Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles

Study on the ternary system of MoO42‐–enzyme–PdCl2 by resonance Rayleigh scattering, second‐order scattering and frequency‐doubling scattering spectra and its analytical application

“…Under optimum conditions, the relative scattering intensities ΔI RRS , ΔI SOS and ΔI FDS of the ternary complex were measured at their maximum scattering wavelengths. RRS was the most sensitive of the methods, the detection limit was lower than that of the spectrophotometric method (22), spectrofluorimetric method (23), electrogenerated chemiluminescence method (24), chemiluminescence method (25), electrochemical method (26), plasmon resonance light-scattering method (27) and RRS method with Au nanoparticle as a probe (17) ( Table 3). The regression equation, linear range, correlation coefficient and detection limit are listed in Table 2.…”

“…The detection limits (3s) of LYSO and PAP were 4.5 and 14.0 ng/mL (RRS method), 9.6 and 57.8 ng/mL (SOS method), and 5.2 and 106.0 ng/mL (FDS method). RRS was the most sensitive of the methods, the detection limit was lower than that of the spectrophotometric method (22), spectrofluorimetric method (23), electrogenerated chemiluminescence method (24), chemiluminescence method (25), electrochemical method (26), plasmon resonance light-scattering method (27) and RRS method with Au nanoparticle as a probe (17) ( Table 3). Therefore, the method was very suitable for determining trace amounts of LYSO (or PAP).…”

Detection of urinary creatinine using gold nanoparticles after solid phase extraction

“…The interaction between gold nanoparticles and biomolecules may result in the aggregation or disaggregation of particles, depending on the experimental design and conditions. Gold nanoparticles can interact with molecules or ions via electrostatic interactions or interactions with sulfur-containing moieties of the target molecules via covalent bonding [16][17][18][19]. These interactionsare non-specific and therefore, in the analysis of complex matrix samples (e.g.…”

Heat induced aggregation of gold nanorods for rapid visual detection of lysozyme

“…Therefore, development of a simple technique for monitoring this protein could be very important in diagnostic approaches. Recently, Xinyi Wang et al established a simple and sensitive analytical method for lysozyme, using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe [20]. They highlighted a number of traditional methods such as turbidimetric detection, lysoplate assay, lysorocket electrophoresis and enzyme-linked immunosorbent assay (ELISA) for estimation of lysozyme; where each technique has its own problem of sensitivity, specificity, complex modification process, essence of sophisticated separations, expensive instrumentation, poor reproducibility and time consuming analysis [20].…”