986).…”

Section: Ion Microscopy and Image Analysismentioning

confidence: 99%

Biological cryosection preparation and practical ion yield evaluation for ion microscopic analysis

Sod

Crooker

Morrison

1990

Journal of Microscopy

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SUMMARY A mounting method utilizing an indium substrate is described for preparing freeze‐dried cryosections of biological tissue for ion microscopic analysis. Using this procedure, a qualitative comparison between cryosection, conventional chemical, and freeze‐substitution specimen preparations is made with rat liver tissue. Practical ion yield variations in cryosections are found to be minimal (± 13% relative standard deviation) in tissue‐containing areas of rat liver and small intestine.

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Direct digitization system for quantification in ion microscopy

Cited by 60 publications

References 19 publications

Changes in iodine mapping in rat thyroid during the course of iodine deficiency: imaging and relative quantitation by analytical ion microscope

Changes in iodine mapping in rat thyroid during the course of iodine deficiency: imaging and relative quantitation by analytical ion microscope

A microcomputer based digital imaging system for ion microanalysis

Biological cryosection preparation and practical ion yield evaluation for ion microscopic analysis

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